Restriction map for
pF1KB4411
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI MwoI NarI NcoI
NdeI NheI NotI NruI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AfaI 522 GTAC GT^AC
ApaI 548 GGGCCC GGGCC^C
AvaI 413 CCCGGG C^[CT]CG[GA]G
BbsI 138 GAAGAC GAAGAC(2/6)
BbvCI 406 GCTGAGG CCTCAGC(-5/-2)
BglII 68 AGATCT A^GATCT
BpuAI 138 GAAGAC GAAGAC(2/6)
BsaI 35 GAGACC GGTCTC(1/5)
BsmFI 242 GTCCC GGGAC(10/14)
BsoBI 413 CCCGGG C^[CT]CG[GA]G
BsrFI 382 GCCGGC [GA]^CCGG[CT]
BsrI 148 CCAGT ACTGG(1/-1)
BstBI 478 TTCGAA TT^CGAA
BstYI 68 AGATCT [GA]^GATC[CT]
CfoI 424 GCGC GCG^C
Cfr10I 382 GCCGGC [GA]^CCGG[CT]
DpnII 69 GATC ^GATC
EaeI 380 TGGCCG [CT]^GGCC[GA]
EarI 178 CTCTTC CTCTTC(1/4)
EcoT22I 21 ATGCAT ATGCA^T
HaeII 423 GGCGCT [GA]GCGC^[CT]
HhaI 424 GCGC GCG^C
HinP1I 424 GCGC G^CGC
Ksp632I 178 CTCTTC CTCTTC(1/4)
MboI 69 GATC ^GATC
MflI 68 AGATCT [GA]^GATC[CT]
NaeI 382 GCCGGC GCC^GGC
NdeII 69 GATC ^GATC
NgoMIV 382 GCCGGC G^CCGGC
NlaIII 419 CATG CATG^
NsiI 21 ATGCAT ATGCA^T
NspV 478 TTCGAA TT^CGAA
PflMI 432 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 297 CTGCAG CTGCA^G
RsaI 522 GTAC GT^AC
Sau3AI 69 GATC ^GATC
SfcI 297 CTGCAG C^T[GA][CT]AG
SfuI 478 TTCGAA TT^CGAA
SmaI 413 CCCGGG CCC^GGG
TaqI 479 TCGA T^CGA
TfiI 283 GAATC G^A[AT]TC
Tsp509I 230 AATT ^AATT
TthHB8I 479 TCGA T^CGA
Van91I 432 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 17 CCAAATGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 68 AGATCT [GA]^GATC[CT]
XmaCI 413 CCCGGG C^CCGGG
XmaI 413 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 56 CCGC CCGC(-3/-1)
75 CCGC
AlwNI 149 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
433 CAGGGCCTG
BanII 86 GAGCCC G[GA]GC[CT]^C
548 GGGCCC
BbvI 13 GCAGC GCAGC(8/12)
299 GCAGC
BmyI 86 GAGCCC G[AGT]GC[ACT]^C
548 GGGCCC
BpmI 332 CTCCAG CTGGAG(16/14)
310 CTGGAG
Bsp1286I 86 GAGCCC G[AGT]GC[ACT]^C
548 GGGCCC
BstF5I 24 CATCC GGATG(2/0)
455 CATCC
DdeI 402 CTCAG C^T[ACGT]AG
407 CTGAG
DraII 434 AGGGCCT [GA]G^G[ACGT]CC[CT]
548 GGGCCCT
EcoO109I 434 AGGGCCT [GA]G^G[ACGT]CC[CT]
548 GGGCCCT
Fnu4HI 13 GCAGC GC^[ACGT]GC
299 GCAGC
FokI 24 CATCC GGATG(9/13)
455 CATCC
HphI 3 GGTGA GGTGA(8/7)
135 GGTGA
ItaI 13 GCAGC GC^[ACGT]GC
299 GCAGC
MaeIII 271 GTGAC ^GT[ACGT]AC
324 GTCAC
MnlI 409 GAGG CCTC(7/6)
538 GAGG
MspA1I 404 CAGCTG C[AC]G^C[GT]G
517 CAGCTG
PvuII 404 CAGCTG CAG^CTG
517 CAGCTG
SfaNI 23 GCATC GCATC(5/9)
338 GCATC
TseI 13 GCAGC G^C[AT]GC
299 GCAGC
Tsp45I 271 GTGAC ^GT[GC]AC
324 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BcnI BsaJI BsiYI BslI
BsmAI BssKI BstNI Cac8I Cfr13I
EcoRII HaeIII HapII HinfI HpaII
MboII MspI MvaI NciI NlaIV
PleI Sau96I ScrFI TspRI