Restriction map for
pF1KB5319
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 706 GTCTAC GT^[AC][GT]AC
AlwNI 1216 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 801 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 136 GGTGCC G^G[CT][GA]CC
BanII 715 GAGCTC G[GA]GC[CT]^C
BbvCI 442 CCTCAGC CCTCAGC(-5/-2)
BpmI 1213 CTCCAG CTGGAG(16/14)
BsaAI 1118 TACGTA [CT]AC^GT[GA]
BseRI 750 CTCCTC GAGGAG(10/8)
BsgI 1201 CTGCAC GTGCAG(16/14)
BsmFI 243 GGGAC GGGAC(10/14)
BsrBI 204 CCGCTC CCGCTC(-3/-3)
BstAPI 1175 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 685 CCAAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1001 AGATCC [GA]^GATC[CT]
CfoI 276 GCGC GCG^C
EarI 42 GAAGAG CTCTTC(1/4)
EcoRI 840 GAATTC G^AATTC
FauI 203 CCCGC CCCGC(4/6)
HaeII 275 GGCGCT [GA]GCGC^[CT]
HhaI 276 GCGC GCG^C
HinP1I 276 GCGC G^CGC
HincII 832 GTTAAC GT[CT]^[GA]AC
HindII 832 GTTAAC GT[CT]^[GA]AC
HinfI 520 GATTC G^A[ACGT]TC
HpaI 832 GTTAAC GTT^AAC
Ksp632I 42 GAAGAG CTCTTC(1/4)
MaeII 1119 ACGT A^CGT
MflI 1001 AGATCC [GA]^GATC[CT]
MspA1I 35 CAGCTG C[AC]G^C[GT]G
NspI 494 ACATGC [GA]CATG^[CT]
PflMI 1215 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 35 CAGCTG CAG^CTG
SacI 715 GAGCTC GAGCT^C
SmlI 162 CTTGAG C^T[CT][GA]AG
SnaBI 1118 TACGTA TAC^GTA
StuI 158 AGGCCT AGG^CCT
TfiI 520 GATTC G^A[AT]TC
Van91I 1215 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1001 AGATCC [GA]^GATC[CT]
XmnI 801 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 400 GTAC GT^AC
1112 GTAC
AlwI 784 GATCC GGATC(4/5)
1002 GATCC
AspHI 696 GTGCTC G[AT]GC[AT]^C
715 GAGCTC
BfaI 1007 CTAG C^TAG
1172 CTAG
BmyI 696 GTGCTC G[AGT]GC[ACT]^C
715 GAGCTC
BsaWI 236 ACCGGA [AT]^CCGG[AT]
836 ACCGGA
BsiHKAI 696 GTGCTC G[AT]GC[AT]^C
715 GAGCTC
BsmI 319 GAATGC GAATGC(1/-1)
919 GAATGC
Bsp1286I 696 GTGCTC G[AGT]GC[ACT]^C
715 GAGCTC
Cac8I 218 GCAGGC GC[ACGT]^[ACGT]GC
278 GCTGGC
Cfr13I 281 GGCCC G^G[ACGT]CC
1207 GGCCC
DsaI 140 CCATGG C^C[GA][CT]GG
1074 CCATGG
HapII 237 CCGG C^CGG
837 CCGG
HpaII 237 CCGG C^CGG
837 CCGG
MaeI 1007 CTAG C^TAG
1172 CTAG
MseI 833 TTAA T^TAA
1031 TTAA
MspI 237 CCGG C^CGG
837 CCGG
NcoI 140 CCATGG C^CATGG
1074 CCATGG
RsaI 400 GTAC GT^AC
1112 GTAC
Sau96I 281 GGCCC G^G[ACGT]CC
1207 GGCCC
Tru9I 833 TTAA T^TAA
1031 TTAA
XcmI 140 CCATGGAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
755 CCACAAGTGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HphI ItaI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
StyI TseI Tsp45I Tsp509I TspRI