Restriction map for
pF1KB3511
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BbrPI BbsI BciVI
BclI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MluI MroI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 886 GACGTC GACGT^C
AocI 736 CCTCAGG CC^T[ACGT]AGG
Asp700I 861 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1135 TGGCCA TGG^CCA
BamHI 379 GGATCC G^GATCC
BbeI 519 GGCGCC GGCGC^C
BbvCI 349 CCTCAGC CCTCAGC(-5/-2)
BcgI 1059 CGATCTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 872 CTAG C^TAG
BlpI 1300 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1300 GCTCAGC GC^T[ACGT]AGC
BseRI 787 GAGGAG GAGGAG(10/8)
BspMI 816 GCAGGT ACCTGC(4/8)
BsrDI 1065 CATTGC GCAATG(2/0)
BstAPI 1347 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 736 CCTCAGG CC^T[ACGT]AGG
CelII 1300 GCTCAGC GC^T[ACGT]AGC
EaeI 1135 TGGCCA [CT]^GGCC[GA]
Eco81I 736 CCTCAGG CC^T[ACGT]AGG
EcoNI 810 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 274 GAATTC G^AATTC
FauI 14 CCCGC CCCGC(4/6)
HaeII 519 GGCGCC [GA]GCGC^[CT]
HphI 1222 GGTGA GGTGA(8/7)
KasI 519 GGCGCC G^GCGCC
MaeI 872 CTAG C^TAG
MaeII 887 ACGT A^CGT
MaeIII 341 GTGAC ^GT[ACGT]AC
MfeI 537 CAATTG C^AATTG
MluNI 1135 TGGCCA TGG^CCA
MscI 1135 TGGCCA TGG^CCA
MslI 720 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 537 CAATTG C^AATTG
NarI 519 GGCGCC GG^CGCC
NcoI 515 CCATGG C^CATGG
PshAI 763 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 790 GAGCTC GAGCT^C
SfaNI 1322 GCATC GCATC(5/9)
SfoI 519 GGCGCC GGC^GCC
SphI 1370 GCATGC GCATG^C
StuI 173 AGGCCT AGG^CCT
TaqI 364 TCGA T^CGA
TfiI 111 GATTC G^A[AT]TC
Tsp45I 341 GTGAC ^GT[GC]AC
TthHB8I 364 TCGA T^CGA
XcmI 48 CCAGGTGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 861 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 274 GAATTC [GA]^AATT[CT]
823 AAATTC
AcyI 519 GGCGCC G[GA]^CG[CT]C
886 GACGTC
ApoI 274 GAATTC [GA]^AATT[CT]
823 AAATTC
AspHI 254 GTGCTC G[AT]GC[AT]^C
790 GAGCTC
BcnI 131 CCCGG CC^[GC]GG
561 CCCGG
BglII 1109 AGATCT A^GATCT
1265 AGATCT
BsaHI 519 GGCGCC G[GA]^CG[CT]C
886 GACGTC
BsiHKAI 254 GTGCTC G[AT]GC[AT]^C
790 GAGCTC
BsmAI 187 GAGAC GTCTC(1/5)
1014 GAGAC
BstXI 226 CCAGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
640 CCAGAACTGTGG
CfoI 249 GCGC GCG^C
520 GCGC
DsaI 515 CCATGG C^C[GA][CT]GG
696 CCGTGG
EarI 751 CTCTTC CTCTTC(1/4)
151 GAAGAG
EcoT14I 299 CCAAGG C^C[AT][AT]GG
515 CCATGG
HhaI 249 GCGC GCG^C
520 GCGC
Hin1I 519 GGCGCC G[GA]^CG[CT]C
886 GACGTC
HinP1I 249 GCGC G^CGC
520 GCGC
Ksp632I 751 CTCTTC CTCTTC(1/4)
151 GAAGAG
MseI 170 TTAA T^TAA
371 TTAA
NciI 131 CCCGG CC^[GC]GG
561 CCCGG
NspI 123 ACATGC [GA]CATG^[CT]
1370 GCATGC
PstI 555 CTGCAG CTGCA^G
1261 CTGCAG
StyI 299 CCAAGG C^C[AT][AT]GG
515 CCATGG
Tru9I 170 TTAA T^TAA
371 TTAA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp509I TspRI XhoII