Restriction map for
pF1KB4000
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaWI BseAI BseRI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsaI RsrII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 228 CGCG CG^CG
AcyI 130 GACGCC G[GA]^CG[CT]C
AvaI 349 CCCGAG C^[CT]CG[GA]G
BanI 88 GGTGCC G^G[CT][GA]CC
BanII 352 GAGCTC G[GA]GC[CT]^C
BbrPI 373 CACGTG CAC^GTG
BbvCI 252 GCTGAGG CCTCAGC(-5/-2)
BsaHI 130 GACGCC G[GA]^CG[CT]C
BsaI 544 GGTCTC GGTCTC(1/5)
BsiEI 497 CGATCG CG[GA][CT]^CG
BsmAI 545 GTCTC GTCTC(1/5)
BsoBI 349 CCCGAG C^[CT]CG[GA]G
BspMI 103 ACCTGC ACCTGC(4/8)
BstAPI 201 GCAACACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 279 GGATG GGATG(2/0)
BstUI 228 CGCG CG^CG
EcoT14I 428 CCTTGG C^C[AT][AT]GG
FauI 229 GCGGG CCCGC(4/6)
FokI 279 GGATG GGATG(9/13)
HgaI 130 GACGC GACGC(5/10)
Hin1I 130 GACGCC G[GA]^CG[CT]C
HinfI 534 GACTC G^A[ACGT]TC
HphI 575 TCACC GGTGA(8/7)
KspI 227 CCGCGG CCGC^GG
MslI 276 CACGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 228 CGCG CG^CG
NspI 365 GCATGC [GA]CATG^[CT]
PleI 534 GACTC GAGTC(4/5)
PmaCI 373 CACGTG CAC^GTG
PmlI 373 CACGTG CAC^GTG
PstI 36 CTGCAG CTGCA^G
PvuI 497 CGATCG CGAT^CG
SacI 352 GAGCTC GAGCT^C
SacII 227 CCGCGG CCGC^GG
SfcI 36 CTGCAG C^T[GA][CT]AG
SmlI 355 CTCAAG C^T[CT][GA]AG
SphI 365 GCATGC GCATG^C
StyI 428 CCTTGG C^C[AT][AT]GG
Tsp509I 636 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AspHI 352 GAGCTC G[AT]GC[AT]^C
503 GAGCAC
AvaII 345 GGTCC G^G[AT]CC
395 GGACC
BsaAI 148 TACGTG [CT]AC^GT[GA]
373 CACGTG
BsiHKAI 352 GAGCTC G[AT]GC[AT]^C
503 GAGCAC
BsiYI 134 CCTTCCCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCCCAACACGG
BslI 134 CCTTCCCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCCCAACACGG
BssKI 139 CCAGG ^CC[ACGT]GG
333 CCAGG
BstNI 139 CCAGG CC^[AT]GG
333 CCAGG
DraII 344 GGGTCCC [GA]G^G[ACGT]CC[CT]
394 GGGACCC
DsaI 92 CCGTGG C^C[GA][CT]GG
227 CCGCGG
EcoO109I 344 GGGTCCC [GA]G^G[ACGT]CC[CT]
394 GGGACCC
EcoRII 139 CCAGG ^CC[AT]GG
333 CCAGG
HaeIII 257 GGCC GG^CC
432 GGCC
MaeII 149 ACGT A^CGT
374 ACGT
MvaI 139 CCAGG CC^[AT]GG
333 CCAGG
NlaIII 366 CATG CATG^
475 CATG
PpuMI 344 GGGTCCC [GA]G^G[AT]CC[CT]
394 GGGACCC
ScrFI 139 CCAGG CC^[ACGT]GG
333 CCAGG
Tsp45I 184 GTGAC ^GT[GC]AC
620 GTCAC
TspRI 156 CACTG CA[GC]TG[ACGT][ACGT]^
508 CAGTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AlwNI BbvI BmyI
BsaJI BsmFI Bsp1286I Cac8I Cfr13I
DdeI DpnII Fnu4HI ItaI MaeIII
MboI MboII MnlI MspA1I MwoI
NdeII NlaIV PvuII Sau3AI Sau96I
TseI