Restriction map for
pF1KA0931
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AvrII BbeI BcgI BciVI BfrI
BlnI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HgaI Hin1I HpaI
KasI KspI MamI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 532 GGTACC G^GTACC
AccII 2282 CGCG CG^CG
Alw44I 2838 GTGCAC G^TGCAC
AocI 1150 CCTGAGG CC^T[ACGT]AGG
ApaLI 2838 GTGCAC G^TGCAC
Asp718I 532 GGTACC G^GTACC
AspI 274 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 359 TGCGCA TGC^GCA
BanI 532 GGTACC G^G[CT][GA]CC
BbvCI 1413 GCTGAGG CCTCAGC(-5/-2)
BclI 2100 TGATCA T^GATCA
BglI 3138 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 959 AGATCT A^GATCT
Bsp1407I 2662 TGTACA T^GTACA
BsrDI 8 GCAATG GCAATG(2/0)
BsrFI 3701 GCCGGC [GA]^CCGG[CT]
BsrGI 2662 TGTACA T^GTACA
BstAPI 582 GCACATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2634 GGTAACC G^GT[ACGT]ACC
BstPI 2634 GGTAACC G^GT[ACGT]ACC
BstUI 2282 CGCG CG^CG
BstXI 3614 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1150 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3701 GCCGGC [GA]^CCGG[CT]
CpoI 3733 CGGACCG CG^G[AT]CCG
DraIII 1427 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1150 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2634 GGTAACC G^GT[ACGT]ACC
EcoRI 1628 GAATTC G^AATTC
FbaI 2100 TGATCA T^GATCA
FspI 359 TGCGCA TGC^GCA
HindIII 1834 AAGCTT A^AGCTT
KpnI 532 GGTACC GGTAC^C
MfeI 1804 CAATTG C^AATTG
MunI 1804 CAATTG C^AATTG
MvnI 2282 CGCG CG^CG
NaeI 3701 GCCGGC GCC^GGC
NgoMIV 3701 GCCGGC G^CCGGC
PflFI 274 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1644 GAGTC GAGTC(4/5)
RsrII 3733 CGGACCG CG^G[AT]CCG
SexAI 275 ACCTGGT A^CC[AT]GGT
SphI 3440 GCATGC GCATG^C
SspBI 2662 TGTACA T^GTACA
SspI 2899 AATATT AAT^ATT
Tsp45I 2801 GTCAC ^GT[GC]AC
Tth111I 274 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 921 CCATAATAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 1951 GTAGAC GT^[AC][GT]AC
3304 GTAGAC
AlwNI 2780 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2948 CAGGTCCTG
BalI 2400 TGGCCA TGG^CCA
3569 TGGCCA
BamHI 2681 GGATCC G^GATCC
3410 GGATCC
BbrPI 1327 CACGTG CAC^GTG
2496 CACGTG
BbsI 3416 GAAGAC GAAGAC(2/6)
2548 GTCTTC
BlpI 508 GCTGAGC GC^T[ACGT]AGC
650 GCTCAGC
Bpu1102I 508 GCTGAGC GC^T[ACGT]AGC
650 GCTCAGC
BpuAI 3416 GAAGAC GAAGAC(2/6)
2548 GTCTTC
BsaI 272 GAGACC GGTCTC(1/5)
2261 GAGACC
BsgI 1876 CTGCAC GTGCAG(16/14)
2916 CTGCAC
BspHI 1002 TCATGA T^CATGA
3651 TCATGA
BsrBI 2420 CCGCTC CCGCTC(-3/-3)
3142 GAGCGG
BssSI 1746 CACGAG CACGAG(-5/-1)
768 CTCGTG
CelII 508 GCTGAGC GC^T[ACGT]AGC
650 GCTCAGC
EcoNI 780 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2556 CCTGGAGCAGG
FauI 2280 CCCGC CCCGC(4/6)
1338 GCGGG
HincII 2139 GTCAAC GT[CT]^[GA]AC
2334 GTCAAC
HindII 2139 GTCAAC GT[CT]^[GA]AC
2334 GTCAAC
MluNI 2400 TGGCCA TGG^CCA
3569 TGGCCA
MscI 2400 TGGCCA TGG^CCA
3569 TGGCCA
NcoI 1286 CCATGG C^CATGG
2154 CCATGG
PflMI 2154 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2403 CCAGAAGTTGG
PmaCI 1327 CACGTG CAC^GTG
2496 CACGTG
PmlI 1327 CACGTG CAC^GTG
2496 CACGTG
PpuMI 2564 AGGACCC [GA]G^G[AT]CC[CT]
2949 AGGTCCT
RcaI 1002 TCATGA T^CATGA
3651 TCATGA
SacI 976 GAGCTC GAGCT^C
2275 GAGCTC
StuI 870 AGGCCT AGG^CCT
1775 AGGCCT
Van91I 2154 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2403 CCAGAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoI XhoII