Restriction map for
pF1KB5211
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccI AccIII AflII AgeI
AhdI Alw44I AlwNI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 421 AACGTT AA^CGTT
AocI 87 CCTAAGG CC^T[ACGT]AGG
Aor51HI 866 AGCGCT AGC^GCT
ApaI 386 GGGCCC GGGCC^C
AspHI 855 GAGCAC G[AT]GC[AT]^C
BalI 254 TGGCCA TGG^CCA
BamHI 278 GGATCC G^GATCC
BciVI 575 GTATCC GTATCC(6/5)
BlpI 106 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 106 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 855 GAGCAC G[AT]GC[AT]^C
BsmI 734 GAATGC GAATGC(1/-1)
BspHI 342 TCATGA T^CATGA
Bsu36I 87 CCTAAGG CC^T[ACGT]AGG
CelII 106 GCTCAGC GC^T[ACGT]AGC
DsaI 392 CCGCGG C^C[GA][CT]GG
EaeI 254 TGGCCA [CT]^GGCC[GA]
EarI 283 CTCTTC CTCTTC(1/4)
Eco47III 866 AGCGCT AGC^GCT
Eco81I 87 CCTAAGG CC^T[ACGT]AGG
EcoNI 307 CCTTGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 838 GAATTC G^AATTC
EcoRV 145 GATATC GAT^ATC
EcoT14I 307 CCTTGG C^C[AT][AT]GG
Ksp632I 283 CTCTTC CTCTTC(1/4)
KspI 392 CCGCGG CCGC^GG
MluNI 254 TGGCCA TGG^CCA
MscI 254 TGGCCA TGG^CCA
MslI 543 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 540 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 122 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 421 AACGTT AA^CGTT
PvuII 366 CAGCTG CAG^CTG
RcaI 342 TCATGA T^CATGA
SacII 392 CCGCGG CCGC^GG
StyI 307 CCTTGG C^C[AT][AT]GG
Van91I 540 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 265 CCATTCTCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 397 GACGTC GACGT^C
915 GACGTC
AccII 393 CGCG CG^CG
930 CGCG
AcsI 703 AAATTT [GA]^AATT[CT]
838 GAATTC
AflIII 481 ACACGT A^C[GA][CT]GT
901 ACACGT
ApoI 703 AAATTT [GA]^AATT[CT]
838 GAATTC
AvaI 381 CCCGGG C^[CT]CG[GA]G
821 CCCGGG
AvaII 3 GGTCC G^G[AT]CC
123 GGACC
BanII 386 GGGCCC G[GA]GC[CT]^C
818 GAGCCC
BbrPI 273 CACGTG CAC^GTG
902 CACGTG
BbsI 452 GTCTTC GAAGAC(2/6)
961 GTCTTC
BpuAI 452 GTCTTC GAAGAC(2/6)
961 GTCTTC
BseRI 886 GAGGAG GAGGAG(10/8)
1093 GAGGAG
BsgI 582 CTGCAC GTGCAG(16/14)
1123 GTGCAG
BsmFI 122 GGGAC GGGAC(10/14)
937 GGGAC
BsoBI 381 CCCGGG C^[CT]CG[GA]G
821 CCCGGG
BspMI 331 ACCTGC ACCTGC(4/8)
313 GCAGGT
BsrFI 180 GCCGGC [GA]^CCGG[CT]
1084 GCCGGC
BstUI 393 CGCG CG^CG
930 CGCG
BstYI 63 AGATCC [GA]^GATC[CT]
278 GGATCC
Cfr10I 180 GCCGGC [GA]^CCGG[CT]
1084 GCCGGC
HaeII 847 AGCGCC [GA]GCGC^[CT]
866 AGCGCT
HincII 114 GTTGAC GT[CT]^[GA]AC
437 GTCAAC
HindII 114 GTTGAC GT[CT]^[GA]AC
437 GTCAAC
MflI 63 AGATCC [GA]^GATC[CT]
278 GGATCC
MvnI 393 CGCG CG^CG
930 CGCG
NaeI 180 GCCGGC GCC^GGC
1084 GCCGGC
NgoMIV 180 GCCGGC G^CCGGC
1084 GCCGGC
PleI 515 GACTC GAGTC(4/5)
998 GACTC
PmaCI 273 CACGTG CAC^GTG
902 CACGTG
PmlI 273 CACGTG CAC^GTG
902 CACGTG
PstI 363 CTGCAG CTGCA^G
972 CTGCAG
SfcI 363 CTGCAG C^T[GA][CT]AG
972 CTGCAG
SmaI 381 CCCGGG CCC^GGG
821 CCCGGG
SmlI 357 CTTGAG C^T[CT][GA]AG
843 CTTGAG
TfiI 324 GAATC G^A[AT]TC
339 GATTC
XhoII 63 AGATCC [GA]^GATC[CT]
278 GGATCC
XmaCI 381 CCCGGG C^CCGGG
821 CCCGGG
XmaI 381 CCCGGG C^CCGGG
821 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
BbvI BcnI BmyI BpmI BsaAI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I