##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        153  GGTACC               G^GTACC
AccII          75  CGCG                 CG^CG
AciI           74  CCGC                 CCGC(-3/-1)
AfaI          154  GTAC                 GT^AC
AlwI           71  GATCC                GGATC(4/5)
Asp718I       153  GGTACC               G^GTACC
BanI          153  GGTACC               G^G[CT][GA]CC
BanII         112  GAGCCC               G[GA]GC[CT]^C
BmyI          112  GAGCCC               G[AGT]GC[ACT]^C
BpmI          257  CTGGAG               CTGGAG(16/14)
BseRI          98  CTCCTC               GAGGAG(10/8)
Bsp1286I      112  GAGCCC               G[AGT]GC[ACT]^C
BspMI          93  ACCTGC               ACCTGC(4/8)
BssKI         234  CCAGG                ^CC[ACGT]GG
BstF5I         24  GGATG                GGATG(2/0)
BstNI         234  CCAGG                CC^[AT]GG
BstUI          75  CGCG                 CG^CG
BstYI          70  AGATCC               [GA]^GATC[CT]
Cac8I         184  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        129  GGCCC                G^G[ACGT]CC
DdeI           29  CTAAG                C^T[ACGT]AG
DpnII          71  GATC                 ^GATC
DraII         128  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      128  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        234  CCAGG                ^CC[AT]GG
FokI           24  GGATG                GGATG(9/13)
HaeIII        129  GGCC                 GG^CC
HphI           48  GGTGA                GGTGA(8/7)
KpnI          153  GGTACC               GGTAC^C
MboI           71  GATC                 ^GATC
MboII          68  GAAGA                GAAGA(8/7)
MflI           70  AGATCC               [GA]^GATC[CT]
MvaI          234  CCAGG                CC^[AT]GG
MvnI           75  CGCG                 CG^CG
NdeII          71  GATC                 ^GATC
PstI          206  CTGCAG               CTGCA^G
RsaI          154  GTAC                 GT^AC
Sau3AI         71  GATC                 ^GATC
Sau96I        129  GGCCC                G^G[ACGT]CC
ScrFI         234  CCAGG                CC^[ACGT]GG
SfcI          206  CTGCAG               C^T[GA][CT]AG
XhoII          70  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

AluI          177  AGCT                 AG^CT
              246  AGCT                
NlaIV         129  GGCCCC               GG[ACGT]^[ACGT]CC
              153  GGTACC              


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MnlI        MwoI        SfaNI