Restriction map for
pF1KB3652
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsiWI
BsmBI Bsp1407I BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstZ17I Bsu36I
CelII CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SexAI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 313 GTCTAC GT^[AC][GT]AC
AccII 2199 CGCG CG^CG
AccIII 896 TCCGGA T^CCGGA
Alw44I 712 GTGCAC G^TGCAC
AlwNI 731 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1770 GGGCCC GGGCC^C
ApaLI 712 GTGCAC G^TGCAC
Asp700I 2250 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2128 CCCGAG C^[CT]CG[GA]G
BalI 995 TGGCCA TGG^CCA
BanI 123 GGCACC G^G[CT][GA]CC
BbrPI 1438 CACGTG CAC^GTG
BbvCI 2174 CCTCAGC CCTCAGC(-5/-2)
BglII 468 AGATCT A^GATCT
BsaAI 1438 CACGTG [CT]AC^GT[GA]
BseAI 896 TCCGGA T^CCGGA
BsiEI 75 CGACCG CG[GA][CT]^CG
BsmI 824 GCATTC GAATGC(1/-1)
BsoBI 2128 CCCGAG C^[CT]CG[GA]G
BspDI 1184 ATCGAT AT^CGAT
BspEI 896 TCCGGA T^CCGGA
BspHI 810 TCATGA T^CATGA
BsrDI 748 GCAATG GCAATG(2/0)
BsrFI 655 GCCGGC [GA]^CCGG[CT]
BstBI 986 TTCGAA TT^CGAA
BstEII 27 GGTGACC G^GT[ACGT]ACC
BstPI 27 GGTGACC G^GT[ACGT]ACC
BstUI 2199 CGCG CG^CG
BstXI 1937 CCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 784 GCGC GCG^C
Cfr10I 655 GCCGGC [GA]^CCGG[CT]
ClaI 1184 ATCGAT AT^CGAT
DrdI 1015 GACAACTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 27 GGTGACC G^GT[ACGT]ACC
HaeII 783 GGCGCT [GA]GCGC^[CT]
HgaI 707 GCGTC GACGC(5/10)
HhaI 784 GCGC GCG^C
HinP1I 784 GCGC G^CGC
HindIII 1364 AAGCTT A^AGCTT
KspI 2198 CCGCGG CCGC^GG
MluNI 995 TGGCCA TGG^CCA
MroI 896 TCCGGA T^CCGGA
MscI 995 TGGCCA TGG^CCA
MslI 1140 CATGCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2199 CGCG CG^CG
NaeI 655 GCCGGC GCC^GGC
NgoMIV 655 GCCGGC G^CCGGC
NspV 986 TTCGAA TT^CGAA
PmaCI 1438 CACGTG CAC^GTG
PmlI 1438 CACGTG CAC^GTG
PstI 1453 CTGCAG CTGCA^G
PvuII 2177 CAGCTG CAG^CTG
RcaI 810 TCATGA T^CATGA
SacII 2198 CCGCGG CCGC^GG
SapI 2280 GAAGAGC GCTCTTC(1/4)
ScaI 101 AGTACT AGT^ACT
SfiI 1713 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 986 TTCGAA TT^CGAA
SmlI 105 CTTGAG C^T[CT][GA]AG
XmnI 2250 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 1126 GAATTT [GA]^AATT[CT]
1620 GAATTT
ApoI 1126 GAATTT [GA]^AATT[CT]
1620 GAATTT
AspHI 712 GTGCAC G[AT]GC[AT]^C
1761 GAGCAC
BglI 1714 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2149 GCCCCGGTGGC
BsgI 686 CTGCAC GTGCAG(16/14)
1001 GTGCAG
BsiHKAI 712 GTGCAC G[AT]GC[AT]^C
1761 GAGCAC
BssSI 14 CTCGTG CACGAG(-5/-1)
910 CTCGTG
BstAPI 676 GCAATTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
730 GCAGGGGCTGC
EaeI 995 TGGCCA [CT]^GGCC[GA]
1350 TGGCCG
EcoNI 132 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1689 CCTCGTCCAGG
FauI 1920 CCCGC CCCGC(4/6)
1323 GCGGG
NcoI 819 CCATGG C^CATGG
903 CCATGG
SfcI 1164 CTACAG C^T[GA][CT]AG
1453 CTGCAG
StuI 258 AGGCCT AGG^CCT
938 AGGCCT
XcmI 254 CCACAGGCCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1656 CCAGGTTTTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII