Restriction map for
pF1KB4046
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AocI ApaI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BlnI BsaAI
BsaI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BstBI
BstEII BstPI Bsu36I ClaI CpoI
DraI DraII DraIII DrdI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp509I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AfaI 660 GTAC GT^AC
AflII 1219 CTTAAG C^TTAAG
AhdI 1216 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1015 GTGCAC G^TGCAC
AlwI 1321 GATCC GGATC(4/5)
AlwNI 95 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 121 AGCGCT AGC^GCT
ApaLI 1015 GTGCAC G^TGCAC
Asp700I 157 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1216 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 152 CCCGGG C^[CT]CG[GA]G
BalI 847 TGGCCA TGG^CCA
BbeI 1293 GGCGCC GGCGC^C
BbsI 586 GTCTTC GAAGAC(2/6)
BfaI 1325 CTAG C^TAG
BfrI 1219 CTTAAG C^TTAAG
BglI 840 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 920 AGATCT A^GATCT
BpuAI 586 GTCTTC GAAGAC(2/6)
BsaBI 315 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1130 ACCGGA [AT]^CCGG[AT]
BsgI 1156 GTGCAG GTGCAG(16/14)
BsmI 60 GAATGC GAATGC(1/-1)
BsoBI 152 CCCGGG C^[CT]CG[GA]G
BsrBI 196 GAGCGG CCGCTC(-3/-3)
BsrDI 507 CATTGC GCAATG(2/0)
BsrFI 306 GCCGGC [GA]^CCGG[CT]
BssHII 556 GCGCGC G^CGCGC
BssSI 433 CACGAG CACGAG(-5/-1)
Bst1107I 1161 GTATAC GTA^TAC
BstAPI 1008 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 920 AGATCT [GA]^GATC[CT]
BstZ17I 1161 GTATAC GTA^TAC
Cfr10I 306 GCCGGC [GA]^CCGG[CT]
Eam1105I 1216 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 121 AGCGCT AGC^GCT
EcoT14I 1193 CCAAGG C^C[AT][AT]GG
KasI 1293 GGCGCC G^GCGCC
MaeI 1325 CTAG C^TAG
MaeII 1306 ACGT A^CGT
MamI 315 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 920 AGATCT [GA]^GATC[CT]
MluNI 847 TGGCCA TGG^CCA
MscI 847 TGGCCA TGG^CCA
MseI 1220 TTAA T^TAA
NaeI 306 GCCGGC GCC^GGC
NarI 1293 GGCGCC GG^CGCC
NgoMIV 306 GCCGGC G^CCGGC
NlaIII 1209 CATG CATG^
PflMI 94 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 660 GTAC GT^AC
SapI 399 GCTCTTC GCTCTTC(1/4)
ScaI 659 AGTACT AGT^ACT
SfoI 1293 GGCGCC GGC^GCC
SgrAI 305 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 152 CCCGGG CCC^GGG
StyI 1193 CCAAGG C^C[AT][AT]GG
Tru9I 1220 TTAA T^TAA
Van91I 94 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 850 CCAAAGGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 920 AGATCT [GA]^GATC[CT]
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
XmnI 157 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1084 GTCTAC GT^[AC][GT]AC
1161 GTATAC
BlpI 394 GCTGAGC GC^T[ACGT]AGC
829 GCTGAGC
Bpu1102I 394 GCTGAGC GC^T[ACGT]AGC
829 GCTGAGC
BseRI 73 CTCCTC GAGGAG(10/8)
436 GAGGAG
BsiEI 611 CGGCCG CG[GA][CT]^CG
880 CGGCCG
BsmAI 439 GAGAC GTCTC(1/5)
712 GTCTC
BsmBI 711 CGTCTC CGTCTC(1/5)
439 GAGACG
BsmFI 863 GGGAC GGGAC(10/14)
1172 GGGAC
BspMI 1043 ACCTGC ACCTGC(4/8)
1104 GCAGGT
BsrI 869 ACTGG ACTGG(1/-1)
1266 CCAGT
BstXI 1193 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1300 CCACTGACGTGG
CelII 394 GCTGAGC GC^T[ACGT]AGC
829 GCTGAGC
EagI 611 CGGCCG C^GGCCG
880 CGGCCG
EarI 76 CTCTTC CTCTTC(1/4)
400 CTCTTC
EclXI 611 CGGCCG C^GGCCG
880 CGGCCG
Eco52I 611 CGGCCG C^GGCCG
880 CGGCCG
HinfI 162 GATTC G^A[ACGT]TC
315 GATTC
Ksp632I 76 CTCTTC CTCTTC(1/4)
400 CTCTTC
KspI 263 CCGCGG CCGC^GG
608 CCGCGG
MslI 507 CATTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1301 CACTGACGTG
PvuII 1027 CAGCTG CAG^CTG
1099 CAGCTG
SacI 397 GAGCTC GAGCT^C
820 GAGCTC
SacII 263 CCGCGG CCGC^GG
608 CCGCGG
SfaNI 229 GATGC GCATC(5/9)
873 GATGC
TfiI 162 GATTC G^A[AT]TC
315 GATTC
TspRI 348 CACTG CA[GC]TG[ACGT][ACGT]^
1301 CACTG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
SmlI StuI TaqI TseI Tsp45I
TthHB8I