Restriction map for pF1KB3421
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       BamHI       BanI        
BanII       BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlpI        BmyI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MunI        MvnI        NarI        NciI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflIII        521  ACGTGT               A^C[GA][CT]GT
AlwI          471  GATCC                GGATC(4/5)
AvaI           77  CTCGGG               C^[CT]CG[GA]G
AvaII         576  GGACC                G^G[AT]CC
AvrII         327  CCTAGG               C^CTAGG
BalI          272  TGGCCA               TGG^CCA
BlnI          327  CCTAGG               C^CTAGG
BpmI          104  CTGGAG               CTGGAG(16/14)
BsaI          482  GAGACC               GGTCTC(1/5)
BsiYI          17  CCAAGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           17  CCAAGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI          77  CTCGGG               C^[CT]CG[GA]G
BsrDI         294  CATTGC               GCAATG(2/0)
BsrFI         188  GCCGGC               [GA]^CCGG[CT]
BstF5I        459  GGATG                GGATG(2/0)
CfoI          516  GCGC                 GCG^C
Cfr10I        188  GCCGGC               [GA]^CCGG[CT]
EaeI          272  TGGCCA               [CT]^GGCC[GA]
EcoT14I       327  CCTAGG               C^C[AT][AT]GG
FokI          459  GGATG                GGATG(9/13)
HapII         189  CCGG                 C^CGG
HhaI          516  GCGC                 GCG^C
HinP1I        516  GCGC                 G^CGC
HpaI          490  GTTAAC               GTT^AAC
HpaII         189  CCGG                 C^CGG
MluNI         272  TGGCCA               TGG^CCA
MscI          272  TGGCCA               TGG^CCA
MseI          491  TTAA                 T^TAA
MspA1I        477  CAGCGG               C[AC]G^C[GT]G
MspI          189  CCGG                 C^CGG
NaeI          188  GCCGGC               GCC^GGC
NgoMIV        188  GCCGGC               G^CCGGC
PleI          307  GAGTC                GAGTC(4/5)
PpuMI         575  GGGACCC              [GA]G^G[AT]CC[CT]
SfcI          212  CTGTAG               C^T[GA][CT]AG
StyI          327  CCTAGG               C^C[AT][AT]GG
TfiI          195  GATTC                G^A[AT]TC
Tru9I         491  TTAA                 T^TAA
XcmI          446  CCATGAAGTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BbrPI         520  CACGTG               CAC^GTG
              619  CACGTG              
BfaI          278  CTAG                 C^TAG
              328  CTAG                
BsaAI         520  CACGTG               [CT]AC^GT[GA]
              619  CACGTG              
BsmAI         482  GAGAC                GTCTC(1/5)
              309  GTCTC               
BsmFI         575  GGGAC                GGGAC(10/14)
              718  GTCCC               
BsrI          583  ACTGG                ACTGG(1/-1)
              715  CCAGT               
DpnII         471  GATC                 ^GATC
              561  GATC                
DraII         349  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              575  GGGACCC             
EarI           21  GAAGAG               CTCTTC(1/4)
              222  GAAGAG              
EcoO109I      349  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              575  GGGACCC             
HincII        490  GTTAAC               GT[CT]^[GA]AC
              502  GTTGAC              
HindII        490  GTTAAC               GT[CT]^[GA]AC
              502  GTTGAC              
HinfI         195  GATTC                G^A[ACGT]TC
              307  GAGTC               
HphI          178  GGTGA                GGTGA(8/7)
              555  GGTGA               
Ksp632I        21  GAAGAG               CTCTTC(1/4)
              222  GAAGAG              
MaeI          278  CTAG                 C^TAG
              328  CTAG                
MaeIII        121  GTTAC                ^GT[ACGT]AC
              317  GTTAC               
MboI          471  GATC                 ^GATC
              561  GATC                
MslI          507  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              549  CATCAAGGTG          
NdeII         471  GATC                 ^GATC
              561  GATC                
PmaCI         520  CACGTG               CAC^GTG
              619  CACGTG              
PmlI          520  CACGTG               CAC^GTG
              619  CACGTG              
Sau3AI        471  GATC                 ^GATC
              561  GATC                
SfaNI          44  GCATC                GCATC(5/9)
              548  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BssKI       
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
Fnu4HI      HaeIII      ItaI        MaeII       MboII       
MnlI        MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       TseI        TspRI       
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