Restriction map for
pF1KB5181
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BfrI BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoRI
EcoRV FseI FspI HgaI Hin1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1186 GGTACC G^GTACC
Aor51HI 812 AGCGCT AGC^GCT
Asp700I 1197 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1186 GGTACC G^GTACC
BalI 1541 TGGCCA TGG^CCA
BbvCI 1817 CCTCAGC CCTCAGC(-5/-2)
BclI 920 TGATCA T^GATCA
BfaI 1318 CTAG C^TAG
BlpI 1692 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1692 GCTGAGC GC^T[ACGT]AGC
BsaWI 1189 ACCGGA [AT]^CCGG[AT]
BsgI 652 GTGCAG GTGCAG(16/14)
BsiEI 538 CGGCCG CG[GA][CT]^CG
BsmI 696 GAATGC GAATGC(1/-1)
BspHI 794 TCATGA T^CATGA
BspMI 1421 ACCTGC ACCTGC(4/8)
BsrBI 820 GAGCGG CCGCTC(-3/-3)
BsrFI 976 GCCGGC [GA]^CCGG[CT]
BstEII 1900 GGTGACC G^GT[ACGT]ACC
BstPI 1900 GGTGACC G^GT[ACGT]ACC
CelII 1692 GCTGAGC GC^T[ACGT]AGC
Cfr10I 976 GCCGGC [GA]^CCGG[CT]
DraIII 1480 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 538 CGGCCG C^GGCCG
EclXI 538 CGGCCG C^GGCCG
Eco47III 812 AGCGCT AGC^GCT
Eco52I 538 CGGCCG C^GGCCG
EcoO65I 1900 GGTGACC G^GT[ACGT]ACC
EcoT22I 267 ATGCAT ATGCA^T
FbaI 920 TGATCA T^GATCA
HincII 958 GTCAAC GT[CT]^[GA]AC
HindII 958 GTCAAC GT[CT]^[GA]AC
HindIII 499 AAGCTT A^AGCTT
KpnI 1186 GGTACC GGTAC^C
MaeI 1318 CTAG C^TAG
MaeII 1667 ACGT A^CGT
MluNI 1541 TGGCCA TGG^CCA
MscI 1541 TGGCCA TGG^CCA
MseI 1799 TTAA T^TAA
NaeI 976 GCCGGC GCC^GGC
NcoI 1895 CCATGG C^CATGG
NgoMIV 976 GCCGGC G^CCGGC
NsiI 267 ATGCAT ATGCA^T
NspI 265 ACATGC [GA]CATG^[CT]
RcaI 794 TCATGA T^CATGA
SbfI 891 CCTGCAGG CCTGCA^GG
SmaI 1640 CCCGGG CCC^GGG
Sse8387I 891 CCTGCAGG CCTGCA^GG
Tru9I 1799 TTAA T^TAA
XmaCI 1640 CCCGGG C^CCGGG
XmaI 1640 CCCGGG C^CCGGG
XmnI 1197 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BanI 1042 GGCACC G^G[CT][GA]CC
1186 GGTACC
BbsI 613 GAAGAC GAAGAC(2/6)
1332 GAAGAC
BglI 758 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1497 GCCCCCCAGGC
BglII 392 AGATCT A^GATCT
680 AGATCT
BpuAI 613 GAAGAC GAAGAC(2/6)
1332 GAAGAC
DsaI 535 CCACGG C^C[GA][CT]GG
1895 CCATGG
EaeI 538 CGGCCG [CT]^GGCC[GA]
1541 TGGCCA
PleI 24 GACTC GAGTC(4/5)
1564 GAGTC
TfiI 38 GAATC G^A[AT]TC
1363 GAATC
XcmI 140 CCACCCGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1945 CCACCTGAAACCTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII