Restriction map for
pF1KB3561
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AcsI AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 514 AACGTT AA^CGTT
AfaI 21 GTAC GT^AC
AflIII 437 ACGTGT A^C[GA][CT]GT
AgeI 9 ACCGGT A^CCGGT
AlwI 69 GATCC GGATC(4/5)
AlwNI 196 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 55 CCCGGG C^[CT]CG[GA]G
BalI 500 TGGCCA TGG^CCA
BanI 6 GGCACC G^G[CT][GA]CC
BbvCI 105 GCTGAGG CCTCAGC(-5/-2)
BclI 362 TGATCA T^GATCA
BglI 347 GCCACGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 575 CTCCAG CTGGAG(16/14)
BsaAI 436 TACGTG [CT]AC^GT[GA]
BsaWI 9 ACCGGT [AT]^CCGG[AT]
BseRI 421 CTCCTC GAGGAG(10/8)
BsgI 139 GTGCAG GTGCAG(16/14)
BsmAI 382 GTCTC GTCTC(1/5)
BsoBI 55 CCCGGG C^[CT]CG[GA]G
BsrFI 9 ACCGGT [GA]^CCGG[CT]
BstEII 12 GGTAACC G^GT[ACGT]ACC
BstPI 12 GGTAACC G^GT[ACGT]ACC
BstYI 68 AGATCC [GA]^GATC[CT]
Cfr10I 9 ACCGGT [GA]^CCGG[CT]
DraIII 433 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 500 TGGCCA [CT]^GGCC[GA]
EcoNI 75 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 12 GGTAACC G^GT[ACGT]ACC
EcoT14I 596 CCATGG C^C[AT][AT]GG
FauI 410 CCCGC CCCGC(4/6)
FbaI 362 TGATCA T^GATCA
HincII 121 GTCGAC GT[CT]^[GA]AC
HindII 121 GTCGAC GT[CT]^[GA]AC
HinfI 495 GATTC G^A[ACGT]TC
MflI 68 AGATCC [GA]^GATC[CT]
MluNI 500 TGGCCA TGG^CCA
MscI 500 TGGCCA TGG^CCA
MspA1I 313 CAGCGG C[AC]G^C[GT]G
NcoI 596 CCATGG C^CATGG
PinAI 9 ACCGGT A^CCGGT
Psp1406I 514 AACGTT AA^CGTT
PstI 202 CTGCAG CTGCA^G
RsaI 21 GTAC GT^AC
SacI 234 GAGCTC GAGCT^C
SalI 121 GTCGAC G^TCGAC
SfcI 202 CTGCAG C^T[GA][CT]AG
SmaI 55 CCCGGG CCC^GGG
StyI 596 CCATGG C^C[AT][AT]GG
TaqI 122 TCGA T^CGA
TfiI 495 GATTC G^A[AT]TC
TspRI 540 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 122 TCGA T^CGA
XhoII 68 AGATCC [GA]^GATC[CT]
XmaCI 55 CCCGGG C^CCGGG
XmaI 55 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 121 GTCGAC GT^[AC][GT]AC
448 GTAGAC
AccII 64 CGCG CG^CG
91 CGCG
AspHI 234 GAGCTC G[AT]GC[AT]^C
553 GAGCAC
BbvI 111 GCTGC GCAGC(8/12)
180 GCTGC
BsiHKAI 234 GAGCTC G[AT]GC[AT]^C
553 GAGCAC
BsmFI 191 GGGAC GGGAC(10/14)
299 GGGAC
BsrI 539 CCAGT ACTGG(1/-1)
577 CCAGT
BstUI 64 CGCG CG^CG
91 CGCG
DdeI 44 CTGAG C^T[ACGT]AG
106 CTGAG
DsaI 503 CCACGG C^C[GA][CT]GG
596 CCATGG
MboII 261 GAAGA GAAGA(8/7)
375 GAAGA
MvnI 64 CGCG CG^CG
91 CGCG
PflMI 249 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCACACCATGG
TseI 111 GCTGC G^C[AT]GC
180 GCTGC
Van91I 249 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCACACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaII BanII BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI Tsp45I