Restriction map for
pF1KA0838
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstZ17I CelII CpoI
DrdI Eco47III EcoNI EcoRV FspI
HindIII HpaI KpnI MamI MroI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XcmI XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AclI 1128 AACGTT AA^CGTT
AflIII 338 ACGCGT A^C[GA][CT]GT
AhdI 667 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 374 CCTCAGG CC^T[ACGT]AGG
ApaI 138 GGGCCC GGGCC^C
Asp700I 1351 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 667 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1066 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 33 GGACC G^G[AT]CC
BalI 758 TGGCCA TGG^CCA
BanI 303 GGCGCC G^G[CT][GA]CC
BbeI 303 GGCGCC GGCGC^C
BclI 1641 TGATCA T^GATCA
BglI 295 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1502 GGTCTC GGTCTC(1/5)
BsmBI 331 GAGACG CGTCTC(1/5)
Bsp1407I 796 TGTACA T^GTACA
BspDI 917 ATCGAT AT^CGAT
BspMI 35 ACCTGC ACCTGC(4/8)
BsrDI 882 CATTGC GCAATG(2/0)
BsrGI 796 TGTACA T^GTACA
BstEII 93 GGTCACC G^GT[ACGT]ACC
BstNI 518 CCAGG CC^[AT]GG
BstPI 93 GGTCACC G^GT[ACGT]ACC
Bsu36I 374 CCTCAGG CC^T[ACGT]AGG
ClaI 917 ATCGAT AT^CGAT
DraIII 96 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 667 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 374 CCTCAGG CC^T[ACGT]AGG
EcoO65I 93 GGTCACC G^GT[ACGT]ACC
EcoRI 1541 GAATTC G^AATTC
EcoRII 518 CCAGG ^CC[AT]GG
EcoT22I 1378 ATGCAT ATGCA^T
FbaI 1641 TGATCA T^GATCA
FseI 128 GGCCGGCC GGCCGG^CC
HaeII 303 GGCGCC [GA]GCGC^[CT]
HincII 630 GTTGAC GT[CT]^[GA]AC
HindII 630 GTTGAC GT[CT]^[GA]AC
KasI 303 GGCGCC G^GCGCC
KspI 134 CCGCGG CCGC^GG
MluI 338 ACGCGT A^CGCGT
MluNI 758 TGGCCA TGG^CCA
MscI 758 TGGCCA TGG^CCA
MvaI 518 CCAGG CC^[AT]GG
NarI 303 GGCGCC GG^CGCC
NcoI 1868 CCATGG C^CATGG
NsiI 1378 ATGCAT ATGCA^T
NspI 1391 GCATGT [GA]CATG^[CT]
PflFI 1066 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1321 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 32 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1128 AACGTT AA^CGTT
PstI 1781 CTGCAG CTGCA^G
PvuII 1246 CAGCTG CAG^CTG
SacII 134 CCGCGG CCGC^GG
ScaI 1338 AGTACT AGT^ACT
SfoI 303 GGCGCC GGC^GCC
SgrAI 54 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1943 CTCAAG C^T[CT][GA]AG
SspBI 796 TGTACA T^GTACA
Tth111I 1066 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1321 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1756 TCTAGA T^CTAGA
XmnI 1351 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcyI 107 GGCGTC G[GA]^CG[CT]C
303 GGCGCC
AlwI 514 GATCC GGATC(4/5)
1621 GATCC
AlwNI 241 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
397 CAGGGTCTG
AspHI 258 GAGCAC G[AT]GC[AT]^C
1251 GTGCTC
BbsI 848 GTCTTC GAAGAC(2/6)
1430 GTCTTC
BglII 233 AGATCT A^GATCT
591 AGATCT
BpuAI 848 GTCTTC GAAGAC(2/6)
1430 GTCTTC
BsaHI 107 GGCGTC G[GA]^CG[CT]C
303 GGCGCC
BseRI 1505 CTCCTC GAGGAG(10/8)
1508 CTCCTC
BsgI 1700 CTGCAC GTGCAG(16/14)
1882 CTGCAC
BsiHKAI 258 GAGCAC G[AT]GC[AT]^C
1251 GTGCTC
BstBI 1358 TTCGAA TT^CGAA
1706 TTCGAA
BstXI 1280 CCAGTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1478 CCAATGTTATGG
BstYI 233 AGATCT [GA]^GATC[CT]
591 AGATCT
DraI 598 TTTAAA TTT^AAA
1906 TTTAAA
DraII 32 GGGACCT [GA]G^G[ACGT]CC[CT]
209 GGGGCCT
EagI 127 CGGCCG C^GGCCG
131 CGGCCG
EarI 554 CTCTTC CTCTTC(1/4)
1628 GAAGAG
EclXI 127 CGGCCG C^GGCCG
131 CGGCCG
Eco52I 127 CGGCCG C^GGCCG
131 CGGCCG
EcoO109I 32 GGGACCT [GA]G^G[ACGT]CC[CT]
209 GGGGCCT
HgaI 337 GACGC GACGC(5/10)
108 GCGTC
Hin1I 107 GGCGTC G[GA]^CG[CT]C
303 GGCGCC
Ksp632I 554 CTCTTC CTCTTC(1/4)
1628 GAAGAG
MaeII 508 ACGT A^CGT
1129 ACGT
MfeI 437 CAATTG C^AATTG
1013 CAATTG
MflI 233 AGATCT [GA]^GATC[CT]
591 AGATCT
MunI 437 CAATTG C^AATTG
1013 CAATTG
NspV 1358 TTCGAA TT^CGAA
1706 TTCGAA
PleI 69 GACTC GAGTC(4/5)
158 GACTC
SfcI 494 CTACAG C^T[GA][CT]AG
1781 CTGCAG
SfuI 1358 TTCGAA TT^CGAA
1706 TTCGAA
SmaI 274 CCCGGG CCC^GGG
325 CCCGGG
XhoII 233 AGATCT [GA]^GATC[CT]
591 AGATCT
XmaCI 274 CCCGGG C^CCGGG
325 CCCGGG
XmaI 274 CCCGGG C^CCGGG
325 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I