Restriction map for
pF1KB4783
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AvrII BamHI BbeI BbrPI
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BsgI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
HaeII HindIII HpaI KasI KspI
MamI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 671 GACGTC GACGT^C
Acc65I 1309 GGTACC G^GTACC
AccII 4 CGCG CG^CG
AccIII 177 TCCGGA T^CCGGA
AcyI 671 GACGTC G[GA]^CG[CT]C
Alw44I 1270 GTGCAC G^TGCAC
ApaLI 1270 GTGCAC G^TGCAC
Asp718I 1309 GGTACC G^GTACC
AviII 1076 TGCGCA TGC^GCA
BbsI 423 GAAGAC GAAGAC(2/6)
BciVI 1103 GTATCC GTATCC(6/5)
BclI 1232 TGATCA T^GATCA
BglII 650 AGATCT A^GATCT
BpuAI 423 GAAGAC GAAGAC(2/6)
BsaAI 1949 CACGTA [CT]AC^GT[GA]
BsaHI 671 GACGTC G[GA]^CG[CT]C
BseAI 177 TCCGGA T^CCGGA
BsmBI 1047 GAGACG CGTCTC(1/5)
BsmI 749 GAATGC GAATGC(1/-1)
BspEI 177 TCCGGA T^CCGGA
BspMI 546 GCAGGT ACCTGC(4/8)
BsrDI 402 CATTGC GCAATG(2/0)
BsrFI 1817 GCCGGT [GA]^CCGG[CT]
BstUI 4 CGCG CG^CG
Cfr10I 1817 GCCGGT [GA]^CCGG[CT]
DraII 615 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoNI 1341 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 615 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1107 CCATGG C^C[AT][AT]GG
FauI 1442 GCGGG CCCGC(4/6)
FbaI 1232 TGATCA T^GATCA
FspI 1076 TGCGCA TGC^GCA
HgaI 1049 GACGC GACGC(5/10)
Hin1I 671 GACGTC G[GA]^CG[CT]C
HphI 1328 TCACC GGTGA(8/7)
KpnI 1309 GGTACC GGTAC^C
MfeI 449 CAATTG C^AATTG
MroI 177 TCCGGA T^CCGGA
MslI 818 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1694 CAGCTG C[AC]G^C[GT]G
MunI 449 CAATTG C^AATTG
MvnI 4 CGCG CG^CG
NcoI 1107 CCATGG C^CATGG
NdeI 200 CATATG CA^TATG
PpuMI 615 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1114 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1694 CAGCTG CAG^CTG
ScaI 586 AGTACT AGT^ACT
SfcI 1184 CTACAG C^T[GA][CT]AG
SmlI 1724 CTCAAG C^T[CT][GA]AG
SspI 1029 AATATT AAT^ATT
StuI 802 AGGCCT AGG^CCT
StyI 1107 CCATGG C^C[AT][AT]GG
TfiI 624 GATTC G^A[AT]TC
XbaI 574 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 814 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1837 CAGCCACTG
BalI 838 TGGCCA TGG^CCA
1090 TGGCCA
BanII 139 GAGCTC G[GA]GC[CT]^C
1788 GAGCTC
BglI 804 GCCTGATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1381 GCCAATCAGGC
BsaWI 177 TCCGGA [AT]^CCGG[AT]
1469 ACCGGA
BstYI 650 AGATCT [GA]^GATC[CT]
776 AGATCC
CfoI 3 GCGC GCG^C
1077 GCGC
EarI 582 GAAGAG CTCTTC(1/4)
1375 GAAGAG
HhaI 3 GCGC GCG^C
1077 GCGC
HinP1I 3 GCGC G^CGC
1077 GCGC
HincII 790 GTTGAC GT[CT]^[GA]AC
1121 GTCAAC
HindII 790 GTTGAC GT[CT]^[GA]AC
1121 GTCAAC
Ksp632I 582 GAAGAG CTCTTC(1/4)
1375 GAAGAG
MflI 650 AGATCT [GA]^GATC[CT]
776 AGATCC
MluNI 838 TGGCCA TGG^CCA
1090 TGGCCA
MscI 838 TGGCCA TGG^CCA
1090 TGGCCA
MseI 461 TTAA T^TAA
1145 TTAA
PflMI 936 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCAACTAATGG
SacI 139 GAGCTC GAGCT^C
1788 GAGCTC
SfaNI 1074 GATGC GCATC(5/9)
1407 GATGC
Tru9I 461 TTAA T^TAA
1145 TTAA
Van91I 936 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCAACTAATGG
XhoII 650 AGATCT [GA]^GATC[CT]
776 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI