Restriction map for
pF1KA1534
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AviII AvrII BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco52I EcoRV
FbaI FseI FspI HindIII HpaI
Ksp632I KspI MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1091 GGTACC G^GTACC
AccI 67 GTCGAC GT^[AC][GT]AC
AcsI 1501 GAATTC [GA]^AATT[CT]
AflIII 1613 ACGTGT A^C[GA][CT]GT
AocI 721 CCTGAGG CC^T[ACGT]AGG
Aor51HI 355 AGCGCT AGC^GCT
ApoI 1501 GAATTC [GA]^AATT[CT]
Asp700I 1479 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1091 GGTACC G^GTACC
BalI 1596 TGGCCA TGG^CCA
BbeI 23 GGCGCC GGCGC^C
BfaI 530 CTAG C^TAG
BglII 1547 AGATCT A^GATCT
BsaWI 1262 ACCGGA [AT]^CCGG[AT]
BsmBI 566 GAGACG CGTCTC(1/5)
BsmI 691 GCATTC GAATGC(1/-1)
BssHII 333 GCGCGC G^CGCGC
Bsu36I 721 CCTGAGG CC^T[ACGT]AGG
DraIII 1699 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 355 AGCGCT AGC^GCT
Eco81I 721 CCTGAGG CC^T[ACGT]AGG
EcoNI 2247 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1501 GAATTC G^AATTC
EcoT14I 1118 CCAAGG C^C[AT][AT]GG
EcoT22I 689 ATGCAT ATGCA^T
HincII 67 GTCGAC GT[CT]^[GA]AC
HindII 67 GTCGAC GT[CT]^[GA]AC
KasI 23 GGCGCC G^GCGCC
KpnI 1091 GGTACC GGTAC^C
MaeI 530 CTAG C^TAG
MluNI 1596 TGGCCA TGG^CCA
MscI 1596 TGGCCA TGG^CCA
MseI 2417 TTAA T^TAA
NarI 23 GGCGCC GG^CGCC
NsiI 689 ATGCAT ATGCA^T
SalI 67 GTCGAC G^TCGAC
SfoI 23 GGCGCC GGC^GCC
StyI 1118 CCAAGG C^C[AT][AT]GG
Tru9I 2417 TTAA T^TAA
Tsp509I 1502 AATT ^AATT
XmnI 1479 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspI 879 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2182 GACTTTGTC
AvaI 1551 CTCGGG C^[CT]CG[GA]G
1662 CCCGAG
BamHI 1979 GGATCC G^GATCC
2131 GGATCC
BbsI 759 GAAGAC GAAGAC(2/6)
672 GTCTTC
BglI 395 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1589 GCCTCAGTGGC
BlpI 351 GCTCAGC GC^T[ACGT]AGC
2313 GCTGAGC
Bpu1102I 351 GCTCAGC GC^T[ACGT]AGC
2313 GCTGAGC
BpuAI 759 GAAGAC GAAGAC(2/6)
672 GTCTTC
BsoBI 1551 CTCGGG C^[CT]CG[GA]G
1662 CCCGAG
BsrFI 216 ACCGGC [GA]^CCGG[CT]
317 ACCGGC
BssSI 2050 CACGAG CACGAG(-5/-1)
2251 CACGAG
BstEII 276 GGTCACC G^GT[ACGT]ACC
1458 GGTCACC
BstPI 276 GGTCACC G^GT[ACGT]ACC
1458 GGTCACC
BstXI 818 CCACCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2021 CCACCTTCCTGG
CelII 351 GCTCAGC GC^T[ACGT]AGC
2313 GCTGAGC
Cfr10I 216 ACCGGC [GA]^CCGG[CT]
317 ACCGGC
EcoO65I 276 GGTCACC G^GT[ACGT]ACC
1458 GGTCACC
MslI 204 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
819 CACCTATGTG
PflFI 879 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2182 GACTTTGTC
PpuMI 580 GGGACCT [GA]G^G[AT]CC[CT]
1940 GGGACCC
PstI 2239 CTGCAG CTGCA^G
2359 CTGCAG
SmlI 295 CTCAAG C^T[CT][GA]AG
1510 CTCAAG
StuI 11 AGGCCT AGG^CCT
2352 AGGCCT
TaqI 68 TCGA T^CGA
1466 TCGA
TfiI 1124 GAATC G^A[AT]TC
1418 GAATC
Tth111I 879 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2182 GACTTTGTC
TthHB8I 68 TCGA T^CGA
1466 TCGA
XcmI 965 CCACGTTCGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1037 CCAGGGCTGCGGTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BanI BanII BbvCI BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI XhoII