Restriction map for
pF1KB5311
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BbeI BbvCI BciVI BcnI
BglI BglII BlnI BsaHI BsiWI
BsmBI BspDI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MluI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 918 TCCGGA T^CCGGA
AflII 1327 CTTAAG C^TTAAG
Alw44I 1096 GTGCAC G^TGCAC
ApaLI 1096 GTGCAC G^TGCAC
Asp700I 817 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1096 GTGCAC G[AT]GC[AT]^C
AvaI 717 CCCGAG C^[CT]CG[GA]G
BbrPI 334 CACGTG CAC^GTG
BcgI 1171 GCAGAACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 789 TGATCA T^GATCA
BfrI 1327 CTTAAG C^TTAAG
BlpI 906 GCTCAGC GC^T[ACGT]AGC
BpmI 67 CTGGAG CTGGAG(16/14)
Bpu1102I 906 GCTCAGC GC^T[ACGT]AGC
BsaAI 334 CACGTG [CT]AC^GT[GA]
BsaBI 1122 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 152 GGTCTC GGTCTC(1/5)
BsaWI 918 TCCGGA [AT]^CCGG[AT]
BseAI 918 TCCGGA T^CCGGA
BsiEI 955 CGGCCG CG[GA][CT]^CG
BsiHKAI 1096 GTGCAC G[AT]GC[AT]^C
BsoBI 717 CCCGAG C^[CT]CG[GA]G
Bsp1407I 140 TGTACA T^GTACA
BspEI 918 TCCGGA T^CCGGA
BspHI 485 TCATGA T^CATGA
BsrDI 1197 CATTGC GCAATG(2/0)
BsrGI 140 TGTACA T^GTACA
BstXI 869 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 906 GCTCAGC GC^T[ACGT]AGC
DsaI 242 CCATGG C^C[GA][CT]GG
EagI 955 CGGCCG C^GGCCG
EclXI 955 CGGCCG C^GGCCG
Eco52I 955 CGGCCG C^GGCCG
EcoNI 157 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 293 GAATTC G^AATTC
FauI 544 GCGGG CCCGC(4/6)
FbaI 789 TGATCA T^GATCA
HaeII 48 GGCGCT [GA]GCGC^[CT]
HapII 919 CCGG C^CGG
HpaII 919 CCGG C^CGG
MaeII 335 ACGT A^CGT
MamI 1122 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 600 CAATTG C^AATTG
MroI 918 TCCGGA T^CCGGA
MslI 366 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 919 CCGG C^CGG
MunI 600 CAATTG C^AATTG
NcoI 242 CCATGG C^CATGG
PmaCI 334 CACGTG CAC^GTG
PmlI 334 CACGTG CAC^GTG
RcaI 485 TCATGA T^CATGA
SapI 235 GAAGAGC GCTCTTC(1/4)
SfaNI 111 GCATC GCATC(5/9)
SphI 38 GCATGC GCATG^C
SspBI 140 TGTACA T^GTACA
XcmI 1034 CCAATGAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 817 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 293 GAATTC [GA]^AATT[CT]
1084 AAATTT
AflIII 137 ACATGT A^C[GA][CT]GT
1070 ACATGT
ApoI 293 GAATTC [GA]^AATT[CT]
1084 AAATTT
BalI 287 TGGCCA TGG^CCA
767 TGGCCA
BanI 1078 GGCACC G^G[CT][GA]CC
1377 GGTGCC
BanII 238 GAGCCC G[GA]GC[CT]^C
1141 GAGCCC
BbsI 846 GAAGAC GAAGAC(2/6)
464 GTCTTC
BfaI 352 CTAG C^TAG
707 CTAG
BpuAI 846 GAAGAC GAAGAC(2/6)
464 GTCTTC
BseRI 627 CTCCTC GAGGAG(10/8)
1009 GAGGAG
BsgI 470 CTGCAC GTGCAG(16/14)
1359 GTGCAG
BsmAI 153 GTCTC GTCTC(1/5)
1384 GTCTC
BsmI 897 GAATGC GAATGC(1/-1)
835 GCATTC
BspLU11I 137 ACATGT A^CATGT
1070 ACATGT
BstAPI 72 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1236 GCAGCAGCTGC
BstYI 347 AGATCC [GA]^GATC[CT]
1103 AGATCC
CfoI 49 GCGC GCG^C
493 GCGC
EarI 235 GAAGAG CTCTTC(1/4)
966 GAAGAG
HhaI 49 GCGC GCG^C
493 GCGC
HinP1I 49 GCGC G^CGC
493 GCGC
Ksp632I 235 GAAGAG CTCTTC(1/4)
966 GAAGAG
MaeI 352 CTAG C^TAG
707 CTAG
MflI 347 AGATCC [GA]^GATC[CT]
1103 AGATCC
MluNI 287 TGGCCA TGG^CCA
767 TGGCCA
MscI 287 TGGCCA TGG^CCA
767 TGGCCA
MspA1I 394 CAGCTG C[AC]G^C[GT]G
1240 CAGCTG
PvuII 394 CAGCTG CAG^CTG
1240 CAGCTG
SmlI 7 CTCAAG C^T[CT][GA]AG
1327 CTTAAG
TaqI 781 TCGA T^CGA
1349 TCGA
TfiI 248 GAATC G^A[AT]TC
915 GAATC
TthHB8I 781 TCGA T^CGA
1349 TCGA
XhoII 347 AGATCC [GA]^GATC[CT]
1103 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI ScrFI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI