Restriction map for
pF1KB3433
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AvaII AvrII BamHI
BbrPI BcgI BclI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FbaI FseI HgaI
HincII HindII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MslI
MunI MvnI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 770 TCCGGA T^CCGGA
AcsI 224 GAATTC [GA]^AATT[CT]
AcyI 323 GGCGCC G[GA]^CG[CT]C
AflII 511 CTTAAG C^TTAAG
AflIII 281 ACATGT A^C[GA][CT]GT
ApaI 615 GGGCCC GGGCC^C
ApoI 224 GAATTC [GA]^AATT[CT]
AseI 853 ATTAAT AT^TAAT
AsnI 853 ATTAAT AT^TAAT
AviII 131 TGCGCA TGC^GCA
BalI 261 TGGCCA TGG^CCA
BanI 323 GGCGCC G^G[CT][GA]CC
BbeI 323 GGCGCC GGCGC^C
BbsI 486 GAAGAC GAAGAC(2/6)
BbvCI 389 CCTCAGC CCTCAGC(-5/-2)
BciVI 602 GTATCC GTATCC(6/5)
BcnI 1047 CCGGG CC^[GC]GG
BfaI 185 CTAG C^TAG
BfrI 511 CTTAAG C^TTAAG
BglII 861 AGATCT A^GATCT
BpuAI 486 GAAGAC GAAGAC(2/6)
BsaHI 323 GGCGCC G[GA]^CG[CT]C
BsaWI 770 TCCGGA [AT]^CCGG[AT]
BseAI 770 TCCGGA T^CCGGA
BseRI 1261 GAGGAG GAGGAG(10/8)
BspEI 770 TCCGGA T^CCGGA
BspLU11I 281 ACATGT A^CATGT
BspMI 172 GCAGGT ACCTGC(4/8)
BsrBI 255 CCGCTC CCGCTC(-3/-3)
BstAPI 1060 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 669 CATCC GGATG(2/0)
DraII 614 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 261 TGGCCA [CT]^GGCC[GA]
EcoNI 1126 CCTGTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 614 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 224 GAATTC G^AATTC
EcoT14I 491 CCAAGG C^C[AT][AT]GG
FokI 669 CATCC GGATG(9/13)
FspI 131 TGCGCA TGC^GCA
HaeII 323 GGCGCC [GA]GCGC^[CT]
Hin1I 323 GGCGCC G[GA]^CG[CT]C
HindIII 715 AAGCTT A^AGCTT
HphI 252 TCACC GGTGA(8/7)
KasI 323 GGCGCC G^GCGCC
MaeI 185 CTAG C^TAG
MaeII 741 ACGT A^CGT
MluNI 261 TGGCCA TGG^CCA
MroI 770 TCCGGA T^CCGGA
MscI 261 TGGCCA TGG^CCA
NarI 323 GGCGCC GG^CGCC
NciI 1047 CCGGG CC^[GC]GG
NdeI 989 CATATG CA^TATG
PflMI 314 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 853 ATTAAT AT^TAAT
PvuII 1061 CAGCTG CAG^CTG
SfoI 323 GGCGCC GGC^GCC
SpeI 184 ACTAGT A^CTAGT
StuI 656 AGGCCT AGG^CCT
StyI 491 CCAAGG C^C[AT][AT]GG
Tsp45I 64 GTCAC ^GT[GC]AC
Van91I 314 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 891 GATCC GGATC(4/5)
924 GATCC
AlwNI 1061 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1220 CAGCCCCTG
AspHI 8 GTGCTC G[AT]GC[AT]^C
908 GAGCAC
BanII 615 GGGCCC G[GA]GC[CT]^C
899 GAGCCC
BsiHKAI 8 GTGCTC G[AT]GC[AT]^C
908 GAGCAC
BsmFI 247 GGGAC GGGAC(10/14)
738 GGGAC
BsrI 205 ACTGG ACTGG(1/-1)
405 ACTGG
Cfr13I 615 GGGCC G^G[ACGT]CC
616 GGCCC
EcoRV 21 GATATC GAT^ATC
535 GATATC
MaeIII 64 GTCAC ^GT[ACGT]AC
824 GTAAC
MspA1I 1061 CAGCTG C[AC]G^C[GT]G
1073 CAGCGG
NspI 281 ACATGT [GA]CATG^[CT]
732 GCATGT
PstI 1067 CTGCAG CTGCA^G
1238 CTGCAG
Sau96I 615 GGGCC G^G[ACGT]CC
616 GGCCC
SfaNI 333 GCATC GCATC(5/9)
623 GCATC
XcmI 264 CCAGGGTGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
310 CCAACCAAAACCTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI BstYI Cac8I CfoI DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII