Restriction map for
pF1KA1667
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AvaI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1932 CGCG CG^CG
AcyI 2062 GGCGCC G[GA]^CG[CT]C
AflIII 1170 ACATGT A^C[GA][CT]GT
AhdI 593 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 551 AGCGCT AGC^GCT
AspEI 593 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 798 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2062 GGCGCC GGCGC^C
BbsI 1330 GAAGAC GAAGAC(2/6)
BcgI 1604 GCATGGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 783 CCCGG CC^[GC]GG
BlpI 1532 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1532 GCTCAGC GC^T[ACGT]AGC
BpuAI 1330 GAAGAC GAAGAC(2/6)
BsaHI 2062 GGCGCC G[GA]^CG[CT]C
BsaI 803 GGTCTC GGTCTC(1/5)
BsgI 1021 CTGCAC GTGCAG(16/14)
BsiEI 1821 CGACCG CG[GA][CT]^CG
BsmBI 1762 GAGACG CGTCTC(1/5)
BspLU11I 1170 ACATGT A^CATGT
BsrBI 707 GAGCGG CCGCTC(-3/-3)
BsrDI 713 CATTGC GCAATG(2/0)
BstAPI 2091 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1932 CGCG CG^CG
BstXI 1226 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1532 GCTCAGC GC^T[ACGT]AGC
Eam1105I 593 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 551 AGCGCT AGC^GCT
EcoT22I 1906 ATGCAT ATGCA^T
Hin1I 2062 GGCGCC G[GA]^CG[CT]C
KasI 2062 GGCGCC G^GCGCC
MseI 347 TTAA T^TAA
MslI 1965 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1932 CGCG CG^CG
NarI 2062 GGCGCC GG^CGCC
NciI 783 CCCGG CC^[GC]GG
NcoI 908 CCATGG C^CATGG
NsiI 1906 ATGCAT ATGCA^T
NspI 1170 ACATGT [GA]CATG^[CT]
PflFI 798 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 2062 GGCGCC GGC^GCC
SpeI 1077 ACTAGT A^CTAGT
StuI 1464 AGGCCT AGG^CCT
Tru9I 347 TTAA T^TAA
Tsp45I 748 GTGAC ^GT[GC]AC
Tth111I 798 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 434 AAATTC [GA]^AATT[CT]
1915 GAATTT
AfaI 1713 GTAC GT^AC
1974 GTAC
ApoI 434 AAATTC [GA]^AATT[CT]
1915 GAATTT
BanI 2019 GGCACC G^G[CT][GA]CC
2062 GGCGCC
BbvCI 1053 CCTCAGC CCTCAGC(-5/-2)
1669 GCTGAGG
BglI 1960 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2072 GCCTCTCCGGC
BsmFI 416 GGGAC GGGAC(10/14)
1659 GTCCC
BsrI 892 ACTGG ACTGG(1/-1)
449 CCAGT
BstYI 927 AGATCC [GA]^GATC[CT]
1422 AGATCC
DraIII 408 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2107 CACGGGGTG
HgaI 1764 GACGC GACGC(5/10)
1640 GCGTC
HindIII 947 AAGCTT A^AGCTT
1444 AAGCTT
MaeIII 113 GTTAC ^GT[ACGT]AC
748 GTGAC
MflI 927 AGATCC [GA]^GATC[CT]
1422 AGATCC
PpuMI 635 AGGTCCT [GA]G^G[AT]CC[CT]
1390 AGGACCC
RsaI 1713 GTAC GT^AC
1974 GTAC
SacI 295 GAGCTC GAGCT^C
2034 GAGCTC
XhoII 927 AGATCC [GA]^GATC[CT]
1422 AGATCC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AspHI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspMI BssKI BssSI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I Van91I XcmI