Restriction map for
pF1KB5318
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII ClaI CpoI DraI DraII
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FauI FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AhdI 763 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1172 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 763 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 383 GTGCTC G[AT]GC[AT]^C
AvaI 242 CTCGAG C^[CT]CG[GA]G
AviII 807 TGCGCA TGC^GCA
BalI 669 TGGCCA TGG^CCA
BanI 701 GGCACC G^G[CT][GA]CC
BbvI 708 GCTGC GCAGC(8/12)
BclI 741 TGATCA T^GATCA
BmyI 383 GTGCTC G[AGT]GC[ACT]^C
BsaJI 1156 CCTTGG C^C[ACGT][ACGT]GG
BseRI 34 GAGGAG GAGGAG(10/8)
BsgI 709 CTGCAC GTGCAG(16/14)
BsiHKAI 383 GTGCTC G[AT]GC[AT]^C
BsoBI 242 CTCGAG C^[CT]CG[GA]G
Bsp1286I 383 GTGCTC G[AGT]GC[ACT]^C
BspMI 704 ACCTGC ACCTGC(4/8)
BsrDI 414 CATTGC GCAATG(2/0)
BsrFI 868 ACCGGC [GA]^CCGG[CT]
BssSI 712 CACGAG CACGAG(-5/-1)
BstBI 934 TTCGAA TT^CGAA
BstEII 520 GGTCACC G^GT[ACGT]ACC
BstPI 520 GGTCACC G^GT[ACGT]ACC
CfoI 808 GCGC GCG^C
Cfr10I 868 ACCGGC [GA]^CCGG[CT]
DraIII 796 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 204 GACTCATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 669 TGGCCA [CT]^GGCC[GA]
Eam1105I 763 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 520 GGTCACC G^GT[ACGT]ACC
EcoRI 552 GAATTC G^AATTC
EcoT14I 1156 CCTTGG C^C[AT][AT]GG
FbaI 741 TGATCA T^GATCA
FspI 807 TGCGCA TGC^GCA
HapII 869 CCGG C^CGG
HhaI 808 GCGC GCG^C
HinP1I 808 GCGC G^CGC
HincII 823 GTCAAC GT[CT]^[GA]AC
HindII 823 GTCAAC GT[CT]^[GA]AC
HpaII 869 CCGG C^CGG
MaeII 211 ACGT A^CGT
MluNI 669 TGGCCA TGG^CCA
MscI 669 TGGCCA TGG^CCA
MslI 943 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 677 CAGCTG C[AC]G^C[GT]G
MspI 869 CCGG C^CGG
NspI 392 ACATGC [GA]CATG^[CT]
NspV 934 TTCGAA TT^CGAA
PaeR7I 242 CTCGAG C^TCGAG
PvuII 677 CAGCTG CAG^CTG
SapI 112 GCTCTTC GCTCTTC(1/4)
SfcI 846 CTACAG C^T[GA][CT]AG
SfuI 934 TTCGAA TT^CGAA
SmlI 242 CTCGAG C^T[CT][GA]AG
SspI 1216 AATATT AAT^ATT
StyI 1156 CCTTGG C^C[AT][AT]GG
TseI 708 GCTGC G^C[AT]GC
XhoI 242 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 247 GTAC GT^AC
1007 GTAC
Asp700I 853 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
985 GAAACTATTC
BfaI 193 CTAG C^TAG
862 CTAG
BpmI 1085 CTCCAG CTGGAG(16/14)
1258 CTGGAG
BsaI 541 GGTCTC GGTCTC(1/5)
971 GGTCTC
BsmAI 542 GTCTC GTCTC(1/5)
972 GTCTC
BstAPI 702 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1063 GCAGAAGATGC
EarI 113 CTCTTC CTCTTC(1/4)
1231 GAAGAG
HphI 304 GGTGA GGTGA(8/7)
522 TCACC
Ksp632I 113 CTCTTC CTCTTC(1/4)
1231 GAAGAG
MaeI 193 CTAG C^TAG
862 CTAG
NlaIV 701 GGCACC GG[ACGT]^[ACGT]CC
1142 GGAACC
RsaI 247 GTAC GT^AC
1007 GTAC
SexAI 95 ACCTGGT A^CC[AT]GGT
766 ACCAGGT
TaqI 243 TCGA T^CGA
935 TCGA
TthHB8I 243 TCGA T^CGA
935 TCGA
XmnI 853 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
985 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII PleI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
Tsp45I Tsp509I TspRI XhoII