Restriction map for
pF1KA1091
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccI AccII AclI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BcgI BciVI BlnI BlpI Bpu1102I
BsaAI BsiEI BsiWI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraIII EagI EclXI
Eco52I EcoO65I EcoT22I FseI FspI
HpaI KpnI KspI MluI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3834 GACGTC GACGT^C
AccIII 3266 TCCGGA T^CCGGA
AflII 1387 CTTAAG C^TTAAG
AflIII 3737 ACATGT A^C[GA][CT]GT
AgeI 1109 ACCGGT A^CCGGT
AhdI 97 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3360 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 97 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2725 GAGCTC G[AT]GC[AT]^C
BalI 3039 TGGCCA TGG^CCA
BamHI 3176 GGATCC G^GATCC
BbeI 30 GGCGCC GGCGC^C
BbvCI 697 CCTCAGC CCTCAGC(-5/-2)
BclI 3758 TGATCA T^GATCA
BfrI 1387 CTTAAG C^TTAAG
BglII 434 AGATCT A^GATCT
BsaBI 3461 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3266 TCCGGA T^CCGGA
BseRI 2710 CTCCTC GAGGAG(10/8)
BsgI 3731 CTGCAC GTGCAG(16/14)
BsiHKAI 2725 GAGCTC G[AT]GC[AT]^C
BspEI 3266 TCCGGA T^CCGGA
BspLU11I 3737 ACATGT A^CATGT
BstAPI 3801 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 3463 TTTAAA TTT^AAA
Eam1105I 97 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1256 GAATTC G^AATTC
EcoRV 239 GATATC GAT^ATC
FbaI 3758 TGATCA T^GATCA
HincII 1822 GTTGAC GT[CT]^[GA]AC
HindII 1822 GTTGAC GT[CT]^[GA]AC
HindIII 3019 AAGCTT A^AGCTT
KasI 30 GGCGCC G^GCGCC
MamI 3461 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3039 TGGCCA TGG^CCA
MroI 3266 TCCGGA T^CCGGA
MscI 3039 TGGCCA TGG^CCA
MspA1I 3241 CCGCTG C[AC]G^C[GT]G
NarI 30 GGCGCC GG^CGCC
PinAI 1109 ACCGGT A^CCGGT
PpuMI 227 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 2725 GAGCTC GAGCT^C
SapI 3477 GCTCTTC GCTCTTC(1/4)
SfoI 30 GGCGCC GGC^GCC
SwaI 3462 ATTTAAAT ATTT^AAAT
XmnI 3360 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 186 AAATTT [GA]^AATT[CT]
1256 GAATTC
AcyI 30 GGCGCC G[GA]^CG[CT]C
3834 GACGTC
AlwNI 955 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
3655 CAGATGCTG
Aor51HI 566 AGCGCT AGC^GCT
2747 AGCGCT
ApoI 186 AAATTT [GA]^AATT[CT]
1256 GAATTC
AvaI 877 CTCGGG C^[CT]CG[GA]G
1817 CCCGAG
BglI 300 GCCGAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3706 GCCCTGCTGGC
BsaHI 30 GGCGCC G[GA]^CG[CT]C
3834 GACGTC
BsaWI 1109 ACCGGT [AT]^CCGG[AT]
3266 TCCGGA
BsoBI 877 CTCGGG C^[CT]CG[GA]G
1817 CCCGAG
BspHI 644 TCATGA T^CATGA
1773 TCATGA
BspMI 1703 ACCTGC ACCTGC(4/8)
2123 ACCTGC
BsrBI 754 CCGCTC CCGCTC(-3/-3)
3172 GAGCGG
DraII 227 AGGTCCT [GA]G^G[ACGT]CC[CT]
2255 AGGGCCT
DrdI 49 GACTACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
847 GACTTTCCTGTC
DsaI 2672 CCATGG C^C[GA][CT]GG
2923 CCATGG
Eco47III 566 AGCGCT AGC^GCT
2747 AGCGCT
EcoO109I 227 AGGTCCT [GA]G^G[ACGT]CC[CT]
2255 AGGGCCT
FauI 3237 CCCGC CCCGC(4/6)
1314 GCGGG
HgaI 3399 GACGC GACGC(5/10)
1923 GCGTC
Hin1I 30 GGCGCC G[GA]^CG[CT]C
3834 GACGTC
MfeI 1913 CAATTG C^AATTG
2238 CAATTG
MseI 1388 TTAA T^TAA
3464 TTAA
MunI 1913 CAATTG C^AATTG
2238 CAATTG
NcoI 2672 CCATGG C^CATGG
2923 CCATGG
PleI 2412 GAGTC GAGTC(4/5)
3328 GAGTC
RcaI 644 TCATGA T^CATGA
1773 TCATGA
Tru9I 1388 TTAA T^TAA
3464 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EarI Eco81I EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII