################################## # NO CUT ENZYMES ( 28 enzymes) # ################################## AatII Acc65I AscI AseI AsnI Asp718I BbrPI CpoI DraI FseI HpaI KpnI KspI NotI PacI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SgrAI SnaBI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AclI 12901 AACGTT AA^CGTT AflII 12106 CTTAAG C^TTAAG AgeI 12227 ACCGGT A^CCGGT BfrI 12106 CTTAAG C^TTAAG BsiEI 9558 CGGCCG CG[GA][CT]^CG BsiWI 7540 CGTACG C^GTACG BspDI 10060 ATCGAT AT^CGAT BssHII 1642 GCGCGC G^CGCGC ClaI 10060 ATCGAT AT^CGAT EagI 9558 CGGCCG C^GGCCG EclXI 9558 CGGCCG C^GGCCG Eco52I 9558 CGGCCG C^GGCCG MluI 8640 ACGCGT A^CGCGT NdeI 11065 CATATG CA^TATG NheI 12531 GCTAGC G^CTAGC PaeR7I 8779 CTCGAG C^TCGAG PinAI 12227 ACCGGT A^CCGGT Psp1406I 12901 AACGTT AA^CGTT SalI 7177 GTCGAC G^TCGAC SbfI 5410 CCTGCAGG CCTGCA^GG ScaI 5303 AGTACT AGT^ACT Sse8387I 5410 CCTGCAGG CCTGCA^GG XbaI 9261 TCTAGA T^CTAGA XhoI 8779 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Alw44I 1651 GTGCAC G^TGCAC 1741 GTGCAC Aor51HI 7802 AGCGCT AGC^GCT 11585 AGCGCT ApaLI 1651 GTGCAC G^TGCAC 1741 GTGCAC AviII 2003 TGCGCA TGC^GCA 10403 TGCGCA AvrII 4371 CCTAGG C^CTAGG 6378 CCTAGG BlnI 4371 CCTAGG C^CTAGG 6378 CCTAGG BsaBI 11364 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 11749 GATCCTCATC BsmBI 9564 CGTCTC CGTCTC(1/5) 1957 GAGACG Eco47III 7802 AGCGCT AGC^GCT 11585 AGCGCT EcoRI 2641 GAATTC G^AATTC 3502 GAATTC EcoRV 8398 GATATC GAT^ATC 9550 GATATC EcoT22I 852 ATGCAT ATGCA^T 7828 ATGCAT FspI 2003 TGCGCA TGC^GCA 10403 TGCGCA MamI 11364 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 11749 GATCCTCATC MfeI 5774 CAATTG C^AATTG 12684 CAATTG MunI 5774 CAATTG C^AATTG 12684 CAATTG NruI 3104 TCGCGA TCG^CGA 6587 TCGCGA NsiI 852 ATGCAT ATGCA^T 7828 ATGCAT ######################################### # 3 OR MORE CUT ENZYMES ( 188 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspEI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI NspV PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SmaI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI