Restriction map for
pF1KB3990
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AvrII BbeI BbvCI BcgI
BciVI BfaI BglII BlnI BsiWI
BsmBI BspHI BsrDI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
MaeI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1785 GGTACC G^GTACC
AccIII 3083 TCCGGA T^CCGGA
AcsI 3072 GAATTC [GA]^AATT[CT]
AflII 258 CTTAAG C^TTAAG
Alw44I 721 GTGCAC G^TGCAC
ApaLI 721 GTGCAC G^TGCAC
ApoI 3072 GAATTC [GA]^AATT[CT]
Asp718I 1785 GGTACC G^GTACC
BamHI 890 GGATCC G^GATCC
BbrPI 871 CACGTG CAC^GTG
BbsI 2092 GAAGAC GAAGAC(2/6)
BclI 2837 TGATCA T^GATCA
BfrI 258 CTTAAG C^TTAAG
BpuAI 2092 GAAGAC GAAGAC(2/6)
BsaAI 871 CACGTG [CT]AC^GT[GA]
BsaBI 1888 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2464 GGTCTC GGTCTC(1/5)
BsaWI 3083 TCCGGA [AT]^CCGG[AT]
BseAI 3083 TCCGGA T^CCGGA
BsmI 1045 GCATTC GAATGC(1/-1)
Bsp1407I 1079 TGTACA T^GTACA
BspDI 934 ATCGAT AT^CGAT
BspEI 3083 TCCGGA T^CCGGA
BspLU11I 704 ACATGT A^CATGT
BsrGI 1079 TGTACA T^GTACA
BssHII 1599 GCGCGC G^CGCGC
BstBI 2314 TTCGAA TT^CGAA
ClaI 934 ATCGAT AT^CGAT
DraIII 871 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 437 CGGCCG C^GGCCG
EclXI 437 CGGCCG C^GGCCG
Eco52I 437 CGGCCG C^GGCCG
EcoRI 3072 GAATTC G^AATTC
FbaI 2837 TGATCA T^GATCA
KpnI 1785 GGTACC GGTAC^C
MamI 1888 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3083 TCCGGA T^CCGGA
NaeI 330 GCCGGC GCC^GGC
NgoMIV 330 GCCGGC G^CCGGC
NspV 2314 TTCGAA TT^CGAA
PaeR7I 2595 CTCGAG C^TCGAG
PmaCI 871 CACGTG CAC^GTG
PmlI 871 CACGTG CAC^GTG
PpuMI 1742 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2458 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1242 GAGCTC GAGCT^C
ScaI 1568 AGTACT AGT^ACT
SfuI 2314 TTCGAA TT^CGAA
SspBI 1079 TGTACA T^GTACA
SspI 1195 AATATT AAT^ATT
TfiI 1383 GAATC G^A[AT]TC
XhoI 2595 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 704 ACATGT A^C[GA][CT]GT
1057 ACGTGT
ApaI 794 GGGCCC GGGCC^C
1466 GGGCCC
Asp700I 2356 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2952 GAAGCTGTTC
BalI 1391 TGGCCA TGG^CCA
2868 TGGCCA
BlpI 1312 GCTGAGC GC^T[ACGT]AGC
2262 GCTGAGC
Bpu1102I 1312 GCTGAGC GC^T[ACGT]AGC
2262 GCTGAGC
BsgI 1176 CTGCAC GTGCAG(16/14)
2808 GTGCAG
BsrFI 330 GCCGGC [GA]^CCGG[CT]
2631 ACCGGC
BstAPI 1013 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1575 GCAGCGGCTGC
BstXI 958 CCAGCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1065 CCAGAAGGCTGG
BstYI 890 GGATCC [GA]^GATC[CT]
2750 GGATCT
CelII 1312 GCTGAGC GC^T[ACGT]AGC
2262 GCTGAGC
Cfr10I 330 GCCGGC [GA]^CCGG[CT]
2631 ACCGGC
KspI 1952 CCGCGG CCGC^GG
2071 CCGCGG
MflI 890 GGATCC [GA]^GATC[CT]
2750 GGATCT
MluNI 1391 TGGCCA TGG^CCA
2868 TGGCCA
MscI 1391 TGGCCA TGG^CCA
2868 TGGCCA
MslI 654 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1083 CATGAAAATG
NcoI 1676 CCATGG C^CATGG
1817 CCATGG
PvuII 1729 CAGCTG CAG^CTG
2149 CAGCTG
SacII 1952 CCGCGG CCGC^GG
2071 CCGCGG
XhoII 890 GGATCC [GA]^GATC[CT]
2750 GGATCT
XmnI 2356 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2952 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AspI AvaI
AvaII AviII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I