Restriction map for
pF1KB1891
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BstAPI BstUI
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwNI 1232 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1483 CTCGAG C^[CT]CG[GA]G
BalI 4166 TGGCCA TGG^CCA
BbvCI 3751 CCTCAGC CCTCAGC(-5/-2)
BcgI 4102 GCAGATAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 455 GGATAC GTATCC(6/5)
BcnI 2891 CCCGG CC^[GC]GG
BpmI 599 CTGGAG CTGGAG(16/14)
BsaAI 971 CACGTA [CT]AC^GT[GA]
BsgI 1303 GTGCAG GTGCAG(16/14)
BsmI 3657 GAATGC GAATGC(1/-1)
BsoBI 1483 CTCGAG C^[CT]CG[GA]G
BspHI 372 TCATGA T^CATGA
BssSI 2933 CACGAG CACGAG(-5/-1)
Bst1107I 2607 GTATAC GTA^TAC
BstBI 829 TTCGAA TT^CGAA
BstEII 2402 GGTCACC G^GT[ACGT]ACC
BstPI 2402 GGTCACC G^GT[ACGT]ACC
BstXI 1581 CCAGATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2607 GTATAC GTA^TAC
CfoI 4100 GCGC GCG^C
DraI 265 TTTAAA TTT^AAA
EaeI 4166 TGGCCA [CT]^GGCC[GA]
EcoNI 3786 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2402 GGTCACC G^GT[ACGT]ACC
EcoRI 3315 GAATTC G^AATTC
EcoT14I 4202 CCAAGG C^C[AT][AT]GG
EcoT22I 860 ATGCAT ATGCA^T
HhaI 4100 GCGC GCG^C
HinP1I 4100 GCGC G^CGC
HincII 3865 GTCAAC GT[CT]^[GA]AC
HindII 3865 GTCAAC GT[CT]^[GA]AC
MaeII 972 ACGT A^CGT
MluNI 4166 TGGCCA TGG^CCA
MscI 4166 TGGCCA TGG^CCA
NciI 2891 CCCGG CC^[GC]GG
NsiI 860 ATGCAT ATGCA^T
NspV 829 TTCGAA TT^CGAA
PaeR7I 1483 CTCGAG C^TCGAG
RcaI 372 TCATGA T^CATGA
SacI 1173 GAGCTC GAGCT^C
SfuI 829 TTCGAA TT^CGAA
StyI 4202 CCAAGG C^C[AT][AT]GG
XhoI 1483 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BsaI 363 GGTCTC GGTCTC(1/5)
2594 GGTCTC
BspLU11I 1654 ACATGT A^CATGT
1942 ACATGT
BspMI 939 ACCTGC ACCTGC(4/8)
3792 GCAGGT
DraII 2722 AGGTCCC [GA]G^G[ACGT]CC[CT]
2818 AGGTCCC
DsaI 3210 CCGTGG C^C[GA][CT]GG
3231 CCGTGG
EcoO109I 2722 AGGTCCC [GA]G^G[ACGT]CC[CT]
2818 AGGTCCC
EcoRV 1584 GATATC GAT^ATC
4195 GATATC
HapII 2526 CCGG C^CGG
2892 CCGG
HpaII 2526 CCGG C^CGG
2892 CCGG
MspI 2526 CCGG C^CGG
2892 CCGG
PpuMI 2722 AGGTCCC [GA]G^G[AT]CC[CT]
2818 AGGTCCC
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BglII BmyI BpuAI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmnI