Restriction map for
pF1KB3955
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# NO CUT ENZYMES ( 143 enzymes) #
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AccII AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FseI FspI HaeII HhaI
HinP1I HincII HindII HpaI KasI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 620 GACGTC GACGT^C
Acc65I 186 GGTACC G^GTACC
AccI 1162 GTCTAC GT^[AC][GT]AC
AcyI 620 GACGTC G[GA]^CG[CT]C
AflIII 1355 ACGTGT A^C[GA][CT]GT
ApaI 819 GGGCCC GGGCC^C
Asp700I 133 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 186 GGTACC G^GTACC
BanI 186 GGTACC G^G[CT][GA]CC
BanII 819 GGGCCC G[GA]GC[CT]^C
BbsI 1233 GAAGAC GAAGAC(2/6)
BclI 372 TGATCA T^GATCA
BcnI 486 CCGGG CC^[GC]GG
BmyI 819 GGGCCC G[AGT]GC[ACT]^C
BpmI 1148 CTGGAG CTGGAG(16/14)
BpuAI 1233 GAAGAC GAAGAC(2/6)
BsaAI 1354 TACGTG [CT]AC^GT[GA]
BsaHI 620 GACGTC G[GA]^CG[CT]C
BsmAI 872 GAGAC GTCTC(1/5)
BsmI 1058 GCATTC GAATGC(1/-1)
Bsp1286I 819 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 353 TGTACA T^GTACA
BspHI 120 TCATGA T^CATGA
BsrGI 353 TGTACA T^GTACA
BstEII 36 GGTCACC G^GT[ACGT]ACC
BstPI 36 GGTCACC G^GT[ACGT]ACC
EaeI 734 CGGCCA [CT]^GGCC[GA]
EcoO65I 36 GGTCACC G^GT[ACGT]ACC
EcoT22I 449 ATGCAT ATGCA^T
FbaI 372 TGATCA T^GATCA
HgaI 1221 GCGTC GACGC(5/10)
Hin1I 620 GACGTC G[GA]^CG[CT]C
HindIII 744 AAGCTT A^AGCTT
KpnI 186 GGTACC GGTAC^C
NciI 486 CCGGG CC^[GC]GG
NdeI 791 CATATG CA^TATG
NsiI 449 ATGCAT ATGCA^T
NspI 1307 ACATGC [GA]CATG^[CT]
PleI 796 GACTC GAGTC(4/5)
PpuMI 906 AGGACCC [GA]G^G[AT]CC[CT]
PstI 306 CTGCAG CTGCA^G
RcaI 120 TCATGA T^CATGA
SapI 958 GCTCTTC GCTCTTC(1/4)
SfcI 306 CTGCAG C^T[GA][CT]AG
SspBI 353 TGTACA T^GTACA
TfiI 124 GATTC G^A[AT]TC
XmnI 133 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 1180 GATCC GGATC(4/5)
928 GGATC
AvaII 546 GGACC G^G[AT]CC
907 GGACC
BglII 111 AGATCT A^GATCT
144 AGATCT
BseRI 755 CTCCTC GAGGAG(10/8)
1261 GAGGAG
BsmFI 435 GTCCC GGGAC(10/14)
853 GTCCC
BstYI 111 AGATCT [GA]^GATC[CT]
144 AGATCT
DraI 325 TTTAAA TTT^AAA
1018 TTTAAA
EarI 959 CTCTTC CTCTTC(1/4)
1036 GAAGAG
HapII 486 CCGG C^CGG
1312 CCGG
HinfI 124 GATTC G^A[ACGT]TC
796 GACTC
HpaII 486 CCGG C^CGG
1312 CCGG
HphI 38 TCACC GGTGA(8/7)
501 TCACC
Ksp632I 959 CTCTTC CTCTTC(1/4)
1036 GAAGAG
MflI 111 AGATCT [GA]^GATC[CT]
144 AGATCT
MslI 426 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1285 CACTTTGATG
MspI 486 CCGG C^CGG
1312 CCGG
TaqI 866 TCGA T^CGA
1005 TCGA
Tsp45I 37 GTCAC ^GT[GC]AC
1250 GTCAC
TthHB8I 866 TCGA T^CGA
1005 TCGA
XhoII 111 AGATCT [GA]^GATC[CT]
144 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
ItaI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp509I TspRI