Restriction map for
pF1KA1065
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI BstBI BstYI
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII KasI MamI MfeI
MflI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1147 GGTACC G^GTACC
AccI 1871 GTATAC GT^[AC][GT]AC
Asp718I 1147 GGTACC G^GTACC
AspI 6 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1135 TGGCCA TGG^CCA
BfaI 815 CTAG C^TAG
BlpI 871 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 871 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 221 TGTACA T^GTACA
BspHI 167 TCATGA T^CATGA
BsrDI 1843 GCAATG GCAATG(2/0)
BsrGI 221 TGTACA T^GTACA
Bst1107I 1871 GTATAC GTA^TAC
BstAPI 1621 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1575 GGTGACC G^GT[ACGT]ACC
BstPI 1575 GGTGACC G^GT[ACGT]ACC
BstZ17I 1871 GTATAC GTA^TAC
CelII 871 GCTGAGC GC^T[ACGT]AGC
DraIII 1622 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1135 TGGCCA [CT]^GGCC[GA]
EcoNI 1741 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1575 GGTGACC G^GT[ACGT]ACC
HgaI 1279 GACGC GACGC(5/10)
HincII 1651 GTTAAC GT[CT]^[GA]AC
HindII 1651 GTTAAC GT[CT]^[GA]AC
HpaI 1651 GTTAAC GTT^AAC
KpnI 1147 GGTACC GGTAC^C
MaeI 815 CTAG C^TAG
MluI 1280 ACGCGT A^CGCGT
MluNI 1135 TGGCCA TGG^CCA
MscI 1135 TGGCCA TGG^CCA
NspI 216 GCATGT [GA]CATG^[CT]
PflFI 6 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1324 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 167 TCATGA T^CATGA
SacI 1333 GAGCTC GAGCT^C
SapI 1335 GCTCTTC GCTCTTC(1/4)
ScaI 1120 AGTACT AGT^ACT
SexAI 1623 ACCAGGT A^CC[AT]GGT
SpeI 814 ACTAGT A^CTAGT
SspBI 221 TGTACA T^GTACA
TaqI 1774 TCGA T^CGA
TfiI 1414 GAATC G^A[AT]TC
Tth111I 6 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1774 TCGA T^CGA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcsI 953 AAATTC [GA]^AATT[CT]
1378 GAATTT
AflIII 270 ACGTGT A^C[GA][CT]GT
1280 ACGCGT
AocI 598 CCTGAGG CC^T[ACGT]AGG
888 CCTCAGG
ApoI 953 AAATTC [GA]^AATT[CT]
1378 GAATTT
Asp700I 294 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
318 GAAGGACTTC
AspHI 1333 GAGCTC G[AT]GC[AT]^C
1628 GTGCTC
AvaI 761 CCCGAG C^[CT]CG[GA]G
1029 CTCGGG
BglI 713 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1636 GCCACCTCGGC
BsiHKAI 1333 GAGCTC G[AT]GC[AT]^C
1628 GTGCTC
BsoBI 761 CCCGAG C^[CT]CG[GA]G
1029 CTCGGG
Bsu36I 598 CCTGAGG CC^T[ACGT]AGG
888 CCTCAGG
DpnII 165 GATC ^GATC
495 GATC
EarI 1336 CTCTTC CTCTTC(1/4)
823 GAAGAG
Eco81I 598 CCTGAGG CC^T[ACGT]AGG
888 CCTCAGG
HaeII 228 GGCGCT [GA]GCGC^[CT]
674 AGCGCC
Ksp632I 1336 CTCTTC CTCTTC(1/4)
823 GAAGAG
KspI 1037 CCGCGG CCGC^GG
1784 CCGCGG
MaeII 146 ACGT A^CGT
270 ACGT
MboI 165 GATC ^GATC
495 GATC
NdeII 165 GATC ^GATC
495 GATC
PflMI 1088 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1138 CCATCGTTTGG
PstI 658 CTGCAG CTGCA^G
838 CTGCAG
SacII 1037 CCGCGG CCGC^GG
1784 CCGCGG
Sau3AI 165 GATC ^GATC
495 GATC
SfaNI 1015 GATGC GCATC(5/9)
359 GCATC
SfcI 658 CTGCAG C^T[GA][CT]AG
838 CTGCAG
SmlI 400 CTCAAG C^T[CT][GA]AG
1494 CTCAAG
StuI 602 AGGCCT AGG^CCT
1582 AGGCCT
Van91I 1088 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1138 CCATCGTTTGG
XmnI 294 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
318 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
PleI PvuII RsaI Sau96I ScrFI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI