Restriction map for
pF1KB3758
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AclI AcsI AfaI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 517 GACGTC GACGT^C
AccIII 102 TCCGGA T^CCGGA
Alw44I 74 GTGCAC G^TGCAC
AocI 1326 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1187 AGCGCT AGC^GCT
ApaLI 74 GTGCAC G^TGCAC
AviII 114 TGCGCA TGC^GCA
BalI 1661 TGGCCA TGG^CCA
BamHI 151 GGATCC G^GATCC
BbeI 242 GGCGCC GGCGC^C
BsaI 1531 GAGACC GGTCTC(1/5)
BseAI 102 TCCGGA T^CCGGA
BsgI 916 CTGCAC GTGCAG(16/14)
BsmI 253 GCATTC GAATGC(1/-1)
BspEI 102 TCCGGA T^CCGGA
BsrBI 98 CCGCTC CCGCTC(-3/-3)
BsrDI 911 CATTGC GCAATG(2/0)
BssHII 438 GCGCGC G^CGCGC
BstYI 151 GGATCC [GA]^GATC[CT]
Bsu36I 1326 CCTAAGG CC^T[ACGT]AGG
DraIII 730 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1187 AGCGCT AGC^GCT
Eco81I 1326 CCTAAGG CC^T[ACGT]AGG
EcoRV 1408 GATATC GAT^ATC
FspI 114 TGCGCA TGC^GCA
KasI 242 GGCGCC G^GCGCC
MflI 151 GGATCC [GA]^GATC[CT]
MluNI 1661 TGGCCA TGG^CCA
MroI 102 TCCGGA T^CCGGA
MscI 1661 TGGCCA TGG^CCA
MslI 874 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 242 GGCGCC GG^CGCC
PleI 1203 GACTC GAGTC(4/5)
SexAI 731 ACCAGGT A^CC[AT]GGT
SfoI 242 GGCGCC GGC^GCC
SmlI 1021 CTTGAG C^T[CT][GA]AG
StuI 1001 AGGCCT AGG^CCT
Tsp45I 1591 GTGAC ^GT[GC]AC
XhoII 151 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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ApaI 1620 GGGCCC GGGCC^C
1736 GGGCCC
BglI 211 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1600 GCCCTGGAGGC
BlpI 698 GCTCAGC GC^T[ACGT]AGC
1090 GCTGAGC
Bpu1102I 698 GCTCAGC GC^T[ACGT]AGC
1090 GCTGAGC
BsmBI 1247 CGTCTC CGTCTC(1/5)
1222 GAGACG
BspMI 860 ACCTGC ACCTGC(4/8)
1362 GCAGGT
BstXI 556 CCACCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1610 CCAGCGGCTTGG
CelII 698 GCTCAGC GC^T[ACGT]AGC
1090 GCTGAGC
DsaI 307 CCGTGG C^C[GA][CT]GG
1731 CCACGG
HaeII 242 GGCGCC [GA]GCGC^[CT]
1187 AGCGCT
MaeIII 574 GTTAC ^GT[ACGT]AC
1591 GTGAC
NlaIII 1413 CATG CATG^
1638 CATG
PpuMI 53 GGGACCC [GA]G^G[AT]CC[CT]
605 GGGACCT
TaqI 1013 TCGA T^CGA
1356 TCGA
TfiI 772 GATTC G^A[AT]TC
981 GAATC
TthHB8I 1013 TCGA T^CGA
1356 TCGA
XcmI 327 CCAAGGGCCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1076 CCAGCCAACAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIV
PflMI PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TseI Tsp509I TspRI Van91I
XmaCI XmaI