Restriction map for
pF1KA1779
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AclI AflII AhdI
ApaI AscI AseI AsnI AspEI
AviII AvrII BbeI BbvCI BclI
BfrI BglI BglII BlnI BsaBI
BsaI BsiWI BspLU11I BssHII BssSI
Bst1107I BstAPI BstXI BstZ17I CpoI
Eam1105I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KasI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SwaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 3387 GGTACC G^GTACC
AccIII 686 TCCGGA T^CCGGA
AcyI 486 GGCGTC G[GA]^CG[CT]C
AgeI 2932 ACCGGT A^CCGGT
Asp718I 3387 GGTACC G^GTACC
BalI 353 TGGCCA TGG^CCA
BcgI 1122 GCATAAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2556 GGATAC GTATCC(6/5)
BsaHI 486 GGCGTC G[GA]^CG[CT]C
BseAI 686 TCCGGA T^CCGGA
BsmI 3368 GCATTC GAATGC(1/-1)
BspDI 918 ATCGAT AT^CGAT
BspEI 686 TCCGGA T^CCGGA
BspHI 3374 TCATGA T^CATGA
BsrBI 73 GAGCGG CCGCTC(-3/-3)
BsrDI 2680 GCAATG GCAATG(2/0)
BstBI 1130 TTCGAA TT^CGAA
ClaI 918 ATCGAT AT^CGAT
DraI 2029 TTTAAA TTT^AAA
DraIII 1342 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2584 GACCCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2216 CGGCCG C^GGCCG
EclXI 2216 CGGCCG C^GGCCG
Eco52I 2216 CGGCCG C^GGCCG
EcoNI 894 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2658 GATATC GAT^ATC
Hin1I 486 GGCGTC G[GA]^CG[CT]C
HincII 502 GTTGAC GT[CT]^[GA]AC
HindII 502 GTTGAC GT[CT]^[GA]AC
KpnI 3387 GGTACC GGTAC^C
MluNI 353 TGGCCA TGG^CCA
MroI 686 TCCGGA T^CCGGA
MscI 353 TGGCCA TGG^CCA
NaeI 2218 GCCGGC GCC^GGC
NgoMIV 2218 GCCGGC G^CCGGC
NspV 1130 TTCGAA TT^CGAA
PaeR7I 2364 CTCGAG C^TCGAG
PflMI 1822 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2932 ACCGGT A^CCGGT
PpuMI 902 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 3374 TCATGA T^CATGA
SexAI 3383 ACCAGGT A^CC[AT]GGT
SfuI 1130 TTCGAA TT^CGAA
SmaI 25 CCCGGG CCC^GGG
SpeI 3241 ACTAGT A^CTAGT
SspI 2171 AATATT AAT^ATT
StuI 2490 AGGCCT AGG^CCT
Van91I 1822 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 228 TCTAGA T^CTAGA
XcmI 3462 CCAGTTTTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2364 CTCGAG C^TCGAG
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 2034 ACACGT A^C[GA][CT]GT
2864 ACACGT
Alw44I 433 GTGCAC G^TGCAC
2936 GTGCAC
AocI 316 CCTCAGG CC^T[ACGT]AGG
898 CCTCAGG
Aor51HI 2989 AGCGCT AGC^GCT
3043 AGCGCT
ApaLI 433 GTGCAC G^TGCAC
2936 GTGCAC
AspI 1645 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2208 GACGCGGTC
BamHI 638 GGATCC G^GATCC
800 GGATCC
BlpI 556 GCTCAGC GC^T[ACGT]AGC
1884 GCTGAGC
Bpu1102I 556 GCTCAGC GC^T[ACGT]AGC
1884 GCTGAGC
BsiEI 2212 CGGTCG CG[GA][CT]^CG
2216 CGGCCG
Bsu36I 316 CCTCAGG CC^T[ACGT]AGG
898 CCTCAGG
CelII 556 GCTCAGC GC^T[ACGT]AGC
1884 GCTGAGC
Eco47III 2989 AGCGCT AGC^GCT
3043 AGCGCT
Eco81I 316 CCTCAGG CC^T[ACGT]AGG
898 CCTCAGG
EcoT14I 2390 CCATGG C^C[AT][AT]GG
3107 CCATGG
HaeII 2989 AGCGCT [GA]GCGC^[CT]
3043 AGCGCT
KspI 6 CCGCGG CCGC^GG
2342 CCGCGG
MslI 1875 CACGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3369 CATTCTCATG
NcoI 2390 CCATGG C^CATGG
3107 CCATGG
NspI 332 GCATGT [GA]CATG^[CT]
1997 ACATGC
PflFI 1645 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2208 GACGCGGTC
SacI 554 GAGCTC GAGCT^C
1856 GAGCTC
SacII 6 CCGCGG CCGC^GG
2342 CCGCGG
StyI 2390 CCATGG C^C[AT][AT]GG
3107 CCATGG
Tth111I 1645 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2208 GACGCGGTC
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PmaCI PmlI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI