Restriction map for
pF1KB3987
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BseAI BsgI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
HpaI KpnI KspI MaeIII MfeI
MluI MluNI MroI MscI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
SacI SacII SalI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp45I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 976 GACGTC GACGT^C
AlwNI 24 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 983 AGCGCT AGC^GCT
ApaI 56 GGGCCC GGGCC^C
AspI 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 230 TGCGCA TGC^GCA
BanI 164 GGCGCC G^G[CT][GA]CC
BbeI 164 GGCGCC GGCGC^C
BbsI 915 GAAGAC GAAGAC(2/6)
BbvCI 21 CCTCAGC CCTCAGC(-5/-2)
BglI 49 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 915 GAAGAC GAAGAC(2/6)
BsaBI 228 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 560 ACCGGA [AT]^CCGG[AT]
BseRI 756 CTCCTC GAGGAG(10/8)
BsmI 347 GCATTC GAATGC(1/-1)
BssSI 332 CACGAG CACGAG(-5/-1)
BstXI 800 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 158 CGGTCCG CG^G[AT]CCG
DsaI 240 CCACGG C^C[GA][CT]GG
Eco47III 983 AGCGCT AGC^GCT
EcoNI 780 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 598 GATATC GAT^ATC
FspI 230 TGCGCA TGC^GCA
HincII 841 GTCAAC GT[CT]^[GA]AC
HindII 841 GTCAAC GT[CT]^[GA]AC
HindIII 340 AAGCTT A^AGCTT
KasI 164 GGCGCC G^GCGCC
MamI 228 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 164 GGCGCC GG^CGCC
PflFI 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 776 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 834 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 158 CGGTCCG CG^G[AT]CCG
SapI 852 GCTCTTC GCTCTTC(1/4)
SbfI 1110 CCTGCAGG CCTGCA^GG
ScaI 539 AGTACT AGT^ACT
SfiI 48 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 164 GGCGCC GGC^GCC
Sse8387I 1110 CCTGCAGG CCTGCA^GG
TfiI 478 GATTC G^A[AT]TC
Tth111I 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 164 GGCGCC G[GA]^CG[CT]C
976 GACGTC
BanII 56 GGGCCC G[GA]GC[CT]^C
309 GGGCTC
BfaI 587 CTAG C^TAG
611 CTAG
BmyI 56 GGGCCC G[AGT]GC[ACT]^C
309 GGGCTC
BsaHI 164 GGCGCC G[GA]^CG[CT]C
976 GACGTC
BsaI 832 GAGACC GGTCTC(1/5)
753 GGTCTC
BsiEI 41 CGGCCG CG[GA][CT]^CG
243 CGGCCG
BsmBI 864 CGTCTC CGTCTC(1/5)
1014 GAGACG
BsmFI 451 GTCCC GGGAC(10/14)
696 GTCCC
Bsp1286I 56 GGGCCC G[AGT]GC[ACT]^C
309 GGGCTC
BsrBI 148 GAGCGG CCGCTC(-3/-3)
201 GAGCGG
BsrI 717 CCAGT ACTGG(1/-1)
996 CCAGT
BstYI 176 AGATCC [GA]^GATC[CT]
659 AGATCC
DdeI 22 CTCAG C^T[ACGT]AG
460 CTGAG
DraII 55 GGGGCCC [GA]G^G[ACGT]CC[CT]
776 AGGTCCT
EagI 41 CGGCCG C^GGCCG
243 CGGCCG
EclXI 41 CGGCCG C^GGCCG
243 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
243 CGGCCG
EcoO109I 55 GGGGCCC [GA]G^G[ACGT]CC[CT]
776 AGGTCCT
EcoT14I 352 CCTTGG C^C[AT][AT]GG
623 CCAAGG
HgaI 91 GACGC GACGC(5/10)
130 GACGC
Hin1I 164 GGCGCC G[GA]^CG[CT]C
976 GACGTC
MaeI 587 CTAG C^TAG
611 CTAG
MflI 176 AGATCC [GA]^GATC[CT]
659 AGATCC
MseI 653 TTAA T^TAA
668 TTAA
PleI 529 GAGTC GAGTC(4/5)
814 GAGTC
PstI 1111 CTGCAG CTGCA^G
1138 CTGCAG
SexAI 773 ACCAGGT A^CC[AT]GGT
989 ACCTGGT
SfcI 1111 CTGCAG C^T[GA][CT]AG
1138 CTGCAG
StyI 352 CCTTGG C^C[AT][AT]GG
623 CCAAGG
Tru9I 653 TTAA T^TAA
668 TTAA
Tsp509I 577 AATT ^AATT
630 AATT
TspRI 718 CAGTG CA[GC]TG[ACGT][ACGT]^
997 CAGTG
XhoII 176 AGATCC [GA]^GATC[CT]
659 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AvaII BbvI BcnI BpmI
BsaJI BsiYI BslI BsmAI BsrFI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TseI
TthHB8I XcmI XmnI