Restriction map for
pF1KB4506
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
BalI BbeI BbrPI BbvCI BcgI
BciVI BfrI BlpI Bpu1102I BsaAI
BsaI BsiEI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KspI MfeI MluI MluNI MscI
MslI MspA1I MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
TaqI TfiI Tth111I TthHB8I XhoI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 522 GGTACC G^GTACC
AccII 961 CGCG CG^CG
AccIII 856 TCCGGA T^CCGGA
AcyI 3 GGCGTC G[GA]^CG[CT]C
ApaI 1855 GGGCCC GGGCC^C
Asp718I 522 GGTACC G^GTACC
AspHI 1933 GAGCAC G[AT]GC[AT]^C
AvrII 486 CCTAGG C^CTAGG
BamHI 733 GGATCC G^GATCC
BanI 522 GGTACC G^G[CT][GA]CC
BanII 1855 GGGCCC G[GA]GC[CT]^C
BclI 609 TGATCA T^GATCA
BglII 1029 AGATCT A^GATCT
BlnI 486 CCTAGG C^CTAGG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BseAI 856 TCCGGA T^CCGGA
BsiHKAI 1933 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1089 TGTACA T^GTACA
BspEI 856 TCCGGA T^CCGGA
BsrGI 1089 TGTACA T^GTACA
BstAPI 394 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 961 CGCG CG^CG
DrdI 1442 GACCCATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 609 TGATCA T^GATCA
HaeII 973 GGCGCT [GA]GCGC^[CT]
Hin1I 3 GGCGTC G[GA]^CG[CT]C
KpnI 522 GGTACC GGTAC^C
MroI 856 TCCGGA T^CCGGA
MvnI 961 CGCG CG^CG
PstI 35 CTGCAG CTGCA^G
SfaNI 1231 GATGC GCATC(5/9)
SmlI 77 CTCAAG C^T[CT][GA]AG
SspBI 1089 TGTACA T^GTACA
StuI 326 AGGCCT AGG^CCT
Tsp509I 1083 AATT ^AATT
XbaI 1116 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 395 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
548 CAGTCACTG
BglI 244 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1697 GCCCTGGTGGC
BmyI 1855 GGGCCC G[AGT]GC[ACT]^C
1933 GAGCAC
BsaBI 1030 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1188 GATCCACATC
BsaWI 856 TCCGGA [AT]^CCGG[AT]
872 ACCGGA
BsgI 401 CTGCAC GTGCAG(16/14)
554 CTGCAC
Bsp1286I 1855 GGGCCC G[AGT]GC[ACT]^C
1933 GAGCAC
DraIII 1373 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1403 CACTCCGTG
EaeI 1767 TGGCCG [CT]^GGCC[GA]
1800 TGGCCG
EcoNI 1200 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1489 CCTCCAAGAGG
HgaI 959 GACGC GACGC(5/10)
4 GCGTC
HincII 446 GTCAAC GT[CT]^[GA]AC
680 GTCAAC
HindII 446 GTCAAC GT[CT]^[GA]AC
680 GTCAAC
HphI 1678 GGTGA GGTGA(8/7)
314 TCACC
MaeII 1538 ACGT A^CGT
1902 ACGT
MamI 1030 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1188 GATCCACATC
NcoI 1013 CCATGG C^CATGG
1445 CCATGG
NdeI 104 CATATG CA^TATG
350 CATATG
NspI 1391 GCATGC [GA]CATG^[CT]
1706 GCATGC
PflMI 373 CCAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1402 CCACTCCGTGG
SmaI 1502 CCCGGG CCC^GGG
1671 CCCGGG
SphI 1391 GCATGC GCATG^C
1706 GCATGC
Van91I 373 CCAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1402 CCACTCCGTGG
XmaCI 1502 CCCGGG C^CCGGG
1671 CCCGGG
XmaI 1502 CCCGGG C^CCGGG
1671 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BbsI BbvI BcnI BfaI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI SpeI StyI
Tru9I TseI Tsp45I TspRI XcmI
XhoII