Restriction map for
pF1KB5221
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AciI 804 GCGG CCGC(-3/-1)
AcsI 352 AAATTC [GA]^AATT[CT]
Alw44I 808 GTGCAC G^TGCAC
ApaLI 808 GTGCAC G^TGCAC
ApoI 352 AAATTC [GA]^AATT[CT]
AspHI 808 GTGCAC G[AT]GC[AT]^C
BamHI 974 GGATCC G^GATCC
BclI 722 TGATCA T^GATCA
BsaAI 433 TACGTG [CT]AC^GT[GA]
BsiHKAI 808 GTGCAC G[AT]GC[AT]^C
BspMI 696 GCAGGT ACCTGC(4/8)
BssKI 746 CCTGG ^CC[ACGT]GG
BstNI 746 CCTGG CC^[AT]GG
DraI 349 TTTAAA TTT^AAA
DraII 563 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 906 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 563 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 746 CCTGG ^CC[AT]GG
EcoRV 421 GATATC GAT^ATC
EcoT14I 567 CCTTGG C^C[AT][AT]GG
FauI 804 GCGGG CCCGC(4/6)
FbaI 722 TGATCA T^GATCA
MspA1I 19 CAGCTG C[AC]G^C[GT]G
MvaI 746 CCTGG CC^[AT]GG
PpuMI 563 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 19 CAGCTG CAG^CTG
ScrFI 746 CCTGG CC^[ACGT]GG
SfcI 764 CTGTAG C^T[GA][CT]AG
StyI 567 CCTTGG C^C[AT][AT]GG
TaqI 161 TCGA T^CGA
Tsp45I 916 GTGAC ^GT[GC]AC
TthHB8I 161 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 327 GGTCC G^G[AT]CC
564 GGTCC
BbvI 203 GCAGC GCAGC(8/12)
987 GCTGC
BmyI 290 GGGCAC G[AGT]GC[ACT]^C
808 GTGCAC
BsaJI 567 CCTTGG C^C[ACGT][ACGT]GG
746 CCTGGG
BsgI 640 CTGCAC GTGCAG(16/14)
956 GTGCAG
BsmAI 232 GTCTC GTCTC(1/5)
625 GTCTC
BsmFI 655 GGGAC GGGAC(10/14)
885 GGGAC
Bsp1286I 290 GGGCAC G[AGT]GC[ACT]^C
808 GTGCAC
BstF5I 743 CATCC GGATG(2/0)
852 GGATG
BstYI 189 GGATCT [GA]^GATC[CT]
974 GGATCC
EarI 934 GAAGAG CTCTTC(1/4)
951 GAAGAG
Fnu4HI 203 GCAGC GC^[ACGT]GC
987 GCTGC
FokI 743 CATCC GGATG(9/13)
852 GGATG
HaeIII 99 GGCC GG^CC
939 GGCC
ItaI 203 GCAGC GC^[ACGT]GC
987 GCTGC
Ksp632I 934 GAAGAG CTCTTC(1/4)
951 GAAGAG
MflI 189 GGATCT [GA]^GATC[CT]
974 GGATCC
MwoI 383 GCCCGAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
769 GCAGATTTGGC
NlaIV 563 GGGTCC GG[ACGT]^[ACGT]CC
974 GGATCC
TseI 203 GCAGC G^C[AT]GC
987 GCTGC
XhoII 189 GGATCT [GA]^GATC[CT]
974 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AlwI BbsI BpuAI BsiYI
BslI BsrI Cfr13I DdeI DpnII
HinfI HphI MaeII MaeIII MboI
MboII MnlI MseI NdeII NlaIII
PleI Sau3AI Sau96I TfiI Tru9I
Tsp509I TspRI