Restriction map for
pF1KA0810
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI AspEI
AvrII BbeI BbvCI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BseRI BsiWI BsmI
BspDI BspEI BspHI BsrDI BssHII
BssSI BstEII BstPI Bsu36I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI HincII HindII
HindIII HpaI KasI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SfiI SfoI SgrAI
SnaBI SpeI SspI StuI SwaI
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 2391 GTATAC GT^[AC][GT]AC
Aor51HI 235 AGCGCT AGC^GCT
ApaI 616 GGGCCC GGGCC^C
Asp700I 1396 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2175 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 761 TGCGCA TGC^GCA
BalI 173 TGGCCA TGG^CCA
BamHI 2015 GGATCC G^GATCC
BanII 616 GGGCCC G[GA]GC[CT]^C
BbsI 1566 GAAGAC GAAGAC(2/6)
BclI 2283 TGATCA T^GATCA
BpuAI 1566 GAAGAC GAAGAC(2/6)
BsaBI 463 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 734 GTGCAG GTGCAG(16/14)
BsiEI 699 CGGTCG CG[GA][CT]^CG
Bsp1407I 23 TGTACA T^GTACA
BspLU11I 20 ACATGT A^CATGT
BsrBI 257 GAGCGG CCGCTC(-3/-3)
BsrFI 1570 ACCGGC [GA]^CCGG[CT]
BsrGI 23 TGTACA T^GTACA
Bst1107I 2391 GTATAC GTA^TAC
BstBI 938 TTCGAA TT^CGAA
BstXI 1209 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2391 GTATAC GTA^TAC
Cfr10I 1570 ACCGGC [GA]^CCGG[CT]
DraI 2092 TTTAAA TTT^AAA
EarI 976 CTCTTC CTCTTC(1/4)
Eco47III 235 AGCGCT AGC^GCT
EcoT22I 1057 ATGCAT ATGCA^T
FbaI 2283 TGATCA T^GATCA
FspI 761 TGCGCA TGC^GCA
Ksp632I 976 CTCTTC CTCTTC(1/4)
KspI 605 CCGCGG CCGC^GG
MamI 463 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 173 TGGCCA TGG^CCA
MscI 173 TGGCCA TGG^CCA
NcoI 1215 CCATGG C^CATGG
NsiI 1057 ATGCAT ATGCA^T
NspV 938 TTCGAA TT^CGAA
PflFI 2175 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2346 CCAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 709 GGGACCC [GA]G^G[AT]CC[CT]
SacII 605 CCGCGG CCGC^GG
SbfI 369 CCTGCAGG CCTGCA^GG
ScaI 1960 AGTACT AGT^ACT
SfuI 938 TTCGAA TT^CGAA
SmaI 613 CCCGGG CCC^GGG
SmlI 1956 CTTGAG C^T[CT][GA]AG
Sse8387I 369 CCTGCAGG CCTGCA^GG
SspBI 23 TGTACA T^GTACA
Tth111I 2175 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2346 CCAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 613 CCCGGG C^CCGGG
XmaI 613 CCCGGG C^CCGGG
XmnI 1396 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 881 GGTACC G^GTACC
2108 GGTACC
AcyI 343 GGCGTC G[GA]^CG[CT]C
1839 GGCGTC
Asp718I 881 GGTACC G^GTACC
2108 GGTACC
BcgI 940 CGAAACATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
693 GCACCCCGGTCG
BsaHI 343 GGCGTC G[GA]^CG[CT]C
1839 GGCGTC
BsaI 1736 GAGACC GGTCTC(1/5)
994 GGTCTC
BsmBI 109 GAGACG CGTCTC(1/5)
1316 GAGACG
BstYI 1751 AGATCC [GA]^GATC[CT]
2015 GGATCC
DraII 709 GGGACCC [GA]G^G[ACGT]CC[CT]
2315 AGGCCCT
EcoO109I 709 GGGACCC [GA]G^G[ACGT]CC[CT]
2315 AGGCCCT
EcoT14I 1215 CCATGG C^C[AT][AT]GG
2210 CCAAGG
Hin1I 343 GGCGTC G[GA]^CG[CT]C
1839 GGCGTC
KpnI 881 GGTACC GGTAC^C
2108 GGTACC
MflI 1751 AGATCC [GA]^GATC[CT]
2015 GGATCC
MseI 473 TTAA T^TAA
2093 TTAA
MslI 1210 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1213 CACCATGGTG
SexAI 928 ACCAGGT A^CC[AT]GGT
2111 ACCTGGT
StyI 1215 CCATGG C^C[AT][AT]GG
2210 CCAAGG
Tru9I 473 TTAA T^TAA
2093 TTAA
Tsp509I 122 AATT ^AATT
1018 AATT
XhoII 1751 AGATCC [GA]^GATC[CT]
2015 GGATCC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BbrPI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DrdI DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI TaqI TfiI
TseI Tsp45I TspRI TthHB8I