Restriction map for
pF1KB3391
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbsI BcgI BciVI
BfrI BlnI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1286I BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraII DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 307 GGTACC G^GTACC
AcyI 464 GACGCC G[GA]^CG[CT]C
AhdI 530 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 307 GGTACC G^GTACC
AspEI 530 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 307 GGTACC G^G[CT][GA]CC
BbrPI 406 CACGTG CAC^GTG
BbvCI 793 CCTCAGC CCTCAGC(-5/-2)
BglI 467 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 692 AGATCT A^GATCT
BpmI 799 CTCCAG CTGGAG(16/14)
BsaAI 406 CACGTG [CT]AC^GT[GA]
BsaHI 464 GACGCC G[GA]^CG[CT]C
BsmFI 439 GGGAC GGGAC(10/14)
Bsp1407I 203 TGTACA T^GTACA
BspDI 601 ATCGAT AT^CGAT
BsrGI 203 TGTACA T^GTACA
BstEII 88 GGTAACC G^GT[ACGT]ACC
BstF5I 453 CATCC GGATG(2/0)
BstPI 88 GGTAACC G^GT[ACGT]ACC
BstYI 692 AGATCT [GA]^GATC[CT]
CfoI 735 GCGC GCG^C
ClaI 601 ATCGAT AT^CGAT
DsaI 381 CCACGG C^C[GA][CT]GG
Eam1105I 530 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 88 GGTAACC G^GT[ACGT]ACC
EcoRV 604 GATATC GAT^ATC
EcoT14I 233 CCAAGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
FokI 453 CATCC GGATG(9/13)
HaeIII 198 GGCC GG^CC
HgaI 464 GACGC GACGC(5/10)
HhaI 735 GCGC GCG^C
Hin1I 464 GACGCC G[GA]^CG[CT]C
HinP1I 735 GCGC G^CGC
HinfI 760 GACTC G^A[ACGT]TC
KpnI 307 GGTACC GGTAC^C
MaeII 407 ACGT A^CGT
MflI 692 AGATCT [GA]^GATC[CT]
MseI 320 TTAA T^TAA
MspA1I 499 CAGCTG C[AC]G^C[GT]G
PflMI 283 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 760 GACTC GAGTC(4/5)
PmaCI 406 CACGTG CAC^GTG
PmlI 406 CACGTG CAC^GTG
PvuII 499 CAGCTG CAG^CTG
ScaI 215 AGTACT AGT^ACT
SspBI 203 TGTACA T^GTACA
StyI 233 CCAAGG C^C[AT][AT]GG
Tru9I 320 TTAA T^TAA
TspRI 459 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 283 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 692 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 311 CCCGGG C^[CT]CG[GA]G
436 CCCGGG
BclI 449 TGATCA T^GATCA
744 TGATCA
BfaI 338 CTAG C^TAG
506 CTAG
BsoBI 311 CCCGGG C^[CT]CG[GA]G
436 CCCGGG
BspMI 809 ACCTGC ACCTGC(4/8)
164 GCAGGT
BstNI 681 CCAGG CC^[AT]GG
801 CCAGG
DdeI 219 CTCAG C^T[ACGT]AG
794 CTCAG
EcoRII 681 CCAGG ^CC[AT]GG
801 CCAGG
FbaI 449 TGATCA T^GATCA
744 TGATCA
HapII 312 CCGG C^CGG
437 CCGG
HpaII 312 CCGG C^CGG
437 CCGG
MaeI 338 CTAG C^TAG
506 CTAG
MspI 312 CCGG C^CGG
437 CCGG
MvaI 681 CCAGG CC^[AT]GG
801 CCAGG
NlaIII 183 CATG CATG^
201 CATG
NlaIV 307 GGTACC GG[ACGT]^[ACGT]CC
439 GGGACC
SfaNI 64 GATGC GCATC(5/9)
640 GATGC
SmaI 311 CCCGGG CCC^GGG
436 CCCGGG
Tsp45I 293 GTGAC ^GT[GC]AC
523 GTGAC
XmaCI 311 CCCGGG C^CCGGG
436 CCCGGG
XmaI 311 CCCGGG C^CCGGG
436 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BcnI BsaJI BsiYI
BslI BsrDI BsrI BssKI Cac8I
Cfr13I DpnII Fnu4HI HphI ItaI
MaeIII MboI MboII MnlI MwoI
NciI NdeII RsaI Sau3AI Sau96I
ScrFI TaqI TseI Tsp509I TthHB8I