Restriction map for
pF1KB3095
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
Acc65I AccII AccIII AclI AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaJI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 935 GACGTC GACGT^C
AccI 201 GTATAC GT^[AC][GT]AC
AcyI 935 GACGTC G[GA]^CG[CT]C
AfaI 52 GTAC GT^AC
AflII 357 CTTAAG C^TTAAG
AhdI 935 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 619 GATCC GGATC(4/5)
AspEI 935 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 734 GAGCTC G[AT]GC[AT]^C
AvaI 122 CTCGGG C^[CT]CG[GA]G
BanII 734 GAGCTC G[GA]GC[CT]^C
BbsI 444 GAAGAC GAAGAC(2/6)
BcnI 38 CCCGG CC^[GC]GG
BfaI 380 CTAG C^TAG
BfrI 357 CTTAAG C^TTAAG
BmyI 734 GAGCTC G[AGT]GC[ACT]^C
BpmI 46 CTGGAG CTGGAG(16/14)
BpuAI 444 GAAGAC GAAGAC(2/6)
BsaHI 935 GACGTC G[GA]^CG[CT]C
BsaI 567 GAGACC GGTCTC(1/5)
BsgI 79 GTGCAG GTGCAG(16/14)
BsiHKAI 734 GAGCTC G[AT]GC[AT]^C
BsmFI 580 GTCCC GGGAC(10/14)
BsmI 412 GAATGC GAATGC(1/-1)
BsoBI 122 CTCGGG C^[CT]CG[GA]G
Bsp1286I 734 GAGCTC G[AGT]GC[ACT]^C
BspHI 711 TCATGA T^CATGA
BspMI 533 ACCTGC ACCTGC(4/8)
BsrI 912 CCAGT ACTGG(1/-1)
Bst1107I 201 GTATAC GTA^TAC
BstEII 786 GGTAACC G^GT[ACGT]ACC
BstNI 67 CCAGG CC^[AT]GG
BstPI 786 GGTAACC G^GT[ACGT]ACC
BstXI 912 CCAGTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 201 GTATAC GTA^TAC
Eam1105I 935 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 583 CCTGATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 786 GGTAACC G^GT[ACGT]ACC
EcoRI 927 GAATTC G^AATTC
EcoRII 67 CCAGG ^CC[AT]GG
HapII 39 CCGG C^CGG
Hin1I 935 GACGTC G[GA]^CG[CT]C
HpaII 39 CCGG C^CGG
MaeI 380 CTAG C^TAG
MslI 370 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 169 CCGCTG C[AC]G^C[GT]G
MspI 39 CCGG C^CGG
MvaI 67 CCAGG CC^[AT]GG
MwoI 383 GCATCAATCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 38 CCCGG CC^[GC]GG
NheI 379 GCTAGC G^CTAGC
RcaI 711 TCATGA T^CATGA
RsaI 52 GTAC GT^AC
SacI 734 GAGCTC GAGCT^C
SfaNI 383 GCATC GCATC(5/9)
SfcI 476 CTATAG C^T[GA][CT]AG
SmlI 357 CTTAAG C^T[CT][GA]AG
SspI 430 AATATT AAT^ATT
TspRI 453 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 444 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
498 GAAGATCTTC
BbvI 481 GCAGC GCAGC(8/12)
42 GCTGC
BglII 128 AGATCT A^GATCT
500 AGATCT
BsiYI 39 CCGGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCTGATACAGG
BslI 39 CCGGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCTGATACAGG
BsmAI 567 GAGAC GTCTC(1/5)
120 GTCTC
BssKI 38 CCCGG ^CC[ACGT]GG
67 CCAGG
BstYI 128 AGATCT [GA]^GATC[CT]
500 AGATCT
Cac8I 140 GCCAGC GC[ACGT]^[ACGT]GC
379 GCTAGC
Fnu4HI 42 GCTGC GC^[ACGT]GC
481 GCAGC
HphI 35 TCACC GGTGA(8/7)
526 TCACC
ItaI 42 GCTGC GC^[ACGT]GC
481 GCAGC
MaeII 20 ACGT A^CGT
936 ACGT
MflI 128 AGATCT [GA]^GATC[CT]
500 AGATCT
ScrFI 38 CCCGG CC^[ACGT]GG
67 CCAGG
TseI 42 GCTGC G^C[AT]GC
481 GCAGC
Tsp45I 525 GTCAC ^GT[GC]AC
943 GTCAC
XhoII 128 AGATCT [GA]^GATC[CT]
500 AGATCT
XmnI 444 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
498 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AcsI AluI ApoI BstF5I
DdeI DpnII FokI HinfI MaeIII
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI TaqI TfiI Tru9I
Tsp509I TthHB8I