Restriction map for
pF1KB0047
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BbrPI
BfrI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI BspEI BspLU11I
BssHII Bst1107I BstBI BstZ17I CelII
CpoI DraIII Eco47III FseI HpaI
KpnI KspI MroI NdeI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII ScaI SexAI
SfuI SgrAI SpeI SwaI Tth111I
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 5678 GTCGAC GT^[AC][GT]AC
AclI 4669 AACGTT AA^CGTT
AflIII 5773 ACGCGT A^C[GA][CT]GT
AocI 631 CCTCAGG CC^T[ACGT]AGG
AviII 1161 TGCGCA TGC^GCA
BanI 5921 GGCGCC G^G[CT][GA]CC
BbeI 5921 GGCGCC GGCGC^C
BbvCI 5874 GCTGAGG CCTCAGC(-5/-2)
BcgI 3284 CGACCGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4047 GGATAC GTATCC(6/5)
BsaAI 3596 TACGTA [CT]AC^GT[GA]
BsaBI 2214 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 5929 CTGCAC GTGCAG(16/14)
BsmI 1642 GAATGC GAATGC(1/-1)
BspDI 1555 ATCGAT AT^CGAT
BstEII 2488 GGTTACC G^GT[ACGT]ACC
BstPI 2488 GGTTACC G^GT[ACGT]ACC
Bsu36I 631 CCTCAGG CC^T[ACGT]AGG
ClaI 1555 ATCGAT AT^CGAT
DraI 2442 TTTAAA TTT^AAA
EagI 1239 CGGCCG C^GGCCG
EclXI 1239 CGGCCG C^GGCCG
Eco52I 1239 CGGCCG C^GGCCG
Eco81I 631 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2488 GGTTACC G^GT[ACGT]ACC
EcoRI 2260 GAATTC G^AATTC
EcoRV 2455 GATATC GAT^ATC
FspI 1161 TGCGCA TGC^GCA
KasI 5921 GGCGCC G^GCGCC
MamI 2214 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5773 ACGCGT A^CGCGT
NaeI 5918 GCCGGC GCC^GGC
NarI 5921 GGCGCC GG^CGCC
NgoMIV 5918 GCCGGC G^CCGGC
NheI 5409 GCTAGC G^CTAGC
Psp1406I 4669 AACGTT AA^CGTT
SalI 5678 GTCGAC G^TCGAC
SbfI 3291 CCTGCAGG CCTGCA^GG
SfiI 2496 GGCCAGAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5921 GGCGCC GGC^GCC
SmaI 4559 CCCGGG CCC^GGG
SnaBI 3596 TACGTA TAC^GTA
SphI 491 GCATGC GCATG^C
Sse8387I 3291 CCTGCAGG CCTGCA^GG
XbaI 3756 TCTAGA T^CTAGA
XmaCI 4559 CCCGGG C^CCGGG
XmaI 4559 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 5288 GGCGTC G[GA]^CG[CT]C
5921 GGCGCC
AhdI 1368 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2869 GACTTGGAGTC
ApaI 4562 GGGCCC GGGCC^C
4697 GGGCCC
AspEI 1368 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2869 GACTTGGAGTC
BclI 1131 TGATCA T^GATCA
4874 TGATCA
BsaHI 5288 GGCGTC G[GA]^CG[CT]C
5921 GGCGCC
BsmBI 3280 GAGACG CGTCTC(1/5)
5998 GAGACG
Bsp1407I 422 TGTACA T^GTACA
2372 TGTACA
BsrFI 5870 ACCGGC [GA]^CCGG[CT]
5918 GCCGGC
BsrGI 422 TGTACA T^GTACA
2372 TGTACA
Cfr10I 5870 ACCGGC [GA]^CCGG[CT]
5918 GCCGGC
DrdI 49 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3865 GACGCGCAGGTC
Eam1105I 1368 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2869 GACTTGGAGTC
EcoNI 3819 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4365 CCTGCAGCAGG
EcoT22I 489 ATGCAT ATGCA^T
1581 ATGCAT
FbaI 1131 TGATCA T^GATCA
4874 TGATCA
Hin1I 5288 GGCGTC G[GA]^CG[CT]C
5921 GGCGCC
MfeI 4250 CAATTG C^AATTG
5164 CAATTG
MunI 4250 CAATTG C^AATTG
5164 CAATTG
NcoI 126 CCATGG C^CATGG
1016 CCATGG
NsiI 489 ATGCAT ATGCA^T
1581 ATGCAT
PflMI 4308 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5454 CCAGCAACTGG
SspBI 422 TGTACA T^GTACA
2372 TGTACA
StuI 2327 AGGCCT AGG^CCT
2504 AGGCCT
Van91I 4308 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5454 CCAGCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoI XhoII
XmnI