Restriction map for
pF1KA1567
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BsaAI
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BssHII BssSI Bst1107I
BstUI BstXI BstZ17I ClaI CpoI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1109 GTAGAC GT^[AC][GT]AC
Alw44I 2382 GTGCAC G^TGCAC
AocI 2521 CCTCAGG CC^T[ACGT]AGG
ApaLI 2382 GTGCAC G^TGCAC
AvaI 991 CTCGAG C^[CT]CG[GA]G
BamHI 2517 GGATCC G^GATCC
BbsI 1635 GAAGAC GAAGAC(2/6)
BclI 1061 TGATCA T^GATCA
BlpI 649 GCTCAGC GC^T[ACGT]AGC
BpmI 2341 CTCCAG CTGGAG(16/14)
Bpu1102I 649 GCTCAGC GC^T[ACGT]AGC
BpuAI 1635 GAAGAC GAAGAC(2/6)
BseRI 1900 CTCCTC GAGGAG(10/8)
BsiEI 2511 CGGCCG CG[GA][CT]^CG
BsmI 1421 GAATGC GAATGC(1/-1)
BsoBI 991 CTCGAG C^[CT]CG[GA]G
BspHI 2469 TCATGA T^CATGA
BspLU11I 266 ACATGT A^CATGT
BspMI 591 ACCTGC ACCTGC(4/8)
BsrBI 1760 GAGCGG CCGCTC(-3/-3)
BstBI 2066 TTCGAA TT^CGAA
BstEII 774 GGTGACC G^GT[ACGT]ACC
BstPI 774 GGTGACC G^GT[ACGT]ACC
Bsu36I 2521 CCTCAGG CC^T[ACGT]AGG
CelII 649 GCTCAGC GC^T[ACGT]AGC
CfoI 7 GCGC GCG^C
DraI 352 TTTAAA TTT^AAA
DsaI 2007 CCGTGG C^C[GA][CT]GG
EagI 2511 CGGCCG C^GGCCG
EclXI 2511 CGGCCG C^GGCCG
Eco52I 2511 CGGCCG C^GGCCG
Eco81I 2521 CCTCAGG CC^T[ACGT]AGG
EcoO65I 774 GGTGACC G^GT[ACGT]ACC
EcoRI 2270 GAATTC G^AATTC
FbaI 1061 TGATCA T^GATCA
HaeII 6 GGCGCT [GA]GCGC^[CT]
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HincII 178 GTCAAC GT[CT]^[GA]AC
HindII 178 GTCAAC GT[CT]^[GA]AC
HindIII 404 AAGCTT A^AGCTT
MaeII 1575 ACGT A^CGT
MfeI 290 CAATTG C^AATTG
MunI 290 CAATTG C^AATTG
NheI 28 GCTAGC G^CTAGC
NspV 2066 TTCGAA TT^CGAA
PaeR7I 991 CTCGAG C^TCGAG
PflMI 537 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2330 CTGCAG CTGCA^G
RcaI 2469 TCATGA T^CATGA
SapI 1119 GAAGAGC GCTCTTC(1/4)
SexAI 2300 ACCAGGT A^CC[AT]GGT
SfuI 2066 TTCGAA TT^CGAA
SmlI 991 CTCGAG C^T[CT][GA]AG
StuI 2525 AGGCCT AGG^CCT
Van91I 537 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1805 CCATTGCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 991 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 266 ACATGT A^C[GA][CT]GT
1575 ACGTGT
BglII 1078 AGATCT A^GATCT
1886 AGATCT
BsaBI 127 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2461 GATAAAGATC
BsaI 588 GAGACC GGTCTC(1/5)
760 GGTCTC
Bsp1407I 269 TGTACA T^GTACA
886 TGTACA
BsrDI 1806 CATTGC GCAATG(2/0)
2189 GCAATG
BsrFI 1844 GCCGGT [GA]^CCGG[CT]
2099 ACCGGC
BsrGI 269 TGTACA T^GTACA
886 TGTACA
Cfr10I 1844 GCCGGT [GA]^CCGG[CT]
2099 ACCGGC
EcoT14I 60 CCTTGG C^C[AT][AT]GG
2528 CCTTGG
MamI 127 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2461 GATAAAGATC
MspA1I 1393 CAGCTG C[AC]G^C[GT]G
2359 CAGCTG
PpuMI 12 GGGACCC [GA]G^G[AT]CC[CT]
1925 AGGACCT
PvuII 1393 CAGCTG CAG^CTG
2359 CAGCTG
SacI 1195 GAGCTC GAGCT^C
1897 GAGCTC
SspBI 269 TGTACA T^GTACA
886 TGTACA
SspI 225 AATATT AAT^ATT
899 AATATT
StyI 60 CCTTGG C^C[AT][AT]GG
2528 CCTTGG
TfiI 114 GATTC G^A[AT]TC
1956 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII