##################################
# NO CUT ENZYMES ( 28 enzymes) #
##################################
AatII Acc65I AscI AseI AsnI
Asp718I BbrPI CpoI DraI FseI
HpaI KpnI KspI NotI PacI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AclI 12901 AACGTT AA^CGTT
AflII 12106 CTTAAG C^TTAAG
AgeI 12227 ACCGGT A^CCGGT
BfrI 12106 CTTAAG C^TTAAG
BsiEI 9558 CGGCCG CG[GA][CT]^CG
BsiWI 7540 CGTACG C^GTACG
BspDI 10060 ATCGAT AT^CGAT
BssHII 1642 GCGCGC G^CGCGC
ClaI 10060 ATCGAT AT^CGAT
EagI 9558 CGGCCG C^GGCCG
EclXI 9558 CGGCCG C^GGCCG
Eco52I 9558 CGGCCG C^GGCCG
MluI 8640 ACGCGT A^CGCGT
NdeI 11065 CATATG CA^TATG
NheI 12531 GCTAGC G^CTAGC
PaeR7I 8779 CTCGAG C^TCGAG
PinAI 12227 ACCGGT A^CCGGT
Psp1406I 12901 AACGTT AA^CGTT
SalI 7177 GTCGAC G^TCGAC
SbfI 5410 CCTGCAGG CCTGCA^GG
ScaI 5303 AGTACT AGT^ACT
Sse8387I 5410 CCTGCAGG CCTGCA^GG
XbaI 9261 TCTAGA T^CTAGA
XhoI 8779 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 1651 GTGCAC G^TGCAC
1741 GTGCAC
Aor51HI 7802 AGCGCT AGC^GCT
11585 AGCGCT
ApaLI 1651 GTGCAC G^TGCAC
1741 GTGCAC
AviII 2003 TGCGCA TGC^GCA
10403 TGCGCA
AvrII 4371 CCTAGG C^CTAGG
6378 CCTAGG
BlnI 4371 CCTAGG C^CTAGG
6378 CCTAGG
BsaBI 11364 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
11749 GATCCTCATC
BsmBI 9564 CGTCTC CGTCTC(1/5)
1957 GAGACG
Eco47III 7802 AGCGCT AGC^GCT
11585 AGCGCT
EcoRI 2641 GAATTC G^AATTC
3502 GAATTC
EcoRV 8398 GATATC GAT^ATC
9550 GATATC
EcoT22I 852 ATGCAT ATGCA^T
7828 ATGCAT
FspI 2003 TGCGCA TGC^GCA
10403 TGCGCA
MamI 11364 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
11749 GATCCTCATC
MfeI 5774 CAATTG C^AATTG
12684 CAATTG
MunI 5774 CAATTG C^AATTG
12684 CAATTG
NruI 3104 TCGCGA TCG^CGA
6587 TCGCGA
NsiI 852 ATGCAT ATGCA^T
7828 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 188 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AhdI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspEI AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI NspV PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfiI
SfoI SfuI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI