Restriction map for
pF1KA1839
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstXI BstZ17I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRII
FbaI FseI FspI HaeIII HgaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvaI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tsp45I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccII 611 CGCG CG^CG
AflIII 1287 ACATGT A^C[GA][CT]GT
AlwNI 338 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 777 ATTAAT AT^TAAT
AsnI 777 ATTAAT AT^TAAT
Asp700I 141 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 45 GAGCTC G[AT]GC[AT]^C
AspI 367 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 95 GGCACC G^G[CT][GA]CC
BcnI 1243 CCGGG CC^[GC]GG
BlpI 106 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 106 GCTGAGC GC^T[ACGT]AGC
BsiEI 75 CGACCG CG[GA][CT]^CG
BsiHKAI 45 GAGCTC G[AT]GC[AT]^C
BsmFI 329 GTCCC GGGAC(10/14)
Bsp1407I 549 TGTACA T^GTACA
BspHI 1041 TCATGA T^CATGA
BspLU11I 1287 ACATGT A^CATGT
BsrDI 641 CATTGC GCAATG(2/0)
BsrFI 102 GCCGGC [GA]^CCGG[CT]
BsrGI 549 TGTACA T^GTACA
BsrI 904 CCAGT ACTGG(1/-1)
BssKI 1243 CCGGG ^CC[ACGT]GG
BssSI 42 CACGAG CACGAG(-5/-1)
BstUI 611 CGCG CG^CG
BstYI 1524 AGATCC [GA]^GATC[CT]
CelII 106 GCTGAGC GC^T[ACGT]AGC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
DraI 456 TTTAAA TTT^AAA
EarI 653 CTCTTC CTCTTC(1/4)
EcoNI 1528 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 964 GAATTC G^AATTC
EcoRV 27 GATATC GAT^ATC
EcoT22I 1067 ATGCAT ATGCA^T
HaeII 827 AGCGCC [GA]GCGC^[CT]
HincII 1330 GTTGAC GT[CT]^[GA]AC
HindII 1330 GTTGAC GT[CT]^[GA]AC
HindIII 1403 AAGCTT A^AGCTT
HphI 1246 GGTGA GGTGA(8/7)
Ksp632I 653 CTCTTC CTCTTC(1/4)
MaeII 1371 ACGT A^CGT
MflI 1524 AGATCC [GA]^GATC[CT]
MslI 872 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 611 CGCG CG^CG
NaeI 102 GCCGGC GCC^GGC
NciI 1243 CCGGG CC^[GC]GG
NgoMIV 102 GCCGGC G^CCGGC
NsiI 1067 ATGCAT ATGCA^T
PflFI 367 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 433 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 777 ATTAAT AT^TAAT
PvuII 989 CAGCTG CAG^CTG
RcaI 1041 TCATGA T^CATGA
SacI 45 GAGCTC GAGCT^C
ScaI 143 AGTACT AGT^ACT
ScrFI 1243 CCGGG CC^[ACGT]GG
SmlI 469 CTCAAG C^T[CT][GA]AG
SphI 645 GCATGC GCATG^C
SspBI 549 TGTACA T^GTACA
TfiI 727 GAATC G^A[AT]TC
Tth111I 367 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 433 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1100 CCAAACCTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1524 AGATCC [GA]^GATC[CT]
XmnI 141 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 1525 GATCC GGATC(4/5)
1355 GGATC
AocI 679 CCTGAGG CC^T[ACGT]AGG
1528 CCTAAGG
AvaI 10 CCCGAG C^[CT]CG[GA]G
68 CTCGGG
BanII 45 GAGCTC G[GA]GC[CT]^C
152 GGGCTC
BbsI 628 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BbvCI 670 CCTCAGC CCTCAGC(-5/-2)
118 GCTGAGG
BmyI 45 GAGCTC G[AGT]GC[ACT]^C
152 GGGCTC
BpuAI 628 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BsoBI 10 CCCGAG C^[CT]CG[GA]G
68 CTCGGG
Bsp1286I 45 GAGCTC G[AGT]GC[ACT]^C
152 GGGCTC
BspMI 98 ACCTGC ACCTGC(4/8)
1050 ACCTGC
Bsu36I 679 CCTGAGG CC^T[ACGT]AGG
1528 CCTAAGG
CfoI 610 GCGC GCG^C
828 GCGC
DsaI 438 CCATGG C^C[GA][CT]GG
1484 CCATGG
Eco81I 679 CCTGAGG CC^T[ACGT]AGG
1528 CCTAAGG
FauI 63 CCCGC CCCGC(4/6)
165 GCGGG
HapII 103 CCGG C^CGG
1243 CCGG
HhaI 610 GCGC GCG^C
828 GCGC
HinP1I 610 GCGC G^CGC
828 GCGC
HpaII 103 CCGG C^CGG
1243 CCGG
MaeIII 912 GTTAC ^GT[ACGT]AC
950 GTAAC
MspA1I 989 CAGCTG C[AC]G^C[GT]G
1351 CAGCGG
MspI 103 CCGG C^CGG
1243 CCGG
NcoI 438 CCATGG C^CATGG
1484 CCATGG
PleI 251 GACTC GAGTC(4/5)
1138 GACTC
SfcI 944 CTGTAG C^T[GA][CT]AG
1257 CTGTAG
TspRI 744 CACTG CA[GC]TG[ACGT][ACGT]^
905 CAGTG
XbaI 1168 TCTAGA T^CTAGA
1198 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BfaI BsaJI BseRI
BsiYI BslI BstF5I Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoT14I
Fnu4HI FokI HinfI ItaI MaeI
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV NspI PpuMI
RsaI Sau3AI Sau96I SfaNI StyI
TaqI Tru9I TseI Tsp509I TthHB8I