Restriction map for
pF1KA1270
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BcgI BclI BfrI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI ClaI CpoI DraI DrdI
Eam1105I EarI EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI Ksp632I MluI MroI
NaeI NarI NdeI NgoMIV NotI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfoI SgrAI SmlI SpeI
SphI Sse8387I SwaI TfiI XbaI
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 1125 GGTACC G^GTACC
AccI 1614 GTATAC GT^[AC][GT]AC
AflIII 300 ACGTGT A^C[GA][CT]GT
AocI 487 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1658 AGCGCT AGC^GCT
Asp700I 237 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1125 GGTACC G^GTACC
AspHI 2734 GTGCTC G[AT]GC[AT]^C
AspI 2656 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1113 CCTAGG C^CTAGG
BciVI 639 GGATAC GTATCC(6/5)
BglII 1088 AGATCT A^GATCT
BlnI 1113 CCTAGG C^CTAGG
BsaAI 2264 TACGTA [CT]AC^GT[GA]
BsaBI 1153 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2890 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2734 GTGCTC G[AT]GC[AT]^C
Bsp1407I 2081 TGTACA T^GTACA
BspMI 1907 ACCTGC ACCTGC(4/8)
BsrFI 167 ACCGGC [GA]^CCGG[CT]
BsrGI 2081 TGTACA T^GTACA
Bst1107I 1614 GTATAC GTA^TAC
BstAPI 768 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 47 TTCGAA TT^CGAA
BstEII 520 GGTGACC G^GT[ACGT]ACC
BstPI 520 GGTGACC G^GT[ACGT]ACC
BstZ17I 1614 GTATAC GTA^TAC
Bsu36I 487 CCTGAGG CC^T[ACGT]AGG
Cfr10I 167 ACCGGC [GA]^CCGG[CT]
Eco47III 1658 AGCGCT AGC^GCT
Eco81I 487 CCTGAGG CC^T[ACGT]AGG
EcoO65I 520 GGTGACC G^GT[ACGT]ACC
EcoT22I 2195 ATGCAT ATGCA^T
KpnI 1125 GGTACC GGTAC^C
KspI 198 CCGCGG CCGC^GG
MamI 1153 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 405 CAATTG C^AATTG
MunI 405 CAATTG C^AATTG
NheI 589 GCTAGC G^CTAGC
NruI 2869 TCGCGA TCG^CGA
NsiI 2195 ATGCAT ATGCA^T
NspV 47 TTCGAA TT^CGAA
PflFI 2656 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 468 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 198 CCGCGG CCGC^GG
SfuI 47 TTCGAA TT^CGAA
SmaI 1772 CCCGGG CCC^GGG
SnaBI 2264 TACGTA TAC^GTA
SspBI 2081 TGTACA T^GTACA
SspI 425 AATATT AAT^ATT
Tsp509I 406 AATT ^AATT
Tth111I 2656 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 527 CCAAGGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1772 CCCGGG C^CCGGG
XmaI 1772 CCCGGG C^CCGGG
XmnI 237 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 1012 GGCGTC G[GA]^CG[CT]C
1871 GGCGTC
AvaI 1772 CCCGGG C^[CT]CG[GA]G
2366 CCCGAG
BbsI 730 GTCTTC GAAGAC(2/6)
1015 GTCTTC
BbvCI 72 CCTCAGC CCTCAGC(-5/-2)
2943 CCTCAGC
BlpI 570 GCTGAGC GC^T[ACGT]AGC
1450 GCTGAGC
Bpu1102I 570 GCTGAGC GC^T[ACGT]AGC
1450 GCTGAGC
BpuAI 730 GTCTTC GAAGAC(2/6)
1015 GTCTTC
BsaHI 1012 GGCGTC G[GA]^CG[CT]C
1871 GGCGTC
BsiEI 82 CGGCCG CG[GA][CT]^CG
119 CGGCCG
BsoBI 1772 CCCGGG C^[CT]CG[GA]G
2366 CCCGAG
CelII 570 GCTGAGC GC^T[ACGT]AGC
1450 GCTGAGC
DraIII 989 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1322 CACTGTGTG
EagI 82 CGGCCG C^GGCCG
119 CGGCCG
EclXI 82 CGGCCG C^GGCCG
119 CGGCCG
Eco52I 82 CGGCCG C^GGCCG
119 CGGCCG
EcoNI 1800 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2385 CCTGGCCCAGG
Hin1I 1012 GGCGTC G[GA]^CG[CT]C
1871 GGCGTC
MseI 431 TTAA T^TAA
1093 TTAA
NspI 786 GCATGT [GA]CATG^[CT]
1854 GCATGT
SfiI 120 GGCCGTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2355 GGCCCAGCAGGCC
Tru9I 431 TTAA T^TAA
1093 TTAA
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaII BalI BamHI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII