Restriction map for
pF1KB4822
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NarI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmlI SpeI SphI Sse8387I SspI
StuI SwaI Tsp509I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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ApaI 1012 GGGCCC GGGCC^C
BalI 894 TGGCCA TGG^CCA
BbsI 279 GAAGAC GAAGAC(2/6)
BfaI 1225 CTAG C^TAG
BglI 809 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1324 AGATCT A^GATCT
BlpI 921 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 921 GCTGAGC GC^T[ACGT]AGC
BpuAI 279 GAAGAC GAAGAC(2/6)
BsaI 1055 GAGACC GGTCTC(1/5)
BsgI 754 CTGCAC GTGCAG(16/14)
BsmAI 1055 GAGAC GTCTC(1/5)
Bsp1407I 371 TGTACA T^GTACA
BsrDI 311 GCAATG GCAATG(2/0)
BsrGI 371 TGTACA T^GTACA
BssSI 784 CTCGTG CACGAG(-5/-1)
CelII 921 GCTGAGC GC^T[ACGT]AGC
DraI 229 TTTAAA TTT^AAA
HaeII 1020 GGCGCT [GA]GCGC^[CT]
KspI 600 CCGCGG CCGC^GG
MaeI 1225 CTAG C^TAG
MluNI 894 TGGCCA TGG^CCA
MscI 894 TGGCCA TGG^CCA
MseI 230 TTAA T^TAA
MslI 838 CATACGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 749 GCCGGC GCC^GGC
NcoI 831 CCATGG C^CATGG
NgoMIV 749 GCCGGC G^CCGGC
NheI 1224 GCTAGC G^CTAGC
PvuII 343 CAGCTG CAG^CTG
SacII 600 CCGCGG CCGC^GG
SexAI 863 ACCAGGT A^CC[AT]GGT
SmaI 112 CCCGGG CCC^GGG
SnaBI 840 TACGTA TAC^GTA
SspBI 371 TGTACA T^GTACA
TfiI 1139 GATTC G^A[AT]TC
Tru9I 230 TTAA T^TAA
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 199 GACGCC G[GA]^CG[CT]C
847 GACGCC
AlwNI 76 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
865 CAGGTCCTG
AocI 385 CCTGAGG CC^T[ACGT]AGG
1272 CCTGAGG
AvaI 112 CCCGGG C^[CT]CG[GA]G
811 CCCGAG
BanII 967 GGGCTC G[GA]GC[CT]^C
1012 GGGCCC
BsaAI 840 TACGTA [CT]AC^GT[GA]
1210 CACGTA
BsaHI 199 GACGCC G[GA]^CG[CT]C
847 GACGCC
BsoBI 112 CCCGGG C^[CT]CG[GA]G
811 CCCGAG
BspMI 1063 ACCTGC ACCTGC(4/8)
492 GCAGGT
BsrBI 1125 CCGCTC CCGCTC(-3/-3)
334 GAGCGG
BsrFI 749 GCCGGC [GA]^CCGG[CT]
1034 ACCGGC
BstF5I 153 CATCC GGATG(2/0)
953 GGATG
BstXI 851 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCAACAAACTGG
Bsu36I 385 CCTGAGG CC^T[ACGT]AGG
1272 CCTGAGG
Cfr10I 749 GCCGGC [GA]^CCGG[CT]
1034 ACCGGC
EagI 18 CGGCCG C^GGCCG
1122 CGGCCG
EarI 208 CTCTTC CTCTTC(1/4)
138 GAAGAG
EclXI 18 CGGCCG C^GGCCG
1122 CGGCCG
Eco52I 18 CGGCCG C^GGCCG
1122 CGGCCG
Eco81I 385 CCTGAGG CC^T[ACGT]AGG
1272 CCTGAGG
EcoNI 783 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
858 CCTGGACCAGG
EcoT14I 731 CCTTGG C^C[AT][AT]GG
831 CCATGG
FokI 153 CATCC GGATG(9/13)
953 GGATG
Hin1I 199 GACGCC G[GA]^CG[CT]C
847 GACGCC
Ksp632I 208 CTCTTC CTCTTC(1/4)
138 GAAGAG
MaeIII 646 GTGAC ^GT[ACGT]AC
1147 GTGAC
PpuMI 425 AGGACCC [GA]G^G[AT]CC[CT]
866 AGGTCCT
SapI 138 GAAGAGC GCTCTTC(1/4)
207 GCTCTTC
StyI 731 CCTTGG C^C[AT][AT]GG
831 CCATGG
Tsp45I 646 GTGAC ^GT[GC]AC
1147 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspI AvaII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiEI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI Tth111I TthHB8I
XhoII