Restriction map for
pF1KB3544
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DdeI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TseI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 55 CGCG CG^CG
AciI 56 GCGG CCGC(-3/-1)
AcsI 106 AAATTT [GA]^AATT[CT]
AluI 308 AGCT AG^CT
Alw44I 73 GTGCAC G^TGCAC
AlwI 168 GATCC GGATC(4/5)
ApaLI 73 GTGCAC G^TGCAC
ApoI 106 AAATTT [GA]^AATT[CT]
AspHI 73 GTGCAC G[AT]GC[AT]^C
AvaI 119 CCCGGG C^[CT]CG[GA]G
BbsI 295 GTCTTC GAAGAC(2/6)
BfaI 151 CTAG C^TAG
BpmI 233 CTCCAG CTGGAG(16/14)
BpuAI 295 GTCTTC GAAGAC(2/6)
BsgI 7 GTGCAG GTGCAG(16/14)
BsiHKAI 73 GTGCAC G[AT]GC[AT]^C
BsmAI 32 GAGAC GTCTC(1/5)
BsmBI 32 GAGACG CGTCTC(1/5)
BsmFI 122 GGGAC GGGAC(10/14)
BsoBI 119 CCCGGG C^[CT]CG[GA]G
BstUI 55 CGCG CG^CG
Cac8I 155 GCAAGC GC[ACGT]^[ACGT]GC
DpnII 168 GATC ^GATC
EaeI 57 CGGCCA [CT]^GGCC[GA]
Fnu4HI 56 GCGGC GC^[ACGT]GC
HaeIII 58 GGCC GG^CC
HinfI 112 GATTC G^A[ACGT]TC
HphI 165 GGTGA GGTGA(8/7)
ItaI 56 GCGGC GC^[ACGT]GC
MaeI 151 CTAG C^TAG
MboI 168 GATC ^GATC
MseI 140 TTAA T^TAA
MslI 81 CACCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 55 CGCG CG^CG
NdeII 168 GATC ^GATC
NlaIII 283 CATG CATG^
NspI 282 ACATGC [GA]CATG^[CT]
Sau3AI 168 GATC ^GATC
SmaI 119 CCCGGG CCC^GGG
TaqI 299 TCGA T^CGA
TfiI 112 GATTC G^A[AT]TC
Tru9I 140 TTAA T^TAA
Tsp509I 107 AATT ^AATT
TspRI 255 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 299 TCGA T^CGA
XmaCI 119 CCCGGG C^CCGGG
XmaI 119 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 250 GGTGCC G^G[CT][GA]CC
260 GGCACC
BmyI 73 GTGCAC G[AGT]GC[ACT]^C
259 GGGCAC
Bsp1286I 73 GTGCAC G[AGT]GC[ACT]^C
259 GGGCAC
BspMI 64 ACCTGC ACCTGC(4/8)
9 GCAGGT
BsrI 256 ACTGG ACTGG(1/-1)
318 ACTGG
BstF5I 273 CATCC GGATG(2/0)
86 GGATG
BstNI 28 CCAGG CC^[AT]GG
265 CCAGG
CfoI 39 GCGC GCG^C
54 GCGC
EcoRII 28 CCAGG ^CC[AT]GG
265 CCAGG
FokI 273 CATCC GGATG(9/13)
86 GGATG
HapII 83 CCGG C^CGG
120 CCGG
HhaI 39 GCGC GCG^C
54 GCGC
HinP1I 39 GCGC G^CGC
54 GCGC
HpaII 83 CCGG C^CGG
120 CCGG
MspI 83 CCGG C^CGG
120 CCGG
MvaI 28 CCAGG CC^[AT]GG
265 CCAGG
NlaIV 250 GGTGCC GG[ACGT]^[ACGT]CC
260 GGCACC
SfaNI 87 GATGC GCATC(5/9)
269 GCATC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BcnI BsaJI BsiYI BslI BssKI
MboII MnlI MwoI NciI ScrFI