Restriction map for
pF1KB5166
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI MvnI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 206 GGCGCC G[GA]^CG[CT]C
AfaI 144 GTAC GT^AC
AlwI 66 GATCC GGATC(4/5)
Asp700I 378 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 280 TGCGCA TGC^GCA
BanI 206 GGCGCC G^G[CT][GA]CC
BbeI 206 GGCGCC GGCGC^C
BsaHI 206 GGCGCC G[GA]^CG[CT]C
BsaI 274 GGTCTC GGTCTC(1/5)
BsaJI 5 CCTCGG C^C[ACGT][ACGT]GG
BseRI 406 CTCCTC GAGGAG(10/8)
BsmFI 113 GGGAC GGGAC(10/14)
BsrFI 185 GCCGGC [GA]^CCGG[CT]
BssSI 151 CACGAG CACGAG(-5/-1)
BstYI 65 AGATCC [GA]^GATC[CT]
Cfr10I 185 GCCGGC [GA]^CCGG[CT]
EaeI 8 CGGCCA [CT]^GGCC[GA]
EarI 21 GAAGAG CTCTTC(1/4)
FauI 324 GCGGG CCCGC(4/6)
FspI 280 TGCGCA TGC^GCA
HaeII 206 GGCGCC [GA]GCGC^[CT]
Hin1I 206 GGCGCC G[GA]^CG[CT]C
HinfI 300 GATTC G^A[ACGT]TC
KasI 206 GGCGCC G^GCGCC
Ksp632I 21 GAAGAG CTCTTC(1/4)
MboII 21 GAAGA GAAGA(8/7)
MflI 65 AGATCC [GA]^GATC[CT]
MslI 382 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 185 GCCGGC GCC^GGC
NarI 206 GGCGCC GG^CGCC
NgoMIV 185 GCCGGC G^CCGGC
NlaIII 86 CATG CATG^
NspI 85 ACATGC [GA]CATG^[CT]
PstI 61 CTGCAG CTGCA^G
RsaI 144 GTAC GT^AC
SapI 21 GAAGAGC GCTCTTC(1/4)
ScaI 143 AGTACT AGT^ACT
SfaNI 283 GCATC GCATC(5/9)
SfcI 61 CTGCAG C^T[GA][CT]AG
SfoI 206 GGCGCC GGC^GCC
TfiI 300 GATTC G^A[AT]TC
TspRI 47 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 99 CCAGCAGAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 65 AGATCC [GA]^GATC[CT]
XmnI 378 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 166 AGCT AG^CT
233 AGCT
AvaII 30 GGACC G^G[AT]CC
114 GGACC
BsiYI 11 CCATCGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
33 CCCTTCAGAGG
BslI 11 CCATCGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
33 CCCTTCAGAGG
BsmAI 245 GAGAC GTCTC(1/5)
275 GTCTC
BssKI 27 CCTGG ^CC[ACGT]GG
348 CCTGG
BstNI 27 CCTGG CC^[AT]GG
348 CCTGG
Cac8I 98 GCCAGC GC[ACGT]^[ACGT]GC
185 GCCGGC
DpnII 66 GATC ^GATC
459 GATC
EcoRII 27 CCTGG ^CC[AT]GG
348 CCTGG
MaeIII 290 GTGAC ^GT[ACGT]AC
395 GTGAC
MboI 66 GATC ^GATC
459 GATC
MvaI 27 CCTGG CC^[AT]GG
348 CCTGG
NdeII 66 GATC ^GATC
459 GATC
Sau3AI 66 GATC ^GATC
459 GATC
ScrFI 27 CCTGG CC^[ACGT]GG
348 CCTGG
TaqI 14 TCGA T^CGA
137 TCGA
Tsp45I 290 GTGAC ^GT[GC]AC
395 GTGAC
TthHB8I 14 TCGA T^CGA
137 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI BbvI BsrI BstF5I CfoI
Cfr13I DdeI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MnlI MspI MwoI NlaIV
Sau96I TseI