Restriction map for
pF1KA1181
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BseAI BseRI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MslI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 208 GACGTC GACGT^C
AcyI 208 GACGTC G[GA]^CG[CT]C
AflIII 392 ACGTGT A^C[GA][CT]GT
AvaI 511 CTCGGG C^[CT]CG[GA]G
BalI 632 TGGCCA TGG^CCA
BanI 39 GGTGCC G^G[CT][GA]CC
BbrPI 391 CACGTG CAC^GTG
BbvCI 702 CCTCAGC CCTCAGC(-5/-2)
BsaAI 391 CACGTG [CT]AC^GT[GA]
BsaHI 208 GACGTC G[GA]^CG[CT]C
BsaWI 133 ACCGGA [AT]^CCGG[AT]
BsgI 238 CTGCAC GTGCAG(16/14)
BsmAI 732 GAGAC GTCTC(1/5)
BsmBI 732 GAGACG CGTCTC(1/5)
BsoBI 511 CTCGGG C^[CT]CG[GA]G
Bsp1407I 746 TGTACA T^GTACA
BsrFI 799 GCCGGC [GA]^CCGG[CT]
BsrGI 746 TGTACA T^GTACA
BsrI 621 CCAGT ACTGG(1/-1)
BstXI 536 CCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 799 GCCGGC [GA]^CCGG[CT]
EaeI 632 TGGCCA [CT]^GGCC[GA]
EcoT14I 44 CCAAGG C^C[AT][AT]GG
HgaI 438 GACGC GACGC(5/10)
Hin1I 208 GACGTC G[GA]^CG[CT]C
MluNI 632 TGGCCA TGG^CCA
MscI 632 TGGCCA TGG^CCA
NaeI 799 GCCGGC GCC^GGC
NgoMIV 799 GCCGGC G^CCGGC
NspI 441 GCATGT [GA]CATG^[CT]
PmaCI 391 CACGTG CAC^GTG
PmlI 391 CACGTG CAC^GTG
PvuII 853 CAGCTG CAG^CTG
SspBI 746 TGTACA T^GTACA
StuI 836 AGGCCT AGG^CCT
StyI 44 CCAAGG C^C[AT][AT]GG
TfiI 752 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 318 GATCC GGATC(4/5)
849 GATCC
AlwNI 739 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
827 CAGCCTCTG
AspHI 127 GAGCTC G[AT]GC[AT]^C
163 GAGCTC
BanII 127 GAGCTC G[GA]GC[CT]^C
163 GAGCTC
BcnI 71 CCGGG CC^[GC]GG
379 CCCGG
BglI 659 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
829 GCCTCTGAGGC
BsiHKAI 127 GAGCTC G[AT]GC[AT]^C
163 GAGCTC
BstYI 317 AGATCC [GA]^GATC[CT]
848 AGATCC
DdeI 703 CTCAG C^T[ACGT]AG
833 CTGAG
DraIII 286 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
401 CACACAGTG
DsaI 492 CCACGG C^C[GA][CT]GG
578 CCACGG
EcoT22I 816 ATGCAT ATGCA^T
865 ATGCAT
FauI 321 CCCGC CCCGC(4/6)
782 GCGGG
MflI 317 AGATCC [GA]^GATC[CT]
848 AGATCC
NciI 71 CCGGG CC^[GC]GG
379 CCCGG
NsiI 816 ATGCAT ATGCA^T
865 ATGCAT
PleI 527 GACTC GAGTC(4/5)
811 GACTC
PshAI 208 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
438 GACGCATGTC
SacI 127 GAGCTC GAGCT^C
163 GAGCTC
TspRI 241 CACTG CA[GC]TG[ACGT][ACGT]^
405 CAGTG
XhoII 317 AGATCC [GA]^GATC[CT]
848 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TthHB8I