Restriction map for
pF1KB4095
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BbsI BcgI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaWI BseAI BsiWI BsmI BspEI
BspHI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV FseI HpaI KpnI
KspI MfeI MluI MroI MunI
MvnI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RcaI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 76 GTAGAC GT^[AC][GT]AC
AcsI 1479 GAATTT [GA]^AATT[CT]
AhdI 2578 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 124 CCTGAGG CC^T[ACGT]AGG
ApaI 2131 GGGCCC GGGCC^C
ApoI 1479 GAATTT [GA]^AATT[CT]
AspEI 2578 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 797 TGGCCA TGG^CCA
BciVI 2967 GGATAC GTATCC(6/5)
BclI 1130 TGATCA T^GATCA
BsaI 1654 GAGACC GGTCTC(1/5)
BsiEI 927 CGATCG CG[GA][CT]^CG
BsmBI 442 GAGACG CGTCTC(1/5)
Bsp1407I 2639 TGTACA T^GTACA
BspDI 316 ATCGAT AT^CGAT
BsrFI 2631 GCCGGC [GA]^CCGG[CT]
BsrGI 2639 TGTACA T^GTACA
BstEII 2925 GGTAACC G^GT[ACGT]ACC
BstPI 2925 GGTAACC G^GT[ACGT]ACC
Bsu36I 124 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2631 GCCGGC [GA]^CCGG[CT]
ClaI 316 ATCGAT AT^CGAT
Eam1105I 2578 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 124 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2925 GGTAACC G^GT[ACGT]ACC
EcoT22I 1510 ATGCAT ATGCA^T
FbaI 1130 TGATCA T^GATCA
HindIII 1845 AAGCTT A^AGCTT
MluNI 797 TGGCCA TGG^CCA
MscI 797 TGGCCA TGG^CCA
NaeI 2631 GCCGGC GCC^GGC
NgoMIV 2631 GCCGGC G^CCGGC
NheI 2809 GCTAGC G^CTAGC
NsiI 1510 ATGCAT ATGCA^T
PshAI 2988 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 927 CGATCG CGAT^CG
SbfI 1293 CCTGCAGG CCTGCA^GG
Sse8387I 1293 CCTGCAGG CCTGCA^GG
SspBI 2639 TGTACA T^GTACA
Tsp509I 1480 AATT ^AATT
XbaI 297 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 1364 ACATGT A^C[GA][CT]GT
1673 ACATGT
AviII 578 TGCGCA TGC^GCA
2117 TGCGCA
BamHI 513 GGATCC G^GATCC
1614 GGATCC
BbeI 2255 GGCGCC GGCGC^C
2730 GGCGCC
BbrPI 688 CACGTG CAC^GTG
1417 CACGTG
BbvCI 1308 CCTCAGC CCTCAGC(-5/-2)
2482 GCTGAGG
BspLU11I 1364 ACATGT A^CATGT
1673 ACATGT
BsrBI 85 GAGCGG CCGCTC(-3/-3)
1231 GAGCGG
BstAPI 489 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
744 GCAGATGGTGC
DraIII 1912 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2722 CACCAGGTG
EcoNI 2279 CCTATGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3120 CCTGGGCGAGG
FspI 578 TGCGCA TGC^GCA
2117 TGCGCA
HaeII 2255 GGCGCC [GA]GCGC^[CT]
2730 GGCGCC
HincII 244 GTTGAC GT[CT]^[GA]AC
2596 GTCAAC
HindII 244 GTTGAC GT[CT]^[GA]AC
2596 GTCAAC
KasI 2255 GGCGCC G^GCGCC
2730 GGCGCC
MseI 983 TTAA T^TAA
1787 TTAA
NarI 2255 GGCGCC GG^CGCC
2730 GGCGCC
NcoI 213 CCATGG C^CATGG
2795 CCATGG
PmaCI 688 CACGTG CAC^GTG
1417 CACGTG
PmlI 688 CACGTG CAC^GTG
1417 CACGTG
PvuII 493 CAGCTG CAG^CTG
1609 CAGCTG
SapI 82 GAAGAGC GCTCTTC(1/4)
118 GAAGAGC
SexAI 1913 ACCTGGT A^CC[AT]GGT
2723 ACCAGGT
SfoI 2255 GGCGCC GGC^GCC
2730 GGCGCC
SmaI 1532 CCCGGG CCC^GGG
2259 CCCGGG
TfiI 1439 GATTC G^A[AT]TC
1827 GATTC
Tru9I 983 TTAA T^TAA
1787 TTAA
XmaCI 1532 CCCGGG C^CCGGG
2259 CCCGGG
XmaI 1532 CCCGGG C^CCGGG
2259 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaBI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII