################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI KspI MamI MfeI MflI MluI MroI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 521 ACGTGT A^C[GA][CT]GT AlwI 471 GATCC GGATC(4/5) AvaI 77 CTCGGG C^[CT]CG[GA]G AvaII 576 GGACC G^G[AT]CC AvrII 327 CCTAGG C^CTAGG BalI 272 TGGCCA TGG^CCA BlnI 327 CCTAGG C^CTAGG BpmI 104 CTGGAG CTGGAG(16/14) BsaI 482 GAGACC GGTCTC(1/5) BsiYI 17 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 17 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 77 CTCGGG C^[CT]CG[GA]G BsrDI 294 CATTGC GCAATG(2/0) BsrFI 188 GCCGGC [GA]^CCGG[CT] BstF5I 459 GGATG GGATG(2/0) CfoI 516 GCGC GCG^C Cfr10I 188 GCCGGC [GA]^CCGG[CT] EaeI 272 TGGCCA [CT]^GGCC[GA] EcoT14I 327 CCTAGG C^C[AT][AT]GG FokI 459 GGATG GGATG(9/13) HapII 189 CCGG C^CGG HhaI 516 GCGC GCG^C HinP1I 516 GCGC G^CGC HpaI 490 GTTAAC GTT^AAC HpaII 189 CCGG C^CGG MluNI 272 TGGCCA TGG^CCA MscI 272 TGGCCA TGG^CCA MseI 491 TTAA T^TAA MspA1I 477 CAGCGG C[AC]G^C[GT]G MspI 189 CCGG C^CGG NaeI 188 GCCGGC GCC^GGC NgoMIV 188 GCCGGC G^CCGGC PleI 307 GAGTC GAGTC(4/5) PpuMI 575 GGGACCC [GA]G^G[AT]CC[CT] SfcI 212 CTGTAG C^T[GA][CT]AG StyI 327 CCTAGG C^C[AT][AT]GG TfiI 195 GATTC G^A[AT]TC Tru9I 491 TTAA T^TAA XcmI 446 CCATGAAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BbrPI 520 CACGTG CAC^GTG 619 CACGTG BfaI 278 CTAG C^TAG 328 CTAG BsaAI 520 CACGTG [CT]AC^GT[GA] 619 CACGTG BsmAI 482 GAGAC GTCTC(1/5) 309 GTCTC BsmFI 575 GGGAC GGGAC(10/14) 718 GTCCC BsrI 583 ACTGG ACTGG(1/-1) 715 CCAGT DpnII 471 GATC ^GATC 561 GATC DraII 349 AGGGCCT [GA]G^G[ACGT]CC[CT] 575 GGGACCC EarI 21 GAAGAG CTCTTC(1/4) 222 GAAGAG EcoO109I 349 AGGGCCT [GA]G^G[ACGT]CC[CT] 575 GGGACCC HincII 490 GTTAAC GT[CT]^[GA]AC 502 GTTGAC HindII 490 GTTAAC GT[CT]^[GA]AC 502 GTTGAC HinfI 195 GATTC G^A[ACGT]TC 307 GAGTC HphI 178 GGTGA GGTGA(8/7) 555 GGTGA Ksp632I 21 GAAGAG CTCTTC(1/4) 222 GAAGAG MaeI 278 CTAG C^TAG 328 CTAG MaeIII 121 GTTAC ^GT[ACGT]AC 317 GTTAC MboI 471 GATC ^GATC 561 GATC MslI 507 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 549 CATCAAGGTG NdeII 471 GATC ^GATC 561 GATC PmaCI 520 CACGTG CAC^GTG 619 CACGTG PmlI 520 CACGTG CAC^GTG 619 CACGTG Sau3AI 471 GATC ^GATC 561 GATC SfaNI 44 GCATC GCATC(5/9) 548 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BbvI BsaJI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MaeII MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI TspRI