Restriction map for
pF1KB3372
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbsI BbvCI
BcgI BclI BcnI BfaI BglII
BlnI BlpI Bpu1102I BpuAI BsaI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HpaI KpnI KspI MaeI MfeI
MluI MroI MunI NciI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AflII 1056 CTTAAG C^TTAAG
AflIII 446 ACACGT A^C[GA][CT]GT
AhdI 181 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 460 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 181 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 709 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 516 CTCGGG C^[CT]CG[GA]G
BalI 161 TGGCCA TGG^CCA
BbeI 232 GGCGCC GGCGC^C
BbrPI 447 CACGTG CAC^GTG
BfrI 1056 CTTAAG C^TTAAG
BsaAI 447 CACGTG [CT]AC^GT[GA]
BsaBI 141 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 437 ACCGGA [AT]^CCGG[AT]
BseRI 956 CTCCTC GAGGAG(10/8)
BsiEI 120 CGGCCG CG[GA][CT]^CG
BsmI 793 GCATTC GAATGC(1/-1)
BsoBI 516 CTCGGG C^[CT]CG[GA]G
BspHI 74 TCATGA T^CATGA
BsrFI 346 GCCGGC [GA]^CCGG[CT]
BssSI 205 CACGAG CACGAG(-5/-1)
BstAPI 687 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 346 GCCGGC [GA]^CCGG[CT]
EagI 120 CGGCCG C^GGCCG
Eam1105I 181 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 394 GAAGAG CTCTTC(1/4)
EclXI 120 CGGCCG C^GGCCG
Eco52I 120 CGGCCG C^GGCCG
EcoNI 852 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1078 GAATTC G^AATTC
EcoRV 1157 GATATC GAT^ATC
HincII 189 GTCGAC GT[CT]^[GA]AC
HindII 189 GTCGAC GT[CT]^[GA]AC
HindIII 1053 AAGCTT A^AGCTT
KasI 232 GGCGCC G^GCGCC
Ksp632I 394 GAAGAG CTCTTC(1/4)
MamI 141 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 394 GAAGA GAAGA(8/7)
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
MseI 1057 TTAA T^TAA
MslI 1230 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 346 GCCGGC GCC^GGC
NarI 232 GGCGCC GG^CGCC
NcoI 1205 CCATGG C^CATGG
NgoMIV 346 GCCGGC G^CCGGC
PflFI 709 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 447 CACGTG CAC^GTG
PmlI 447 CACGTG CAC^GTG
PvuII 1007 CAGCTG CAG^CTG
RcaI 74 TCATGA T^CATGA
SalI 189 GTCGAC G^TCGAC
SbfI 684 CCTGCAGG CCTGCA^GG
SfiI 162 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 232 GGCGCC GGC^GCC
Sse8387I 684 CCTGCAGG CCTGCA^GG
StuI 599 AGGCCT AGG^CCT
Tru9I 1057 TTAA T^TAA
Tth111I 709 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 263 CCAGGGAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 189 GTCGAC GT^[AC][GT]AC
676 GTCTAC
AcsI 47 GAATTT [GA]^AATT[CT]
1078 GAATTC
AcyI 232 GGCGCC G[GA]^CG[CT]C
984 GGCGTC
ApoI 47 GAATTT [GA]^AATT[CT]
1078 GAATTC
BanI 232 GGCGCC G^G[CT][GA]CC
1144 GGTGCC
BciVI 866 GGATAC GTATCC(6/5)
59 GTATCC
BglI 163 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
551 GCCTCATTGGC
BsaHI 232 GGCGCC G[GA]^CG[CT]C
984 GGCGTC
BsmBI 714 CGTCTC CGTCTC(1/5)
541 GAGACG
BsmFI 1307 GGGAC GGGAC(10/14)
657 GTCCC
BsrBI 521 GAGCGG CCGCTC(-3/-3)
652 GAGCGG
BsrI 990 ACTGG ACTGG(1/-1)
1234 CCAGT
BstYI 500 AGATCC [GA]^GATC[CT]
1019 AGATCC
EaeI 120 CGGCCG [CT]^GGCC[GA]
161 TGGCCA
HapII 347 CCGG C^CGG
438 CCGG
Hin1I 232 GGCGCC G[GA]^CG[CT]C
984 GGCGTC
HpaII 347 CCGG C^CGG
438 CCGG
HphI 254 TCACC GGTGA(8/7)
260 TCACC
MflI 500 AGATCC [GA]^GATC[CT]
1019 AGATCC
MspA1I 401 CAGCGG C[AC]G^C[GT]G
1007 CAGCTG
MspI 347 CCGG C^CGG
438 CCGG
PpuMI 155 AGGACCT [GA]G^G[AT]CC[CT]
269 AGGACCT
SacI 352 GAGCTC GAGCT^C
1135 GAGCTC
SmlI 853 CTCAAG C^T[CT][GA]AG
1056 CTTAAG
TfiI 608 GATTC G^A[AT]TC
942 GAATC
XhoII 500 AGATCC [GA]^GATC[CT]
1019 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinP1I
HinfI ItaI MaeII MaeIII MboI
MnlI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I