Restriction map for
pF1KA0772
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 1189 CGCG CG^CG
AflIII 302 ACGTGT A^C[GA][CT]GT
AlwNI 337 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 447 ATTAAT AT^TAAT
AsnI 447 ATTAAT AT^TAAT
AspHI 298 GAGCAC G[AT]GC[AT]^C
AvaI 608 CCCGAG C^[CT]CG[GA]G
BalI 1217 TGGCCA TGG^CCA
BanI 613 GGCACC G^G[CT][GA]CC
BanII 604 GAGCCC G[GA]GC[CT]^C
BbrPI 301 CACGTG CAC^GTG
BsaAI 301 CACGTG [CT]AC^GT[GA]
BsgI 1024 GTGCAG GTGCAG(16/14)
BsiHKAI 298 GAGCAC G[AT]GC[AT]^C
BsoBI 608 CCCGAG C^[CT]CG[GA]G
BstEII 934 GGTGACC G^GT[ACGT]ACC
BstPI 934 GGTGACC G^GT[ACGT]ACC
BstUI 1189 CGCG CG^CG
CfoI 1176 GCGC GCG^C
DraIII 805 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1217 TGGCCA [CT]^GGCC[GA]
EcoNI 942 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 934 GGTGACC G^GT[ACGT]ACC
EcoRV 1396 GATATC GAT^ATC
FauI 1272 GCGGG CCCGC(4/6)
HgaI 677 GCGTC GACGC(5/10)
HhaI 1176 GCGC GCG^C
HinP1I 1176 GCGC G^CGC
KspI 1188 CCGCGG CCGC^GG
MluNI 1217 TGGCCA TGG^CCA
MscI 1217 TGGCCA TGG^CCA
MvnI 1189 CGCG CG^CG
NaeI 156 GCCGGC GCC^GGC
NcoI 552 CCATGG C^CATGG
NgoMIV 156 GCCGGC G^CCGGC
PflMI 336 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 967 GACTC GAGTC(4/5)
PmaCI 301 CACGTG CAC^GTG
PmlI 301 CACGTG CAC^GTG
PpuMI 1336 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 447 ATTAAT AT^TAAT
SacII 1188 CCGCGG CCGC^GG
Van91I 336 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 291 GTAC GT^AC
306 GTAC
BbsI 964 GAAGAC GAAGAC(2/6)
1146 GAAGAC
BcnI 971 CCGGG CC^[GC]GG
1067 CCCGG
BmyI 298 GAGCAC G[AGT]GC[ACT]^C
604 GAGCCC
BpuAI 964 GAAGAC GAAGAC(2/6)
1146 GAAGAC
BsaBI 240 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
473 GATTTATATC
BsaI 1018 GAGACC GGTCTC(1/5)
529 GGTCTC
BsmFI 721 GGGAC GGGAC(10/14)
1336 GGGAC
Bsp1286I 298 GAGCAC G[AGT]GC[ACT]^C
604 GAGCCC
BspMI 670 ACCTGC ACCTGC(4/8)
1026 GCAGGT
BsrFI 156 GCCGGC [GA]^CCGG[CT]
406 ACCGGC
BstXI 617 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
741 CCACAGAACTGG
BstYI 891 AGATCC [GA]^GATC[CT]
1212 GGATCT
Cfr10I 156 GCCGGC [GA]^CCGG[CT]
406 ACCGGC
DraI 735 TTTAAA TTT^AAA
772 TTTAAA
EcoRI 16 GAATTC G^AATTC
131 GAATTC
EcoT14I 552 CCATGG C^C[AT][AT]GG
1277 CCAAGG
MamI 240 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
473 GATTTATATC
MflI 891 AGATCC [GA]^GATC[CT]
1212 GGATCT
MslI 54 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
754 CATAAGTGTG
MspA1I 277 CAGCGG C[AC]G^C[GT]G
1188 CCGCGG
NciI 971 CCGGG CC^[GC]GG
1067 CCCGG
RsaI 291 GTAC GT^AC
306 GTAC
SmlI 119 CTCAAG C^T[CT][GA]AG
574 CTCAAG
StyI 552 CCATGG C^C[AT][AT]GG
1277 CCAAGG
TfiI 467 GATTC G^A[AT]TC
907 GAATC
XhoII 891 AGATCC [GA]^GATC[CT]
1212 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsrBI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI Tsp45I Tsp509I TspRI