Restriction map for
pF1KB4280
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AhdI AlwI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
AspEI AvrII BalI BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 587 GGTACC G^GTACC
AccII 700 CGCG CG^CG
AcyI 301 GACGCC G[GA]^CG[CT]C
AfaI 588 GTAC GT^AC
AflIII 693 ACACGT A^C[GA][CT]GT
AgeI 584 ACCGGT A^CCGGT
Alw44I 720 GTGCAC G^TGCAC
ApaLI 720 GTGCAC G^TGCAC
Asp700I 114 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 587 GGTACC G^GTACC
AvaI 205 CCCGGG C^[CT]CG[GA]G
AvaII 582 GGACC G^G[AT]CC
AviII 371 TGCGCA TGC^GCA
BanI 587 GGTACC G^G[CT][GA]CC
BbrPI 694 CACGTG CAC^GTG
BbsI 562 GTCTTC GAAGAC(2/6)
BfaI 688 CTAG C^TAG
BglI 101 GCCTTATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 562 GTCTTC GAAGAC(2/6)
BsaAI 694 CACGTG [CT]AC^GT[GA]
BsaHI 301 GACGCC G[GA]^CG[CT]C
BsaWI 584 ACCGGT [AT]^CCGG[AT]
BsoBI 205 CCCGGG C^[CT]CG[GA]G
BsrFI 584 ACCGGT [GA]^CCGG[CT]
BstEII 483 GGTCACC G^GT[ACGT]ACC
BstPI 483 GGTCACC G^GT[ACGT]ACC
BstUI 700 CGCG CG^CG
BstXI 266 CCATGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 584 ACCGGT [GA]^CCGG[CT]
EcoNI 768 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 483 GGTCACC G^GT[ACGT]ACC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
FspI 371 TGCGCA TGC^GCA
Hin1I 301 GACGCC G[GA]^CG[CT]C
HinfI 309 GATTC G^A[ACGT]TC
KpnI 587 GGTACC GGTAC^C
MaeI 688 CTAG C^TAG
MvnI 700 CGCG CG^CG
NspI 195 GCATGC [GA]CATG^[CT]
PflMI 447 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 584 ACCGGT A^CCGGT
PmaCI 694 CACGTG CAC^GTG
PmlI 694 CACGTG CAC^GTG
RsaI 588 GTAC GT^AC
SfaNI 173 GCATC GCATC(5/9)
SmaI 205 CCCGGG CCC^GGG
SphI 195 GCATGC GCATG^C
StyI 161 CCAAGG C^C[AT][AT]GG
TfiI 309 GATTC G^A[AT]TC
Van91I 447 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 205 CCCGGG C^CCGGG
XmaI 205 CCCGGG C^CCGGG
XmnI 114 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 789 GGGCCC GGGCC^C
808 GGGCCC
AspHI 316 GAGCAC G[AT]GC[AT]^C
720 GTGCAC
AspI 478 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
556 GACATGGTC
BcnI 205 CCCGG CC^[GC]GG
206 CCGGG
BsiHKAI 316 GAGCAC G[AT]GC[AT]^C
720 GTGCAC
BsmFI 637 GGGAC GGGAC(10/14)
504 GTCCC
BspMI 143 ACCTGC ACCTGC(4/8)
590 ACCTGC
DdeI 76 CTGAG C^T[ACGT]AG
363 CTCAG
HgaI 301 GACGC GACGC(5/10)
502 GCGTC
MslI 267 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
486 CACCCTTGTG
NciI 205 CCCGG CC^[GC]GG
206 CCGGG
PflFI 478 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
556 GACATGGTC
PstI 127 CTGCAG CTGCA^G
190 CTGCAG
SfcI 127 CTGCAG C^T[GA][CT]AG
190 CTGCAG
TaqI 416 TCGA T^CGA
569 TCGA
Tth111I 478 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
556 GACATGGTC
TthHB8I 416 TCGA T^CGA
569 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI