################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI AvrII BbrPI BcgI BclI BfaI BfrI BlnI BsaAI BsaBI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KspI MaeI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1991 GGTACC G^GTACC AccII 139 CGCG CG^CG AhdI 940 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1991 GGTACC G^GTACC AspEI 940 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 644 TGCGCA TGC^GCA BalI 2513 TGGCCA TGG^CCA BamHI 2505 GGATCC G^GATCC BciVI 1932 GTATCC GTATCC(6/5) BglII 1821 AGATCT A^GATCT BsaI 2275 GAGACC GGTCTC(1/5) BsaWI 971 ACCGGA [AT]^CCGG[AT] BsiEI 199 CGGCCG CG[GA][CT]^CG BspLU11I 2420 ACATGT A^CATGT BspMI 1529 ACCTGC ACCTGC(4/8) BsrDI 464 CATTGC GCAATG(2/0) BsrFI 201 GCCGGT [GA]^CCGG[CT] BssSI 737 CTCGTG CACGAG(-5/-1) BstUI 139 CGCG CG^CG Cfr10I 201 GCCGGT [GA]^CCGG[CT] DraIII 1432 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1641 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 199 CGGCCG C^GGCCG Eam1105I 940 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 199 CGGCCG C^GGCCG Eco52I 199 CGGCCG C^GGCCG EcoNI 331 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 644 TGCGCA TGC^GCA HgaI 922 GCGTC GACGC(5/10) KpnI 1991 GGTACC GGTAC^C MaeII 2054 ACGT A^CGT MluNI 2513 TGGCCA TGG^CCA MscI 2513 TGGCCA TGG^CCA MvnI 139 CGCG CG^CG NruI 138 TCGCGA TCG^CGA PaeR7I 662 CTCGAG C^TCGAG SapI 2346 GCTCTTC GCTCTTC(1/4) SfiI 682 GGCCCAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 339 AGGCCT AGG^CCT XcmI 2640 CCAGGACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 662 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 73 GGCGCC G[GA]^CG[CT]C 2484 GGCGCC AfaI 1770 GTAC GT^AC 1992 GTAC AflIII 2054 ACGTGT A^C[GA][CT]GT 2420 ACATGT Asp700I 1371 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1782 GAAGGAGTTC AvaI 16 CCCGAG C^[CT]CG[GA]G 662 CTCGAG BbeI 73 GGCGCC GGCGC^C 2484 GGCGCC BbsI 1638 GAAGAC GAAGAC(2/6) 2185 GAAGAC BbvCI 2341 CCTCAGC CCTCAGC(-5/-2) 2295 GCTGAGG BpuAI 1638 GAAGAC GAAGAC(2/6) 2185 GAAGAC BsaHI 73 GGCGCC G[GA]^CG[CT]C 2484 GGCGCC BsoBI 16 CCCGAG C^[CT]CG[GA]G 662 CTCGAG BstXI 1501 CCACGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1995 CCATGCTGGTGG EaeI 199 CGGCCG [CT]^GGCC[GA] 2513 TGGCCA EcoRI 266 GAATTC G^AATTC 861 GAATTC Hin1I 73 GGCGCC G[GA]^CG[CT]C 2484 GGCGCC HphI 406 GGTGA GGTGA(8/7) 1467 TCACC KasI 73 GGCGCC G^GCGCC 2484 GGCGCC NarI 73 GGCGCC GG^CGCC 2484 GGCGCC NspI 2420 ACATGT [GA]CATG^[CT] 2538 GCATGT PflMI 337 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 810 CCAGGCTTTGG PleI 293 GACTC GAGTC(4/5) 1057 GACTC RsaI 1770 GTAC GT^AC 1992 GTAC SbfI 1530 CCTGCAGG CCTGCA^GG 1617 CCTGCAGG SfoI 73 GGCGCC GGC^GCC 2484 GGCGCC Sse8387I 1530 CCTGCAGG CCTGCA^GG 1617 CCTGCAGG Van91I 337 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 810 CCAGGCTTTGG XmnI 1371 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1782 GAAGGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApaI ApoI AspHI AvaII BanI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII