Restriction map for pF1KB0497
##################################
# NO CUT ENZYMES (   28 enzymes) #
##################################

AatII       Acc65I      AscI        AseI        AsnI        
Asp718I     BbrPI       CpoI        DraI        FseI        
HpaI        KpnI        KspI        NotI        PacI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RsrII       SacII       SgrAI       SnaBI       
SpeI        SspI        SwaI        


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# ONE CUT ENZYMES (   24 enzymes) #
###################################

AclI        12901  AACGTT               AA^CGTT
AflII       12106  CTTAAG               C^TTAAG
AgeI        12227  ACCGGT               A^CCGGT
BfrI        12106  CTTAAG               C^TTAAG
BsiEI        9558  CGGCCG               CG[GA][CT]^CG
BsiWI        7540  CGTACG               C^GTACG
BspDI       10060  ATCGAT               AT^CGAT
BssHII       1642  GCGCGC               G^CGCGC
ClaI        10060  ATCGAT               AT^CGAT
EagI         9558  CGGCCG               C^GGCCG
EclXI        9558  CGGCCG               C^GGCCG
Eco52I       9558  CGGCCG               C^GGCCG
MluI         8640  ACGCGT               A^CGCGT
NdeI        11065  CATATG               CA^TATG
NheI        12531  GCTAGC               G^CTAGC
PaeR7I       8779  CTCGAG               C^TCGAG
PinAI       12227  ACCGGT               A^CCGGT
Psp1406I    12901  AACGTT               AA^CGTT
SalI         7177  GTCGAC               G^TCGAC
SbfI         5410  CCTGCAGG             CCTGCA^GG
ScaI         5303  AGTACT               AGT^ACT
Sse8387I     5410  CCTGCAGG             CCTGCA^GG
XbaI         9261  TCTAGA               T^CTAGA
XhoI         8779  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Alw44I       1651  GTGCAC               G^TGCAC
             1741  GTGCAC              
Aor51HI      7802  AGCGCT               AGC^GCT
            11585  AGCGCT              
ApaLI        1651  GTGCAC               G^TGCAC
             1741  GTGCAC              
AviII        2003  TGCGCA               TGC^GCA
            10403  TGCGCA              
AvrII        4371  CCTAGG               C^CTAGG
             6378  CCTAGG              
BlnI         4371  CCTAGG               C^CTAGG
             6378  CCTAGG              
BsaBI       11364  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
            11749  GATCCTCATC          
BsmBI        9564  CGTCTC               CGTCTC(1/5)
             1957  GAGACG              
Eco47III     7802  AGCGCT               AGC^GCT
            11585  AGCGCT              
EcoRI        2641  GAATTC               G^AATTC
             3502  GAATTC              
EcoRV        8398  GATATC               GAT^ATC
             9550  GATATC              
EcoT22I       852  ATGCAT               ATGCA^T
             7828  ATGCAT              
FspI         2003  TGCGCA               TGC^GCA
            10403  TGCGCA              
MamI        11364  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
            11749  GATCCTCATC          
MfeI         5774  CAATTG               C^AATTG
            12684  CAATTG              
MunI         5774  CAATTG               C^AATTG
            12684  CAATTG              
NruI         3104  TCGCGA               TCG^CGA
             6587  TCGCGA              
NsiI          852  ATGCAT               ATGCA^T
             7828  ATGCAT              


#########################################
# 3 OR MORE CUT ENZYMES (  188 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AflIII      AhdI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        Eam1105I    EarI        
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MroI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        NspV        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SmaI        SmlI        SphI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        XmnI        
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