Restriction map for
pF1KA1824
##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I AviII AvrII
BamHI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BspEI BsrFI BssHII
BssSI Bst1107I BstAPI BstUI BstZ17I
CelII Cfr10I CpoI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2278 GGTACC G^GTACC
AhdI 1372 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 934 GATCC GGATC(4/5)
AocI 750 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1355 AGCGCT AGC^GCT
ApaI 1450 GGGCCC GGGCC^C
Asp718I 2278 GGTACC G^GTACC
AspEI 1372 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2442 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1781 CCCGAG C^[CT]CG[GA]G
BbeI 1763 GGCGCC GGCGC^C
BbrPI 1503 CACGTG CAC^GTG
BbvCI 2365 GCTGAGG CCTCAGC(-5/-2)
BsaAI 1503 CACGTG [CT]AC^GT[GA]
BsgI 1336 CTGCAC GTGCAG(16/14)
BsiEI 1119 CGGTCG CG[GA][CT]^CG
BsmBI 105 GAGACG CGTCTC(1/5)
BsmI 1752 GAATGC GAATGC(1/-1)
BsoBI 1781 CCCGAG C^[CT]CG[GA]G
Bsp1407I 911 TGTACA T^GTACA
BspDI 995 ATCGAT AT^CGAT
BspHI 420 TCATGA T^CATGA
BspLU11I 914 ACATGT A^CATGT
BsrBI 1900 GAGCGG CCGCTC(-3/-3)
BsrGI 911 TGTACA T^GTACA
BstBI 650 TTCGAA TT^CGAA
Bsu36I 750 CCTGAGG CC^T[ACGT]AGG
ClaI 995 ATCGAT AT^CGAT
DraI 2197 TTTAAA TTT^AAA
Eam1105I 1372 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1355 AGCGCT AGC^GCT
Eco81I 750 CCTGAGG CC^T[ACGT]AGG
HindIII 1985 AAGCTT A^AGCTT
HpaI 1006 GTTAAC GTT^AAC
KasI 1763 GGCGCC G^GCGCC
KpnI 2278 GGTACC GGTAC^C
NarI 1763 GGCGCC GG^CGCC
NcoI 528 CCATGG C^CATGG
NspI 914 ACATGT [GA]CATG^[CT]
NspV 650 TTCGAA TT^CGAA
PflFI 2442 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1503 CACGTG CAC^GTG
PmlI 1503 CACGTG CAC^GTG
PvuII 283 CAGCTG CAG^CTG
RcaI 420 TCATGA T^CATGA
SacI 1290 GAGCTC GAGCT^C
SapI 1046 GCTCTTC GCTCTTC(1/4)
ScaI 1321 AGTACT AGT^ACT
SfiI 2743 GGCCACAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1763 GGCGCC GGC^GCC
SfuI 650 TTCGAA TT^CGAA
SspBI 911 TGTACA T^GTACA
Tth111I 2442 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1332 CCAACTGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BalI 608 TGGCCA TGG^CCA
2742 TGGCCA
BglII 1073 AGATCT A^GATCT
1632 AGATCT
BspMI 2165 ACCTGC ACCTGC(4/8)
943 GCAGGT
BstEII 1461 GGTCACC G^GT[ACGT]ACC
1652 GGTCACC
BstPI 1461 GGTCACC G^GT[ACGT]ACC
1652 GGTCACC
BstXI 599 CCAGGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2493 CCACAACAATGG
DsaI 528 CCATGG C^C[GA][CT]GG
1648 CCACGG
EcoO65I 1461 GGTCACC G^GT[ACGT]ACC
1652 GGTCACC
HincII 964 GTCAAC GT[CT]^[GA]AC
1006 GTTAAC
HindII 964 GTCAAC GT[CT]^[GA]AC
1006 GTTAAC
MaeII 410 ACGT A^CGT
1504 ACGT
MluNI 608 TGGCCA TGG^CCA
2742 TGGCCA
MscI 608 TGGCCA TGG^CCA
2742 TGGCCA
PflMI 198 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
738 CCAGCTCCTGG
Van91I 198 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
738 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII