Restriction map for
pF1KA1746
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccI AccII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AviII BbeI BbrPI BcgI
BclI BfrI BsiEI BsiWI BsmBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI FbaI FseI
FspI HpaI KasI KspI MluI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 2719 GACGTC GACGT^C
Acc65I 1314 GGTACC G^GTACC
AccIII 375 TCCGGA T^CCGGA
AflIII 3060 ACATGT A^C[GA][CT]GT
AocI 2886 CCTGAGG CC^T[ACGT]AGG
ApaI 887 GGGCCC GGGCC^C
Asp718I 1314 GGTACC G^GTACC
AspI 2845 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 367 CCTAGG C^CTAGG
BamHI 189 GGATCC G^GATCC
BbvCI 1018 GCTGAGG CCTCAGC(-5/-2)
BglII 2310 AGATCT A^GATCT
BlnI 367 CCTAGG C^CTAGG
BsaBI 1644 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 375 TCCGGA T^CCGGA
BsgI 855 CTGCAC GTGCAG(16/14)
BsmI 1759 GAATGC GAATGC(1/-1)
Bsp1407I 584 TGTACA T^GTACA
BspDI 1274 ATCGAT AT^CGAT
BspEI 375 TCCGGA T^CCGGA
BspLU11I 3060 ACATGT A^CATGT
BsrBI 1912 GAGCGG CCGCTC(-3/-3)
BsrDI 2015 CATTGC GCAATG(2/0)
BsrGI 584 TGTACA T^GTACA
BstAPI 2157 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2886 CCTGAGG CC^T[ACGT]AGG
CfoI 3116 GCGC GCG^C
ClaI 1274 ATCGAT AT^CGAT
DraI 147 TTTAAA TTT^AAA
DraIII 3151 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2886 CCTGAGG CC^T[ACGT]AGG
EcoNI 2882 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 664 GATATC GAT^ATC
EcoT22I 1356 ATGCAT ATGCA^T
HaeII 3115 AGCGCC [GA]GCGC^[CT]
HhaI 3116 GCGC GCG^C
HinP1I 3116 GCGC G^CGC
HincII 1803 GTTGAC GT[CT]^[GA]AC
HindII 1803 GTTGAC GT[CT]^[GA]AC
KpnI 1314 GGTACC GGTAC^C
MamI 1644 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1547 CAATTG C^AATTG
MroI 375 TCCGGA T^CCGGA
MunI 1547 CAATTG C^AATTG
NsiI 1356 ATGCAT ATGCA^T
PflFI 2845 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfiI 6 GGCCCGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 87 CCCGGG CCC^GGG
SspBI 584 TGTACA T^GTACA
StuI 2421 AGGCCT AGG^CCT
Tth111I 2845 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2087 CCATGGGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 520 GAATTT [GA]^AATT[CT]
2549 AAATTC
AcyI 905 GGCGTC G[GA]^CG[CT]C
2719 GACGTC
AhdI 39 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1738 GACTTGCTGTC
ApoI 520 GAATTT [GA]^AATT[CT]
2549 AAATTC
Asp700I 730 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3228 GAAAAACTTC
AspEI 39 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1738 GACTTGCTGTC
AspHI 2055 GAGCAC G[AT]GC[AT]^C
2912 GTGCTC
BanI 526 GGTGCC G^G[CT][GA]CC
1314 GGTACC
BciVI 1702 GTATCC GTATCC(6/5)
2182 GTATCC
BsaAI 1381 TACGTG [CT]AC^GT[GA]
3151 CACGTA
BsaHI 905 GGCGTC G[GA]^CG[CT]C
2719 GACGTC
BsaWI 375 TCCGGA [AT]^CCGG[AT]
2202 TCCGGT
BsiHKAI 2055 GAGCAC G[AT]GC[AT]^C
2912 GTGCTC
BspHI 2349 TCATGA T^CATGA
3224 TCATGA
Eam1105I 39 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1738 GACTTGCTGTC
FauI 26 GCGGG CCCGC(4/6)
994 GCGGG
HgaI 2073 GACGC GACGC(5/10)
906 GCGTC
Hin1I 905 GGCGTC G[GA]^CG[CT]C
2719 GACGTC
HindIII 2632 AAGCTT A^AGCTT
3184 AAGCTT
NcoI 2087 CCATGG C^CATGG
2250 CCATGG
PflMI 1128 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2955 CCAGAGGCTGG
RcaI 2349 TCATGA T^CATGA
3224 TCATGA
SapI 2329 GAAGAGC GCTCTTC(1/4)
3208 GAAGAGC
TaqI 576 TCGA T^CGA
1275 TCGA
TthHB8I 576 TCGA T^CGA
1275 TCGA
Van91I 1128 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2955 CCAGAGGCTGG
XmnI 730 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3228 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII