Restriction map for
pF1KA0917
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpuAI BsaAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BstEII BstPI BstUI BstXI
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 265 GTATAC GT^[AC][GT]AC
Alw44I 841 GTGCAC G^TGCAC
AlwNI 1328 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 841 GTGCAC G^TGCAC
Asp700I 1015 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1508 CCTCAGC CCTCAGC(-5/-2)
BlpI 1138 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1138 GCTAAGC GC^T[ACGT]AGC
BsaBI 235 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 431 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 960 TCCGGT [AT]^CCGG[AT]
BsgI 413 GTGCAG GTGCAG(16/14)
BsmAI 197 GAGAC GTCTC(1/5)
BsmFI 1086 GGGAC GGGAC(10/14)
BspHI 1568 TCATGA T^CATGA
BsrDI 1727 GCAATG GCAATG(2/0)
BssSI 1562 CACGAG CACGAG(-5/-1)
Bst1107I 265 GTATAC GTA^TAC
BstAPI 406 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 317 TTCGAA TT^CGAA
BstZ17I 265 GTATAC GTA^TAC
CelII 1138 GCTAAGC GC^T[ACGT]AGC
Cfr13I 764 GGCCC G^G[ACGT]CC
DraII 763 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1333 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 763 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 538 GATATC GAT^ATC
EcoT14I 431 CCAAGG C^C[AT][AT]GG
FauI 1722 GCGGG CCCGC(4/6)
HindIII 1433 AAGCTT A^AGCTT
HphI 727 GGTGA GGTGA(8/7)
MamI 235 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NlaIV 1108 GGAGCC GG[ACGT]^[ACGT]CC
NspV 317 TTCGAA TT^CGAA
PleI 1565 GAGTC GAGTC(4/5)
RcaI 1568 TCATGA T^CATGA
Sau96I 764 GGCCC G^G[ACGT]CC
SexAI 1610 ACCTGGT A^CC[AT]GGT
SfuI 317 TTCGAA TT^CGAA
SmlI 1177 CTTGAG C^T[CT][GA]AG
SspI 292 AATATT AAT^ATT
StyI 431 CCAAGG C^C[AT][AT]GG
SwaI 344 ATTTAAAT ATTT^AAAT
Tsp45I 1908 GTCAC ^GT[GC]AC
XbaI 1314 TCTAGA T^CTAGA
XmnI 1015 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 133 GTAC GT^AC
850 GTAC
AspHI 841 GTGCAC G[AT]GC[AT]^C
1046 GAGCAC
BalI 153 TGGCCA TGG^CCA
744 TGGCCA
BmyI 841 GTGCAC G[AGT]GC[ACT]^C
1046 GAGCAC
BpmI 908 CTCCAG CTGGAG(16/14)
1346 CTCCAG
BseRI 170 CTCCTC GAGGAG(10/8)
1790 GAGGAG
BsiHKAI 841 GTGCAC G[AT]GC[AT]^C
1046 GAGCAC
Bsp1286I 841 GTGCAC G[AGT]GC[ACT]^C
1046 GAGCAC
BssKI 309 CCAGG ^CC[ACGT]GG
1611 CCTGG
BstF5I 1254 GGATG GGATG(2/0)
1450 GGATG
BstNI 309 CCAGG CC^[AT]GG
1611 CCTGG
BstYI 240 AGATCC [GA]^GATC[CT]
312 GGATCT
DraI 345 TTTAAA TTT^AAA
864 TTTAAA
EarI 589 CTCTTC CTCTTC(1/4)
1310 CTCTTC
EcoRII 309 CCAGG ^CC[AT]GG
1611 CCTGG
FokI 1254 GGATG GGATG(9/13)
1450 GGATG
HapII 961 CCGG C^CGG
1516 CCGG
HpaII 961 CCGG C^CGG
1516 CCGG
Ksp632I 589 CTCTTC CTCTTC(1/4)
1310 CTCTTC
MflI 240 AGATCC [GA]^GATC[CT]
312 GGATCT
MluNI 153 TGGCCA TGG^CCA
744 TGGCCA
MscI 153 TGGCCA TGG^CCA
744 TGGCCA
MspA1I 911 CAGCTG C[AC]G^C[GT]G
1151 CAGCTG
MspI 961 CCGG C^CGG
1516 CCGG
MvaI 309 CCAGG CC^[AT]GG
1611 CCTGG
NlaIII 201 CATG CATG^
1569 CATG
PstI 260 CTGCAG CTGCA^G
1866 CTGCAG
PvuII 911 CAGCTG CAG^CTG
1151 CAGCTG
RsaI 133 GTAC GT^AC
850 GTAC
ScrFI 309 CCAGG CC^[ACGT]GG
1611 CCTGG
XcmI 1538 CCACTAAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1772 CCATTGTTTTTGTGG
XhoII 240 AGATCC [GA]^GATC[CT]
312 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BsiYI BslI BsrI
Cac8I DdeI DpnII EaeI Fnu4HI
HaeIII HincII HindII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII Sau3AI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I