Restriction map for
pF1KA1775
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaWI
BseAI BseRI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI BstBI BstUI CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MfeI MluI
MroI MunI MvnI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 160 GTATAC GT^[AC][GT]AC
AflIII 1853 ACGTGT A^C[GA][CT]GT
Alw44I 2557 GTGCAC G^TGCAC
AocI 184 CCTGAGG CC^T[ACGT]AGG
ApaLI 2557 GTGCAC G^TGCAC
AseI 883 ATTAAT AT^TAAT
AsnI 883 ATTAAT AT^TAAT
AspI 1399 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1491 GGATCC G^GATCC
BglII 1883 AGATCT A^GATCT
BsaAI 157 CACGTA [CT]AC^GT[GA]
BsaBI 2019 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2439 CGTCTC CGTCTC(1/5)
Bsp1407I 957 TGTACA T^GTACA
BsrGI 957 TGTACA T^GTACA
Bst1107I 160 GTATAC GTA^TAC
BstAPI 522 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 372 GGTGACC G^GT[ACGT]ACC
BstPI 372 GGTGACC G^GT[ACGT]ACC
BstZ17I 160 GTATAC GTA^TAC
Bsu36I 184 CCTGAGG CC^T[ACGT]AGG
DrdI 85 GACAACGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 184 CCTGAGG CC^T[ACGT]AGG
EcoNI 129 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 372 GGTGACC G^GT[ACGT]ACC
MamI 2019 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2116 GCCGGC GCC^GGC
NgoMIV 2116 GCCGGC G^CCGGC
NheI 1600 GCTAGC G^CTAGC
NspI 954 GCATGT [GA]CATG^[CT]
PflFI 1399 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 883 ATTAAT AT^TAAT
PstI 520 CTGCAG CTGCA^G
SapI 280 GAAGAGC GCTCTTC(1/4)
SexAI 1814 ACCTGGT A^CC[AT]GGT
SfiI 18 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 957 TGTACA T^GTACA
SspI 1523 AATATT AAT^ATT
Tth111I 1399 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 393 GGCGCC G[GA]^CG[CT]C
2400 GGCGCC
AvaI 433 CCCGAG C^[CT]CG[GA]G
2364 CCCGAG
BalI 2126 TGGCCA TGG^CCA
2138 TGGCCA
BbeI 393 GGCGCC GGCGC^C
2400 GGCGCC
BbsI 1746 GAAGAC GAAGAC(2/6)
733 GTCTTC
BbvCI 1612 GCTGAGG CCTCAGC(-5/-2)
1678 GCTGAGG
BglI 19 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2202 GCCAATGCGGC
BpuAI 1746 GAAGAC GAAGAC(2/6)
733 GTCTTC
BsaHI 393 GGCGCC G[GA]^CG[CT]C
2400 GGCGCC
BsoBI 433 CCCGAG C^[CT]CG[GA]G
2364 CCCGAG
BspMI 444 ACCTGC ACCTGC(4/8)
527 ACCTGC
BsrBI 1905 CCGCTC CCGCTC(-3/-3)
1068 GAGCGG
BsrFI 11 GCCGGT [GA]^CCGG[CT]
2116 GCCGGC
Cfr10I 11 GCCGGT [GA]^CCGG[CT]
2116 GCCGGC
EcoRI 839 GAATTC G^AATTC
1896 GAATTC
HgaI 1609 GACGC GACGC(5/10)
236 GCGTC
Hin1I 393 GGCGCC G[GA]^CG[CT]C
2400 GGCGCC
HincII 247 GTTGAC GT[CT]^[GA]AC
2511 GTCAAC
HindII 247 GTTGAC GT[CT]^[GA]AC
2511 GTCAAC
KasI 393 GGCGCC G^GCGCC
2400 GGCGCC
MluNI 2126 TGGCCA TGG^CCA
2138 TGGCCA
MscI 2126 TGGCCA TGG^CCA
2138 TGGCCA
NarI 393 GGCGCC GG^CGCC
2400 GGCGCC
PleI 1156 GACTC GAGTC(4/5)
1417 GACTC
SacI 2369 GAGCTC GAGCT^C
2527 GAGCTC
SfoI 393 GGCGCC GGC^GCC
2400 GGCGCC
TfiI 342 GAATC G^A[AT]TC
1440 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII